A Cell Cycle-Regulated GATA Factor Promotes Centromeric Localization of CENP-A in Fission Yeast

Chen, Ee Sin; Saitoh, Shigeaki; Yanagida, Mitsuhiro; Takahashi, Kohske

doi:10.1016/s1097-2765(03)00011-x

Cited by 126 publications

(178 citation statements)

References 57 publications

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“…A genome wide RNAi screen for defects in Drosophila CenH3 localization at centromeres identified CAL1 and CENP-C as essential proteins for assembly of newly synthesized CenH3 (16). In S. pombe Mis 6 and Ams2 proteins are involved in CenH3 localization (17,18), Mis16 and Mis18 are required for CenH3 loading and Sim3 might act to escort CENP-A to centromere (19,20). The human proteins hMis18 and M18BP1, recruited to centromere at telophase-G1, and RbAp46/RbAp48 may act to prime centromere for CENP-A localization (21).…”

mentioning

confidence: 99%

HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres

Shuaib

Ouararhni

Dimitrov

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

177

186

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The human histone H3 variant, CENP-A, replaces the conventional histone H3 in centromeric chromatin and, together with centromerespecific DNA-binding factors, directs the assembly of the kinetochore. We purified the prenucelosomal e-CENP-A complex. We found that HJURP, a member of the complex, was required for cell cycle specific targeting of CENP-A to centromeres. HJURP facilitated efficient deposition of CENP-A/H4 tetramers to naked DNA in vitro. Bacterially expressed HJURP binds at a stoichiometric ratio to the CENP-A/H4 tetramer but not to the H3/H4 tetramer. The binding occurred through a conserved HJURP short N-terminal domain, termed CBD. The novel characteristic identified in vertebrates that we named TLTY box of CBD, was essential for formation of the HJURP-CENP-A/H4 complex. Our data identified HJURP as a vertebrate CENP-A chaperone and dissected its mode of interactions with CENP-A.histone chaperone | histone variant

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mentioning

confidence: 99%

HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres

Shuaib

Ouararhni

Dimitrov

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

177

186

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“…However, recent data suggest that transcription across centromeric repeats may contribute to centromere formation (18)(19)(20)(21)(22) and indicate that genes within centromeres can be transcribed (23,24). Transcription and͞or remodeling of the nucleosomes at the centromeres may also be important for the deposition of CENP-A (21, 22).…”

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confidence: 99%

Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function

Bouzinba-Ségard

Guais

Francastel

2006

Proc. Natl. Acad. Sci. U.S.A.

211

143

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RNAs have been implicated in the assembly and stabilization of large-scale chromatin structures including centromeric architecture; unidentified RNAs are integral components of human pericentric heterochromatin and are required for localization of the heterochromatin protein HP1 to centromeric regions. Because satellite repeats in centromeric regions are known to be transcribed, we assessed a role for noncoding centromeric RNAs in the structure and function of the centromere. We identified minor satellite transcripts of 120 nt in murine cells that localize to centromeres and accumulate upon stress or differentiation. Forced accumulation of 120-nt transcripts leads to defects in chromosome segregation and sister-chromatid cohesion, changes in hallmark centromeric epigenetic markers, and mislocalization of centromere-associated proteins essential for centromere function. These findings suggest that small centromeric RNAs may represent one of many pathways that regulate heterochromatin assembly in mammals, possibly through tethering of kinetochore-and heterochromatin-associated proteins.centromere ͉ centromeric transcripts ͉ kinetochore T he centromere is a highly specialized structure needed for faithful chromosome segregation during cell division and correct inheritance of genetic information (1). Centromeric regions provide an anchor point for the spindle apparatus, which pulls the separating chromosomes to opposite poles of the dividing cell. In addition, centromeres ensure that sister chromatids are kept together until the appropriate point in the division process.In the absence of conserved DNA sequences among species, two features are considered as a hallmark for centromeric regions, i.e., deposition of the histone H3 variant CENP-A (2, 3) and the constitutively heterochromatic nature of the region associated with the presence of long tandem repeats of short DNA sequences (4, 5). The mouse genome contains two classes of centromeric repetitive sequences, termed major and minor satellites, organized in largely uninterrupted blocks of tandem repeats. Cytological detection of these clusters shows distinct localization, major satellites being associated with pericentromeric regions, whereas minor satellites were detected at the primary constriction of condensed mitotic chromosomes (6, 7). The nature of protein complexes recruited to these satellite repeats also appears to be distinct. The constituents of the kinetochore are recruited, most directly by CENP-A, to minor satellites in a hierarchical and dynamic manner over the progression of the cell cycle (8), whereas heterochromatinassociated proteins, including the heterochromatin protein 1 (HP1), have been shown to mainly concentrate at pericentromeric regions (9). The constitutive heterochromatic state generally includes several typical epigenetic marks such as methylation of DNA (10) and methylation of the lysine 9 of histone H3 (11). These marks are, in turn, responsible for the binding of methyl-DNA-binding proteins and HP1 respectively (12-15).Other mechani...

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“…The fission yeast zinc finger protein Ams2 binds to the same region of DNA as the centromere-specific histone CENH3 and is required for its binding to DNA. (18) Building factors linked to histone methylation…”

Section: Noncoding Functional Rnamentioning

confidence: 99%

Heterochromatin?many flavours, common themes

Craig

2004

Bioessays

115

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SummaryHeterochromatin remains condensed throughout the cell cycle, is generally transcriptionally inert and is built and maintained by groups of factors with each group member sharing a similar function. In mammals, these groups include sequence-specific transcriptional repressors, functional RNA and proteins involved in DNA and histone methylation. Heterochromatin is cemented together via interactions within and between each protein group and is maintained by the cell's replication machinery. It can be constitutive (permanent) or facultative (developmentally regulated) and be any size, from a gene promotor to a whole genome. By studying the formation of facultative heterochromatin, we have gained information about how heterochromatin is assembled. We have discovered that there are many different architectural plans for the building of heterochromatin, leading to a seemingly never-ending variety of heterochromatic loci, with each built according to a general rule.

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A Cell Cycle-Regulated GATA Factor Promotes Centromeric Localization of CENP-A in Fission Yeast

Cited by 126 publications

References 57 publications

HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres

HJURP binds CENP-A via a highly conserved N-terminal domain and mediates its deposition at centromeres

Accumulation of small murine minor satellite transcripts leads to impaired centromeric architecture and function

Heterochromatin?many flavours, common themes

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