A Cell‐Targeted Non‐Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium‐Containing Cationic Radical Initiator

Uchiyama, Seiichi; Tsuji, Toshikazu; Kawamoto, Kyoko; Okano, Kentaro; Fukatsu, Eiko; Noro, Takahiro; Ikado, Kumiko; Yamada, Shoichi; Shibata, Yuka; Hayashi, Teruyuki; Inada, Noriko; Kato, Masaru; Koizumi, Hideki; Tokuyama, Hidetoshi

doi:10.1002/anie.201801495

Cited by 56 publications

(51 citation statements)

References 43 publications

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“…This would be because the rate constant for ADIP radical production is two orders of magnitude larger than AIBN, as indicated in a previous study. [ 21 ] We further investigated the characteristics of ADIP to determine the condition of the preparation of the gels using ADIP. The rate constant ( k d ) values for the thermal decomposition of ADIP in methanol were estimated by measuring the absorbance value of the methanol solution containing ADIP at 370 nm (Figure S1 in the Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Strong Cationic Radical Initiator‐Based Design of a Thermoresponsive Hydrogel Showing Drastic Volume Transition

Masuda

Tsuji

Koizumi

et al. 2020

Macro Chemistry & Physics

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In this study, a millimeter‐sized thermoresponsive poly(N‐isopropylacrylamide) (PNIPAAm) gel is prepared by free radical polymerization using 2,2′‐azobis‐[2‐(1,3‐dimethyl‐4,5‐dihydro‐1H‐imidazol‐3‐ium‐2‐yl)]propane triflate (ADIP), a radical initiator possessing a strong cationic group that is newly developed. By comparing this gel with a PNIPAAm gel prepared using a conventional radical initiator, 2,2′‐azobisisobutyronitrile, the following properties are found: 1) the cationic residue of ADIP is introduced in the gel, and 2) the PNIPAAm gel prepared using ADIP exhibits a discontinuous volume transition as a function of temperature and rapid shrinking in response to temperature jump. This thermoresponsive behavior is attributed to the low polymer fraction in the gel prepared using ADIP. The ADIP‐based design of the thermoresponsive hydrogel investigated herein may contribute to the design of functional soft materials.

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Section: Resultsmentioning

confidence: 99%

Strong Cationic Radical Initiator‐Based Design of a Thermoresponsive Hydrogel Showing Drastic Volume Transition

Masuda

Tsuji

Koizumi

et al. 2020

Macro Chemistry & Physics

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“…Suzuki's group calculated that the whole cell temperature can be increased only by 10 μK when the sarco-/endoplasmic reticulum Ca 2+ -ATPase, Serca, is solely responsible for the measured temperature changes in HeLa cells upon Ca 2 + shock (Takei et al 2014;Arai et al 2014). Furthermore, the presence of a stable temperature variation over 1 K among cellular compartments has been demonstrated in unstimulated cells using different kinds of luminescence thermometry (Okabe et al 2012;Kiyonaka et al 2013;Hayashi et al 2015;Tanimoto et al 2016;Nakano et al 2017;Uchiyama et al 2015;Uchiyama et al 2018). These measurements require additional considerations on the cellular heat power and make the gap even wider.…”

Section: Five Orders Of Magnitude Disagreement Between Calculations Amentioning

confidence: 99%

The challenge of intracellular temperature

Suzuki

Plakhotnik

2020

Biophys Rev

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This short review begins with a brief introductory summary of luminescence nanothermometry. Current applications of luminescence nanothermometry are introduced in biological contexts. Then, theoretical bases of the "temperature" that luminescence nanothermometry determines are discussed. This argument is followed by the 10 5 gap issue between simple calculation and the measurements reported in literatures. The gap issue is challenged by recent literatures reporting single-cell thermometry using non-luminescent probes, as well as a report that determines the thermal conductivity of a single lipid bilayer using luminescence nanothermometry. In the end, we argue if we can be optimistic about the solution of the 10 5 gap issue.

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“…In recent years, the measurement of intracellular temperature has become one of the main topics of chemistry and biophysics, triggered in part by the development of various thermosensitive fluorescent dye molecules . These dyes exhibit marked changes in their fluorescence intensity and/or fluorescence lifetime in response to the surrounding temperature, which can be used for evaluating and visualizing the temperature inside a cell.…”

Section: Figurementioning

confidence: 99%

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

2020

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We propose a label‐free method for measuring intracellular temperature using a Raman image of a cell in the O−H stretching band. Raman spectra of cultured cells and the medium were first measured at various temperatures using a Raman microscope and the intensity ratio of the two regions of the O−H stretching band was calculated. The intensity ratio varies linearly with temperature in both the medium and cells, and the resulting calibration lines allow simultaneous visualization of both intracellular and extracellular temperatures in a label‐free manner. We applied this method to the measurement of temperature changes after the introduction of FCCP (carbonyl cyanide‐p‐trifluoromethoxyphenylhydrazone) in living cells. We observed a temperature rise in the cytoplasm and succeeded in obtaining an image of the change in intracellular temperature after the FCCP treatment.

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A Cell‐Targeted Non‐Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium‐Containing Cationic Radical Initiator

Cited by 56 publications

References 43 publications

Strong Cationic Radical Initiator‐Based Design of a Thermoresponsive Hydrogel Showing Drastic Volume Transition

Strong Cationic Radical Initiator‐Based Design of a Thermoresponsive Hydrogel Showing Drastic Volume Transition

The challenge of intracellular temperature

Label‐Free Imaging of Intracellular Temperature by Using the O−H Stretching Raman Band of Water

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