A cell viability assessment method based on area-normalized impedance spectrum (ANIS)

Zhang, Rongbiao; Wei, Mingji; Chen, Shuohuan; Li, Guoxiao; Zhang, Fei; Yang, Ning; Huang, Linkui

doi:10.1016/j.bios.2018.03.041

Cited by 24 publications

(23 citation statements)

References 17 publications

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“…The Cell Counting Kit-8 (CCK-8) assay was used to screen the cytotoxic or protective activity of XML in the DOX cardiotoxicity cell model. The cell proliferation [16] was determined with CCK-8 (Dojindo, cat: CK04) according to the manufacturer's instructions. H9c2 cells were seeded into 96-well culture plates at a density of 2 × 10 3 cells per well and grown for 24 h. After the corresponding treatment, 10 μL CCK-8 solution was added to each well of the plate, and then, the plate was incubated for 1 hour in the incubator.…”

Section: Measurement Of H9c2mentioning

confidence: 99%

Xinmailong Attenuates Doxorubicin‐Induced Lysosomal Dysfunction and Oxidative Stress in H9c2 Cells via HO‐1

Jiang

Liu

Xiao

et al. 2021

Oxidative Medicine and Cellular Longevity

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The clinical use of doxorubicin (DOX) is limited by its cardiotoxicity, which is closely associated with oxidative stress. Xinmailong (XML) is a bioactive peptide extracted from American cockroaches, which has been mainly applied to treat chronic heart failure in China. Our previous study showed that XML attenuates DOX-induced oxidative stress. However, the mechanism of XML in DOX-induced cardiotoxicity remains unclear. Heme oxygenase-1 (HO-1), an enzyme that is ubiquitously expressed in all cell types, has been found to take antioxidant effects in many cardiovascular diseases, and its expression is protectively upregulated under DOX treatment. Lysosome and autophagy are closely involved in oxidative stress as well. It is still unknown whether XML could attenuate doxorubicin-induced lysosomal dysfunction and oxidative stress in H9c2 cells via HO-1. Thus, this study was aimed at investigating the involvement of HO-1-mediated lysosomal function and autophagy flux in DOX-induced oxidative stress and cardiotoxicity in H9c2 cells. Our results showed that XML treatment markedly increased cell proliferation and SOD activity, improved lysosomal function, and ameliorated autophagy flux block in DOX-treated H9c2 cells. Furthermore, XML significantly increased HO-1 expression following DOX treatment. Importantly, HO-1-specific inhibitor (Znpp) or HO-1 siRNA could significantly attenuate the protective effects of XML against DOX-induced cell injury, oxidative stress, lysosomal dysfunction, and autophagy flux block. These results suggest that XML protects against DOX-induced cardiotoxicity through HO-1-mediated recovery of lysosomal function and autophagy flux and decreases oxidative stress, providing a novel mechanism responsible for the protection of XML against DOX-induced cardiomyopathy.

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Section: Measurement Of H9c2mentioning

confidence: 99%

Xinmailong Attenuates Doxorubicin‐Induced Lysosomal Dysfunction and Oxidative Stress in H9c2 Cells via HO‐1

Jiang

Liu

Xiao

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Z spores is the impedance difference before and after spore activation, which is caused by the growth of spores during culture. When | Z spores | reaches the maximum value, this frequency f 0 is generally considered to be the most sensitive frequency point for spore impedance measurement, which is optimal resolution (Asphahani et al, 2008; Zhang et al, 2018). The measurement results are shown in Figure 3a, absolute 3 represents the highest level, and absolute 1 represents the lowest level.…”

Section: Methodsmentioning

confidence: 99%

A rapid detection method of early spore viability based on AC impedance measurement

Yang

et al. 2020

J Food Process Engineering

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Rice blast is a destructive disease of rice; it is difficult to detect the pathogenic spore viability in early stage. In this paper, a rapid detection method is proposed; the impedance‐viability model of spores was established by measuring the viability of early rice blast spores and the AC impedance at the most sensitive frequencies. This model is a nice linear model, and the goodness of fit R2 is greater than 0.99. At the same time, there is a positive correlation between the spore impedance‐viability model and the hyphae impedance‐growth speed model measured in practice. The p value is less than 0.05, and the Pearson coefficient is close to 1, which proves the feasibility of this method to detect spore viability. Compared with the traditional experimental culture method, the detection speed of spore viability based on impedance method is greatly improved, which brings convenience for the study of rice blast. Practical Applications The viability of spores is one of the most important indexes to show the degree of rice blast. The early spores of the rice blast look similarly, which makes it hard for the naked eye to distinguish the viability, and makes it difficult for farmers to decide whether to use pesticides in the rice field. The traditional method for the detection of spore viability of rice blast is complicated and needs a long time. In recent years, the method of microbial impedance is put forward, which can provide the information of microbial AC impedance, and has the advantages of fast and nondestructive. The results showed that it is feasible to detect spore viability by impedance technique.

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“…However, in order to further improve the accuracy of cell viability evaluation, an approach based on area-normalized impedance spectroscopy (ANIS) was proposed in our previous work. 36 Besides, the electrode area will largely affect the most sensitive frequency as shown in Figure 8c, causing large errors of cell viability assessment. In fact, the sensing model is usually built as the circuit model consisting of the combination of capacitive and resistive elements.…”

Section: ■ Influence Factors Of Theoretical Modelmentioning

confidence: 99%

“…Besides, choosing of the most sensitive frequency used for evaluating cell events may lead to the inaccuracy of experimental results, for the most sensitive frequency was proven to drift due to the changes of cell adhesion area and electrode area, 28 and the consumption of culture medium. 36 So far, the influencing factors mentioned above have not been reviewed. In summary, Li et al 37 mainly reviewed the geometries of electrodes, design parameters, and the types of equivalent circuit model used for the detection of cell events, such as cell adhesion, proliferation, and micromotion.…”

mentioning

confidence: 99%

How to Choose a Proper Theoretical Analysis Model Based on Cell Adhesion and Nonadhesion Impedance Measurement

Wei

Zhang

et al. 2021

ACS Sens.

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The accurate equivalent circuit model contributes to the better fitting of required cell characteristics, such as cell impedance, cell adhesion area, and cell–electrode distance. However, so many theoretical models on specific modules make it difficult for new researchers to understand the whole model of electrode system physically. Besides, the accurate theoretical model and the simplified calculations obviously contradict each other; therefore, it is confusing for many researchers to choose the proper theoretical model to calculate the specific parameters required. In this review, we first discuss the problems and suggestions of electrode system design for cell adhesion-based measurement in terms of parasitic capacitance, detection range of cell number, electric field distribution, and interelectrode distance. The design of electrode system for cell nonadhesion measurement was analyzed in terms of microchannel size and electrode position. Then, we discuss the advantages and disadvantages of various equivalent circuit models according to different requirements of researchers, and simultaneously provide a corresponding theoretical model for researchers. Various factors influencing electric impedance spectroscopy (EIS) such as the parasitic capacitance between microelectrodes, the changes of cell adhesion area and cell–electrode distance, the electrode geometry, and the surface conductivity of electrode were quantitatively analyzed to contribute to better understanding of the equivalent models. Finally, we gave advice to optimize the theoretical models further and perspectives on building uniform principles of theoretical model optimization in the future.

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A cell viability assessment method based on area-normalized impedance spectrum (ANIS)

Cited by 24 publications

References 17 publications

Xinmailong Attenuates Doxorubicin‐Induced Lysosomal Dysfunction and Oxidative Stress in H9c2 Cells via HO‐1

Xinmailong Attenuates Doxorubicin‐Induced Lysosomal Dysfunction and Oxidative Stress in H9c2 Cells via HO‐1

A rapid detection method of early spore viability based on AC impedance measurement

How to Choose a Proper Theoretical Analysis Model Based on Cell Adhesion and Nonadhesion Impedance Measurement

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