A centrifugation-assisted visual detection of SNP in circulating tumor DNA using gold nanoparticles coupled with isothermal amplification

Wang, Yusong; Kong, Say Li

doi:10.1039/c9ra09029k

Cited by 17 publications

(11 citation statements)

References 15 publications

(28 reference statements)

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“…The colorimetric biosensor utilized the amplification property of HCR and high peroxidase with asymmetric catalytic activity to split G-quadruplex DNA zymes, which produces color signals in the presence of ctDNA; this has been successfully applied in complex biological environments such as human blood plasma for ctDNA detection, with the detection limit corresponding to 0.1 pM. Wang et al [ 66 ] developed a rapid centrifugally assisted colorimetric assay using gold nanoparticles (AuNPs) combined with isothermal amplification to detect single nucleotide polymorphisms (SNPs) (G to C mutations) in KRAS and p.G13D in ctDNA. The combination of the two amplification strategies, isothermal amplification and centrifugally assisted assembly, was able to quantitatively and qualitatively distinguish clinically relevant concentrations of approximately 150 nt with a detection limit of 67 pM.…”

Section: Other Ctdna Biosensing Methodsmentioning

confidence: 99%

Electrochemical Biosensors for Circulating Tumor DNA Detection

et al. 2022

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Early diagnosis and treatment have always been highly desired in the fight against cancer, and detection of circulating tumor DNA (ctDNA) has recently been touted as highly promising for early cancer-screening. Consequently, the detection of ctDNA in liquid biopsy is gaining much attention in the field of tumor diagnosis and treatment, which has also attracted research interest from industry. However, it is difficult to achieve low-cost, real-time, and portable measurement of ctDNA in traditional gene-detection technology. Electrochemical biosensors have become a highly promising solution to ctDNA detection due to their unique advantages such as high sensitivity, high specificity, low cost, and good portability. Therefore, this review aims to discuss the latest developments in biosensors for minimally invasive, rapid, and real-time ctDNA detection. Various ctDNA sensors are reviewed with respect to their choices of receptor probes, designs of electrodes, detection strategies, preparation of samples, and figures of merit, sorted by type of electrode surface recognition elements. The development of biosensors for the Internet of Things, point-of-care testing, big data, and big health is analyzed, with a focus on their portable, real-time, and non-destructive characteristics.

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Section: Other Ctdna Biosensing Methodsmentioning

confidence: 99%

Electrochemical Biosensors for Circulating Tumor DNA Detection

et al. 2022

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“…As the quest for more sensitive, more accessible, and less time-consuming assays continues, Wang et al worked on improving colorimetric sensors specifically for the detection of single-nucleotide polymorphisms (SNPs) in ctDNA. The detection assay was optimized through various methods to achieve an LOD of 67 pM [ 37 ]. For instance, a critical linker concentration had to be determined and an extra centrifugation step had to be taken to ease the visual detection of KRAS oncogene mutations [ 37 ].…”

Section: Metal Nanoparticle-based Optical Biosensor For Circulatinmentioning

confidence: 99%

“…The detection assay was optimized through various methods to achieve an LOD of 67 pM [ 37 ]. For instance, a critical linker concentration had to be determined and an extra centrifugation step had to be taken to ease the visual detection of KRAS oncogene mutations [ 37 ]. Rauta et al went even further by developing a CTCs detection platform that takes CTCs viability into account [ 36 ].…”

Section: Metal Nanoparticle-based Optical Biosensor For Circulatinmentioning

confidence: 99%

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

2021

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The effectiveness of cancer treatment strongly depends on the early detection of the disease. Currently, the most common diagnostic method, tissue biopsy, takes time and can be damaging to the patient. Circulating cancer biomarkers such as circulating tumor DNA, micro-RNA (miRNA), tumor proteins, exosomes, and circulating tumor cells have repeatedly demonstrated their viability as targets for minimally invasive cancer detection through liquid biopsies. However, among other things, achieving a great sensitivity of detection is still challenging due to the very low concentration of biomarkers in fluid samples. This review will discuss how the recent advances in nanoparticle-based biosensors are overcoming these practical difficulties. This report will be focusing mainly on optical transduction mechanisms of metal nanoparticles (M-NPs), quantum dots (QDs), and upconversion nanoparticles (UCNPs).

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“…Nanoparticles [25]- [39] have various shapes, from spherical to irregular, are less than 1 micron in diameter and can be made of diverse materials, including polymers, metals, semiconductors, and magnetic substances. Gold nanoparticles are popular due to their commercial availability, optoelectronic properties, and the ease of functionalisation with thiol-modified DNA probes via gold-sulphur bonds.…”

Section: Introductionmentioning

confidence: 99%

The Application of Nanotechnology for Quantification of Circulating Tumour DNA in Liquid Biopsies: A Systematic Review

Aquilina

Qian

et al. 2023

IEEE Rev. Biomed. Eng.

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Technologies for quantifying circulating tumour DNA (ctDNA) in liquid biopsies could enable real-time measurements of cancer progression, profoundly impacting patient care. Sequencing methods can be too complex and time-consuming for regular point-of-care monitoring, but nanotechnology offers an alternative, harnessing the unique properties of objects tens to hundreds of nanometres in size. This systematic review was performed to identify all examples of nanotechnology-based ctDNA detection and assess their potential for clinical use. Google Scholar, PubMed, Web of Science, Google Patents, Espacenet and Embase/MEDLINE were searched up to 23rd March 2021. The review identified nanotechnology-based methods for ctDNA detection for which quantitative measures (e.g., limit of detection, LOD) were reported and biologically relevant samples were used. The pre-defined inclusion criteria were met by 66 records. LODs ranged from 10 zM to 50nM. 25 records presented an LOD of 10fM or below.

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A centrifugation-assisted visual detection of SNP in circulating tumor DNA using gold nanoparticles coupled with isothermal amplification

Abstract: A centrifugation-assisted dual signal amplified visual detection of ctDNA SNP (∼150 nt, 1% clinic sensitivity) within 2 hours has been reported.

Cited by 17 publications

References 15 publications

Electrochemical Biosensors for Circulating Tumor DNA Detection

Electrochemical Biosensors for Circulating Tumor DNA Detection

Recent Advancements in Nanoparticle-Based Optical Biosensors for Circulating Cancer Biomarkers

The Application of Nanotechnology for Quantification of Circulating Tumour DNA in Liquid Biopsies: A Systematic Review

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