A cereal centromeric sequence

Aragón-Alcaide, Luis; Miller, Terry E.; Schwarzacher, Trude; Reader, S. M.; Moore, Graham

doi:10.1007/bf02524643

Cited by 186 publications

(67 citation statements)

References 28 publications

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“…These tandemly repeated sequences evolve rapidly and tend to be conserved only in closely related species . A second type of sequence, represented by RCS1, RCH1, RCH2 and RCH3 is a low to middle-copy, interspersed sequence that may be conserved across many genera (Aragon-Alcaide et al 1996;Jiang et al 1996;Dong et al 1998;Miller et al 1998;Cheng et al 2002). RCS1 was present at the centromeres of Z. palustris, whereas the highly repetitive RCS2/CentO sequence was not present in Z. palustris, even under lowstringency Southern conditions (data not shown).…”

Section: Discussionmentioning

confidence: 93%

Comparative genetics at the gene and chromosome levels between rice (Oryza sativa) and wildrice (Zizania palustris)

et al. 2003

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Using comparative genetics, genes, repetitive DNA sequences and chromosomes were studied in the Oryzeae in order to more fully exploit the rice genome sequence data. Of particular focus was Zizania palustris L., n = 15, commonly known as American wildrice. Previous work has shown that rice chromosomes 1, 4 and 9 are duplicated in wildrice. The Adh1 and Adh2 genes were sequenced and, based on phylogenetic analyses, found to be duplicated in wildrice. The majority of the sequence diversity in the Adh sequences was in intron 3, in which were found several MITE insertions. Cytological and molecular approaches were used to analyze the evolution of rDNA and centromeric repetitive sequences in the Oryzeae. In wildrice, copies of the 5S rDNA monomer were found at two loci on two different chromosomes near the centromeres, as in rice. One nucleolar organizer region (NOR) locus was found adjacent to the telomere, as in rice. RCS1, a middle repetitive sequence in rice, was present in all of the centromeres of wildrice. RCS2/CentO, the highly repetitive component of Oryza sativa L. centromeres, was conserved in eight of the Oryza species examined, but was not found in wildrice. Three other middle repetitive centromeric sequences (RCH1, RCH2/CentO and RCH3) were also examined and found to have variable evolutionary patterns between species of Oryza and Zizania.

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Section: Discussionmentioning

confidence: 93%

Comparative genetics at the gene and chromosome levels between rice (Oryza sativa) and wildrice (Zizania palustris)

et al. 2003

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“…Furthermore, the sequences known as centromere-specific repetitive elements, RCS1 (Aragon- Alcaide et al 1996;Dong et al 1998), RCH1, RCH2 and RCH3 , were homologous to a portion of the internal sequences of RIRE7 (Kumekawa et al 2001). We further investigated the distribution mode of LTRs and gag-pol sequences of other rice retrotransposons, RIRE2 (Ohtsubo et al 1999), RIRE3 (Kumekawa et al 1999), RIRE7 (Kumekawa et al 2001) and RIRE8 (Kumekawa et al 1999).…”

Section: Multiple Repetitive Elements Lie In Cen5mentioning

confidence: 99%

“…Aragon-Alcaide et al (1996) and Jiang et al (1996) first reported the CCS1 family repeats and pSau3A9 repeats commonly found in most of…”

Section: Introductionmentioning

confidence: 99%

The centromere composition of multiple repetitive sequences on rice chromosome 5

Nonomura

Kurata

2001

Chromosoma

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The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Strong suppression of genetic recombination, one of the features of a functional centromere, occurred along the contig region. The most remarkable feature of CEN5 is the composition of the multiple repetitive elements. Oryza-specific RCS2 short tandem repeats were clustered along less than 100 kb at one end of the contig. At least 15 copies of the conserved domain of the 1.9 kb RCE1 centromeric repeats, which are similar to the long terminal repeats (LTRs) of gypsy-type retrotransposon RIRE7, were dispersed mainly in 320 kb stretches next to RCS2 tandem clusters. Many copies of the LTR-like sequences of RIRE3 and RIRE8, another gypsy-type retrotransposon, were also found throughout the contig. On the other hand, the gagpol region was less conserved in the contig. These results indicate that the rice centromere is composed of multiple repetitive sequences with the RCS2 tandem cluster probably being situated as the core of a functional centromere of some hundreds of kilobases to megabases in length.

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“…CENP-B proteins are also found in plants ( Barbosa-Cisneros and HerreraEsparza, 2002), suggesting either that the capture of this retroelement gene predates the plant-animal divergence or, possibly, that capture of this or a similar retroelement gene has occurred multiple times. The CRR and CRM retroelement families of rice and maize, respectively, may represent a more indirect case of transposon adoption (Aragon-Alcaide et al, 1996;Jiang et al, 1996;Ananiev et al, 1998;Miller et al, 1998;Presting et al, 1998). These elements are specifically targeted to centromeres, and show significant sequence conservation among their LTRs, even between species, which may signal that the sequences are under selective pressure and that they predate the divergence of rice and maize (Zhong et al, 2002;Sharma and Presting, 2008).…”

Section: Co-option Of a Te Gene By The Hostmentioning

confidence: 99%