A Changeable Lab-on-a-Chip Detector for Marine Nonindigenous Microorganisms in Ship’s Ballast Water

Maw, Myint Myint; Pan, Xinxiang; Peng, Zhen; Wang, Yanjuan; Zhao, L.; Dai, Bowen; Wang, Junsheng

doi:10.3390/mi9010020

Cited by 8 publications

(7 citation statements)

References 34 publications

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“…An open interface is provided in this microfluidic device to allow precise control for the culture environment. However, there are still some challenges yet to be overcomed related to this microdevice, such as the reliability, integration, and maneuverability [20,[26][27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Miniaturized Platform for Individual Coral Polyps Culture and Monitoring

Luo

Zhao

et al. 2020

Micromachines

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Methodologies for coral polyps culture and real-time monitoring are important in investigating the effects of the global environmental changes on coral reefs and marine biology. However, the traditional cultivation method is limited in its ability to provide a rapid and dynamic microenvironment to effectively exchange the chemical substances and simulate the natural environment change. Here, an integrated microdevice with continuous perfusion and temperature-control in the microenvironment was fabricated for dynamic individual coral polyps culture. For a realistic mimicry of the marine ecological environment, we constructed the micro-well based microfluidics platform that created a fluid flow environment with a low shear rate and high substance transfer, and developed a sensitive temperature control system for the long-term culture of individual coral polyps. This miniaturized platform was applied to study the individual coral polyps in response to the temperature change for evaluating the coral death caused by El Nino. The experimental results demonstrated that the microfluidics platform could provide the necessary growth environment for coral polyps as expected so that in turn the biological activity of individual coral polyps can quickly be recovered. The separation between the algae and host polyp cells were observed in the high culture temperature range and the coral polyp metabolism was negatively affected. We believe that our culture platform for individual coral polyps can provide a reliable analytical approach for model and mechanism investigations of coral bleaching and reef conservation.

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Section: Introductionmentioning

confidence: 99%

Miniaturized Platform for Individual Coral Polyps Culture and Monitoring

Luo

Zhao

et al. 2020

Micromachines

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“…Typically, only cells with a size lower than 2–3 μm are poorly detected in the channel and are often below the detection limit of a microfluidic flow cytometers. To optimize the detection of variable cell sizes, Maw et al , proposed to fit the size of the microfluidic channel in the detection area to the target size [ 22 ]. This system recognizes cells from small bacteria ( Escherichia coli or Enterococcus faecalis , 1–1.5 μm) to microalgae ( Platymonas subcordiformis , 20 μm).…”

Section: Analytical Microfluidic Platformmentioning

confidence: 99%

Microfluidic technology for plankton research

Girault

Beneyton

Amo

2019

Current Opinion in Biotechnology

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Plankton produces numerous chemical compounds used in cosmetics and functional foods. They also play a key role in the carbon budget on the Earth. In a context of global change, it becomes important to understand the physiological response of these microorganisms to changing environmental conditions. Their adaptations and the response to specific environmental conditions are often restricted to a few active cells or individuals in large populations. Using analytical capabilities at the subnanoliter scale, microfluidic technology has also demonstrated a high potential in biological assays. Here, we review recent advances in microfluidic technologies to overcome the current challenges in high content analysis both at population and the single cell level.

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“…The total change of resistance at the detection zone is achieved as the change of voltage at two ends detection channels by our system design. The value of total voltage changes can be calculated with the equation (1) [39,40].…”

Section: Design and Working Principle Of Mrps Detection Systemsmentioning

confidence: 99%