A characterized ethanol extract of Rosa rugosa inhibits hepatic stellate cell activation through elevating Hint1 and subsequent upregulation of Smad7

Ethanol Extract of Rosa rugosa Ameliorates Acetaminophen-Induced Liver Injury via Upregulating Sirt1 and Subsequent Potentiation of LKB1/AMPK/Nrf2 Cascade in Hepatocytes

“…Five active polyphenols, including gallic acid, Di-O-galloyl-HHDP-glucoside, quercetin-3-O-sophoroside, kaempferol-3-O-sophoroside, and ellagic acid, were identified in ERS and analyzed quantitatively using mixed standards of these compounds as references (Figure 1). Data of our chemical composition exploration are comparable to those of previous reports [40][41][42], indicating the reliability of the process to prepare and analyze ERS.…”

Ergothioneine suppresses hepatic stellate cell activation via promoting Foxa3-dependent potentiation of the Hint1/Smad7 cascade and improves CCl4-induced liver fibrosis in mice

“…The knockdown efficiency of this siRNA has been confirmed in our previous study. 12 In LX-2 HSCs, knocking down Hint1 expression abolished EGTmediated elevation of Smad7 (Fig. 3A), and EGT-induced inhibition of their activation triggered by TGFβ1 was also eliminated (Fig.…”

“…point is consistent with previous reports. 12,25,47,49 In LX-2 and primary HSCs, EGT increased Smad7 and inhibited their activation. The above findings, including ours, suggest that upregulation of Smad7 in HSCs can be a feasible strategy for repressing HSC activation and improving LF.…”

“…11 Our recent work unveiled that the expression of Smad7 can be upregulated by Hint1, and increased Hint1 reduces HSC activation and mitigates LF. 12 These findings imply that potentiation of the Hint1/Smad7 cascade is conducive to the repression of HSC activation.…”

“…Sequences for si-Hint1 and si-Con have been reported previously. 12 The sequences for si-Foxa3 are as follows (5′ to 3′): sense: GCC CUA CAA CUU CAA CCA CCC; antisense: GGG UGG UUG AAG UUG UAG GGC.…”