In this study, we have sought to identify the nature of the response to both moderately high (25-32°C) and very high (>32"C) temperatures by examining their interactive effects on the accumulation of functionally important proteins during grain-filling. In particular, we wished to determine if the deleterious effects of very high temperature could be alleviated by subsequently cool conditions. To this end, wheat cv. Oxley was exposed to either 21/16 or 40116°C (daylnight) from 15 to 19 days after anthesis and subsequently grown under one of three temperature regimes until maturity: 21/16 (control), 27/22 or 30125°C. Grain samples were taken throughout grain growth and analysed for protein content and composition. Wheat proteins were separated and quantified as albumin,globulin, monomer, SDS-soluble polymer and SDS-insoluble polymer using size-exclusion high-performance liquid chromatography.The various protein fractions responded differentially and usually additively to moderately high and very high temperatures during grain-filling. Cool post-shock conditions did not alleviate the effects of very high temperature on grain protein composition. Heat treatments reduced po1ymer:monomer ratio because the accumulation of monomer was reduced less than that of polymer. 6). Interpretation of grain quality results from wheat variety trials with reference to high temperature stress. . Possible role for the Glu-Dl locus with respect to tolerance to dough-quality change after heat stress. . The relationship between grain-protein content of wheat and barley and temperatures during grain filling. Australian Journal of Plant Physiology 21, 869-873. Doekes, G.J., and Wennekes, L.M.J. (1982). Effect of nitrogen fertilization on quantity and composition of wheat flour proteins. Cereal Chemistry 59, 276-278.