A Chick Embryo Model for Metastatic Human Prostate Cancer

Kobayashi, Tadahiro; Kawakami, Kiyoshi; Endo, Yuichi; Imao, Tetsuya; Uchibayashi, Tadao; Sasaki, Takuma; Namiki, Mikio

doi:10.1159/000019702

Cited by 27 publications

(21 citation statements)

References 12 publications

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“…This makes this assay suitable to test a batch of similar tumors for comparison of anticancer agents. Such results had already been obtained by other groups for prostate cancer, 18 glioblastoma multiforme, 16 melanoma 13 and leukemia 17 cell lines. Compared with the rodent model, the CAM assay is much faster, as tumors grow on the CAM in several days, whereas it may take weeks to observe subcutaneous xenografts on immunodeficient mice.…”

Section: Discussionsupporting

confidence: 76%

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The chicken chorioallantoic membrane tumor assay as model for qualitative testing of oncolytic adenoviruses

et al. 2011

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Oncolytic adenoviruses are promising anticancer agents. To study and optimize their tumor-killing potency, genuine tumor models are required. Here we describe the use of the chicken chorioallantoic membrane (CAM) tumor model in studies on oncolytic adenoviral vectors. Suspensions of human melanoma, colorectal carcinoma and glioblastoma multiforme cell lines were grafted on the CAM of embryonated chicken eggs. All cell lines tested formed 5-10 mm size tumors, which recapitulated hallmarks of corresponding human specimens. Furthermore, melanoma tumors were injected with adenoviral vector-carrying gene encoding the fusion protein of parainfluenza virus type 5. This led to the induction of cell fusion and syncytia formation in the infected cells. At 6 days post-injection, histological and immunohistochemical analyses of tumor sections confirmed adenovirus replication and syncytia formation. These results demonstrate that the CAM model allows rapid assessment of oncolytic viruses in threedimensional tumors. Hence, this model constitutes an easy and affordable system for preclinical characterization of viral oncolytic agents that may precede the mandatory process of animal testing. Application of this model will help reducing the use of human xenografts in mice for preclinical evaluation of oncolytic viruses and other anticancer agents.

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Section: Discussionsupporting

confidence: 76%

“…Especially, the model is very attractive to study the steps involved in metastasis formation. [12][13][14][15] In the field of cancer therapy, the CAM tumor model has been used to evaluate the efficiency of anticancer drugs, [16][17][18] but has never been used so far for testing oncolytic vectors.…”

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confidence: 99%

The chicken chorioallantoic membrane tumor assay as model for qualitative testing of oncolytic adenoviruses

et al. 2011

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“…Estudios preclínicos demuestran que este fár-maco puede tener actividad frente al cáncer de próstata, objetivándose en un modelo animal con líneas celulares de cáncer de próstata hormonoresistente 42 que la suramina tiene un efecto significativo sobre las metástasis hepáticas pero no sobre las óseas, donde el flujo sanguíneo es menor. Sin embargo, la respuesta antitumoral sólo se logra cuando se mantienen unos niveles en plasma superiores a 250 microgramos/mililitro, lo que constituye un importante problema si consideramos que la toxicidad limitante de dosis, principalmente la neurológica, se encuentra directamente relacionada con la duración de la exposición al fármaco y con niveles plasmáticos mantenidos de 300 microgramos/mililitro o más.…”

Section: Inhibidores De Los Factores De Crecimientounclassified

Tratamiento multidisciplinario actual del cáncer de próstata metastásico

Cordón

Albiach

2003

Actas Urol Esp

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El cáncer de próstata es una causa común de muerte en hombres occidentales. La morbimortalidad asociada suele ser consecuencia directa de la diseminación ósea, presente en hasta un 50% de los pacientes en el momento del diagnóstico. El objetivo del tratamiento de estos enfermos metastá-sicos es la prevención o paliación de los síntomas y de las complicaciones. El arsenal terapéutico es amplio e incluye hormonoterapia, radioterapia externa, radiofármacos, cirugía, quimioterapia, bisfosfonatos y nuevos fármacos (inhibidores de la angiogénesis, inmunoterapia y fármacos que actú-an sobre la diferenciación celular). En la elección del tratamiento se deben considerar múltiples factores: la condición de andrógeno-dependiente o andrógeno-independiente, ya que la terapia hormonal constituye el primer escalón del tratamiento; la localización y extensión de la destrucción ósea; la gravedad del cuadro; la disponibilidad de las diferentes terapias; el estado del paciente; el pronóstico de supervivencia y la relación coste-efecto. Las indicaciones de algunas de estas terapias están claramente establecidas mientras que otras están todavía en estudio para determinar su eficacia, la pauta de tratamiento y las indicaciones. En este artículo se realiza una revisión y actualización de dichos tratamientos.PALABRAS CLAVE: Próstata. Metastásico. Hormonoterapia. Radioterapia. Quimioterapia. Bisfosfonatos. Radiofármacos. Cirugía.Nuevas terapias. ABSTRACTCURRENT MULTIAPPROACH TREATMENT OF METASTATIC PROSTATE CANCER Prostate cancer is one of the commonest causes of cancer-related death in the western world. The morbi-mortality associated is usually a direct consequence of metastatic spread to bone, in up to 50% of patients at first presentation. The aim of treatment of metastatic patients is to alleviate and to prevent the distressing symptoms. The approach include hormone-therapy, radiotherapy, radionuclides, surgery, chemotherapy, bisphosphonates and new drugs (agents that inhibit angiogenesis, inmmunotherapy and therapies that affect the differentiation). Decisions about therapy must also take into consideration the androgen-dependent or independent, so hormone-therapy is the first step of the treatment; the number and location of bone metastases; the severity of symptoms; the available of therapies; the status performance of patient; the prognosis and the cost-effect relationship. Some treatments have established indications whereas others are still in process of study in order to determinate their efficacy, their model of treatment and their indications. This article revises and updates these treatments.

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“…The chorioallantoic membrane (CAM) is a well-established system to study tumor-dependent angiogenesis [20][21][22] . When solid tumors are grafted to the CAM, they display many characteristics of cancers in vivo, including proliferation, invasion, angiogenesis and metastasis [23][24][25][26][27] . Based on the previous experience of our group with CAM xenograft models 20,26,27 , a human MM model was established that combines the advantage of a human 3D culture system with the model of ex ovo developing chicken embryos.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

Martowicz

Kern

Gunsilius

et al. 2015

JoVE

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Multiple myeloma (MM), a malignant plasma cell disease, remains incurable and novel drugs are required to improve the prognosis of patients. Due to the lack of the bone microenvironment and auto/paracrine growth factors human MM cells are difficult to cultivate. Therefore, there is an urgent need to establish proper in vitro and in vivo culture systems to study the action of novel therapeutics on human MM cells. Here we present a model to grow human multiple myeloma cells in a complex 3D environment in vitro and in vivo. MM cell lines OPM-2 and RPMI-8226 were transfected to express the transgene GFP and were cultivated in the presence of human mesenchymal cells and collagen type-I matrix as three-dimensional spheroids. In addition, spheroids were grafted on the chorioallantoic membrane (CAM) of chicken embryos and tumor growth was monitored by stereo fluorescence microscopy. Both models allow the study of novel therapeutic drugs in a complex 3D environment and the quantification of the tumor cell mass after homogenization of grafts in a transgene-specific GFP-ELISA. Moreover, angiogenic responses of the host and invasion of tumor cells into the subjacent host tissue can be monitored daily by a stereo microscope and analyzed by immunohistochemical staining against human tumor cells (Ki-67, CD138, Vimentin) or host mural cells covering blood vessels (desmin/ASMA).In conclusion, the onplant system allows studying MM cell growth and angiogenesis in a complex 3D environment and enables screening for novel therapeutic compounds targeting survival and proliferation of MM cells. Video LinkThe video component of this article can be found at

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A Chick Embryo Model for Metastatic Human Prostate Cancer

Cited by 27 publications

References 12 publications

The chicken chorioallantoic membrane tumor assay as model for qualitative testing of oncolytic adenoviruses

The chicken chorioallantoic membrane tumor assay as model for qualitative testing of oncolytic adenoviruses

Tratamiento multidisciplinario actual del cáncer de próstata metastásico

Establishment of a Human Multiple Myeloma Xenograft Model in the Chicken to Study Tumor Growth, Invasion and Angiogenesis

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