A Chick Embryo Technic for Intravenous and Chemotherapeutic Studies.

Lee, Henry F.; Stavitsky, Abram B.; Lee, Margaret P.

doi:10.3181/00379727-61-15254

Cited by 9 publications

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“…Testing of 4-(imidazolidone-2) caproic acid in the chick embryo. Because of the limited supply of the most active drug, 4-(imidazolidone-2) caproic acid, the intravenous injection of chick embryos (Lee et al, 1946) rather than the guinea pig method was employed. Since the H27Rv strain produced inconsistent results, the A-27 strain of Mycobacterium tuberculosis (14 days on the Proskauer-Beck medium) in doses of 107 bacilli was used.…”

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Growth Inhibition of Tubercle Bacilli by Analogues of Biotin

Pope

1952

J Bacteriol

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Woods' report in 1940 on the mode of action of sulfanilamide initiated a new era in the study of chemotherapeutic agents. In the past decade hundreds of new drugs patterned after specific metabolites have been synthesized. Among these are a number of biotin analogues whose inhibitory activity for a variety of organisms has been described. In most instances activity of the compound has been limited to nonpathogenic organsms requiring an exogenous source of the vitamin, but biotin analogues have shown little activity against organisms that synthesize their own biotin. The present paper reports the inhibitory activity of several biotin antagonists on a virulent strain of Mycobacterium tuberculosis. Our interest in these compounds lies not only in their potential chemotherapeutic value but also in their use in studies of biotin metabolism in the tubercle bacillus. MATERIALS AND METHODS A. In vitro studies. The virulent H37Rv strain of Mycobacterium tuberculovsi was used in this study. Test cultures were made in Proskauer-Beck synthetic medium in 4-oz prescription bottles containing the analogue up to a final volume of 20 ml. One loopful of organisms from a young culture (2 to 4 weeks old) was used as the inoculum and was floated on the surface of the medium. The micro

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confidence: 99%