A Chinese Herbal Decoction, Danggui Buxue Tang,  Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation

Choi, Roy Chi Yan; Gao, Qiufeng; Cheung, Anna Wing Han; Zhu, Judy Ting Ting; Lau, Faye T.C.; Li, Jun; Li, Winnie Z.M.; Chu, Glanice K.Y.; Duan, Ran; Cheung, J.K.H.; Ding, Anwei; Zhao, Kui; Dong, Tina Ting Xia; Tsim, Karl Wah Keung

doi:10.1093/ecam/nen085

Cited by 57 publications

(57 citation statements)

References 38 publications

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“…Cell Culture-Rat primary osteoblasts were cultured and prepared by the method previously described (18) with minor modifications (19). In brief, postnatal day 1 rats were decapitated to collect calvarias.…”

Section: Methodsmentioning

confidence: 99%

Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts

Guo

Choi

Cheung

et al. 2011

Journal of Biological Chemistry

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Flavonoids, a group of natural compounds found in a variety of vegetables and herbal medicines, have been intensively reported on regarding their estrogen-like activities and particularly their ability to affect bone metabolism. Here, different subclasses of flavonoids were screened for their osteogenic properties by measuring alkaline phosphatase activity in cultured rat osteoblasts. The flavone baicalin derived mainly from the roots of Scutellaria baicalensis showed the strongest induction of alkaline phosphatase activity. In cultured osteoblasts, application of baicalin increased significantly the osteoblastic mineralization and the levels of mRNAs encoding the bone differentiation markers, including osteonectin, osteocalcin, and collagen type 1␣1. Interestingly, the osteogenic effect of baicalin was not mediated by its estrogenic activity. In contrast, baicalin promoted osteoblastic differentiation via the activation of the Wnt/ ␤-catenin signaling pathway; the activation resulted in the phosphorylation of glycogen synthase kinase 3␤ and, subsequently, induced the nuclear accumulation of the ␤-catenin, leading to the transcription activation of Wnt-targeted genes for osteogenesis. The baicalin-induced osteogenic effects were fully abolished by DKK-1, a blocker of Wnt/␤-catenin receptor. Moreover, baicalin also enhanced the mRNA expression of osteoprotegerin, which could regulate indirectly the activation of osteoclasts. Taken together, our results suggested that baicalin could act via Wnt/␤-catenin signaling to promote osteoblastic differentiation. The osteogenic flavonoids could be very useful in finding potential drugs, or food supplements, for treating post-menopausal osteoporosis.

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Section: Methodsmentioning

confidence: 99%

Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts

Guo

Choi

Cheung

et al. 2011

Journal of Biological Chemistry

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“…Typhimurium ATCC 14 028, according to research conducted by Nurmahani et al (2012) which demonstrates the inhibition of pathogenic bacteria S. thypii. Phenolic compounds in the extracts of pomegranate peel studied by Choi et al (2011) were able to inhibit the growth of Salmonella. The B. cereus and E. coli ATCC 25 922 was also inhibited by red dragon fruit peel extracts.…”

Section: Characteristics Of Red Dragon Fruit Peel Extractsmentioning

confidence: 98%

Effectiveness of the Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as the Colorant, Antioxidant, and Antimicrobial on Beef Sausage

Manihuruk¹,

Suryati²,

Arief³

2017

Med Pet

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This study aimed to evaluate the effectiveness of red dragon fruit (Hylocereus polyrhizus) peel extracts addition on beef sausages. Red dragon fruit peel extracts were obtained by maceration using solvent at pH 5. Phytochemical characteristics, total phenols, antioxidant, and antimicrobial activity of the peel extracts were observed. Antioxidant and antimicrobial activities of the extracts were associated with high phytochemical compounds and total phenols contained in the extracts. Red dragon fruit peel extracts with various percentages (0%, 20%, 30%, and 40%) were added on beef sausages, and their physicochemical characteristics, nutrients, antioxidant activity, and microbiological profile were analyzed. The data were analyzed using analysis of variance and Duncan's multiple range test. Results showed that the addition of red dragon fruit peel extracts significantly reduced texture values, but increased intensity of luminosity, intensity of red color, and intensity of yellow color (P<0.05) beef sausages. It could be concluded that red dragon fruit peel extract containing phytochemical compounds was effective as an antibacterial agent and natural antioxidant. The addition of red dragon fruit peel extracts was effective in increasing the antioxidant activity and decreasing TBARS values. The addition of red dragon fruit peel extract did not affect the reddish colorization of beef sausages, but it was capable of increasing the yellowish colorization on beef sausage.Keywords: antimicrobial, antioxidant, beef sausage, red dragon fruit peel extract ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efektivitas penambahan ekstrak kulit buah naga merah (Hylocereus polyrhizus) pada sosis daging sapi. Ekstrak kulit buah naga merah dihasilkan dengan maserasi menggunakan pelarut pH 5, dan dilakukan pengamatan karakteristik fitokimia, total fenol, aktivitas antioksidan, dan antimikrob. Hasil analisis menunjukkan bahwa ekstrak kulit buah naga merah memiliki aktivitas antioksidan dan antimikrob alami karena memiliki kandungan senyawa fitokimia dan total fenol dalam ekstrak yang tinggi. Ekstrak kulit buah naga merah dengan persentase berbeda (0%, 20%, 30%, dan 40%) ditambahkan pada pembuatan sosis, dan dilakukan pengamatan karakteristik fisikokimia, zat gizi, aktivitas antioksidan, dan mikrobiologi. Data diolah dengan analisis ragam (ANOVA) dan dilanjutkan uji perbandingan berganda menggunakan uji Duncan. Hasil analisis menunjukkan bahwa penambahan ekstrak kulit buah naga merah menurunkan nilai tekstur, tetapi meningkatkan intensitas kecerahan, intensitas warna merah, dan intensitas warna kuning secara signifikan (P<0.05) sosis daging sapi. Dapat disimpulkan bahwa ekstrak kulit buah naga merah yang mengandung komponen fisikokimia, efektif sebagai antimikroba dan antioksidan alami. Penambahan ekstrak kulit buah naga merah mampu meningkatkan aktivitas antioksidan, dan menurunkan nilai thiobarbituric reactive substance (TBARS). Penambahan ekstrak kulit buah naga merah belum efektif meningkatkan intensitas warna merah sos...

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“…Arg (Sigma Chemical Co.) was also dissolved in DW, filtered with 0.22 mm syringe filter, prepared at a dose of 10 mg/ml according to a previous report (Han et al 2013). 17b-estradiol (E 2, Sigma Chemical Co.) was dissolved in dimethyl sulfoxide, filtered with 0.22 mm syringe filter, prepared at a dose of 100 nM according to a previous report (Choi et al 2011).…”

Section: Preparation Of Ppe Arg and 17b-estradiolmentioning

confidence: 99%

Protective effect of porcine placenta in a menopausal ovariectomized mouse

et al. 2015

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Menopause is a significant physiological phase that occurs as women's ovaries stop producing ovum and the production of estrogen declines. Human placenta and some amino acids are known to improve menopausal symptoms. In this study, we investigated that porcine placenta extract (PPE) and arginine (Arg), a main amino acid of PPE, would have estrogenic activities in ovariectomized (OVX) mice as a menopause mouse model, human breast cancer cell line (MCF-7) cells, and human osteoblast cell line (MG-63) cells. PPE or Arg significantly inhibited the body weight and increased the vagina weight compared to the OVX mice. PPE or Arg ameliorated the vaginal atrophy in the OVX mice. The levels of 17b-estradiol and the activities of alkaline phosphatase (ALP) were significantly increased by PPE or Arg in the serum of OVX mice. Trabecular bone parameters such as bone mineral density and porosity were also improved by PPE or Arg in the OVX mice. In the MCF-7 and MG-63 cells, PPE or Arg significantly increased the cell proliferation, estrogen receptor b mRNA expression, and estrogen-response elements luciferase activity. Finally, PPE or Arg increased the activations of ALP and extracellular signalregulated kinase 1/2 in the MG-63 cells. These results indicate that PPE or Arg would have estrogenic and osteoblastic activity. Therefore, PPE or Arg may be useful as new pharmacological tools for treating menopausal symptoms including osteoporosis. Free Korean abstract: A Korean translation of this abstract is freely available at

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A Chinese Herbal Decoction, Danggui Buxue Tang, Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation

Cited by 57 publications

References 38 publications

Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts

Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts

Effectiveness of the Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as the Colorant, Antioxidant, and Antimicrobial on Beef Sausage

Protective effect of porcine placenta in a menopausal ovariectomized mouse

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