A Chinese herbal prescription Yiqi Jiedu decoction attenuates irradiation induced testis injury in mice

Wang, An; Wang, Lei; Lu, Xi; Wang, Yan; Chen, Xiaoying; Shi, Zhongyu; Li, Panfei; He, Changhao; Zhang, Shujing; Gao, Yue; Wang, Tieshan; Hu, Shengdi

doi:10.1016/j.biopha.2019.109804

Cited by 14 publications

(5 citation statements)

References 48 publications

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“…The use of chemical compounds in radiation therapy is an obvious strategy for improving therapeutic outcomes [ 11 ]. However, due to their inherent toxicity and high cost, most studied synthetic radioactive protective compounds are underutilized clinically [ 12 ]. Therefore, for radiation testicular injury, there is an urgent need for highly effective radiation protective agents with fewer side effects.…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane attenuates irradiation induced testis injury in mice

Ran,

Duan,

Gao

et al. 2023

Redox Report

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Objective: The testis is vulnerable to ionizing radiation, sexual dysfunction and male infertility are common problems after local radiation or whole-body exposure. Currently, there are no approved drugs for the prevention or treatment of radiation testicular injury. Sulforaphane (SFN) is an indirect antioxidant that induces phase II detoxification enzymes and antioxidant genes. Herein, we investigated the radiation protective effect of SFN on testicular injury in mice and its potential mechanism. Materials and Methods: Mice were randomly divided into blank control group (Ctrl), radiation + no pretreatment group (IR), and radiation + SFN groups (IRS). In the radiation + SFN groups, starting from 72 h before radiation, SFN solution was intraperitoneally injected once a day until they were sacrificed. Mice in the blank control group and the radiation + no pretreatment group were simultaneously injected intraperitoneally with an equal volume of the solvent used to dissolve SFN (PBS with a final concentration of 0.1%DMSO) until they were sacrificed. They were subjected to 6Mev-ray radiation to the lower abdominal testis area (total dose 2Gy). Twenty-four hours after radiation, six mice in each group were randomly sacrificed. Seventy-two hours after radiation, the remaining mice were sacrificed. Results: The results showed that the harmful effects of ionizing radiation on testes were manifested as damage to histoarchitecture, increased oxidative stress, and apoptosis, and thus impaired male fertility. SFN injections can reverse these symptoms. Conclusions: The results showed that SFN can improve the damage of mouse testis caused by irradiation. Furthermore, SFN prevents spermatogenesis dysfunction caused by ionizing radiation by activating Nrf2 and its downstream antioxidant gene.

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Section: Introductionmentioning

confidence: 99%

Sulforaphane attenuates irradiation induced testis injury in mice

Ran,

Duan,

Gao

et al. 2023

Redox Report

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“…Hydrogen-rich saline has potential as a novel radiation countermeasure without known toxic side effects; however, its narrow protective time frame (5 min) before radiation has restricted further applications [ 28 ]. Other reported herbal medicines with radioprotective effects need repeated administration [ 29 , 30 ], which may reduce patient compliance and cannot be used in nuclear emergency rescue. Our study clearly shows that a single dose of DMSO within a short period of time before radiation (1 h) is effective at promoting spermatogenesis and reducing the abnormal percentage of sperm even 120 days after exposure.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl Sulfoxide Attenuates Radiation-Induced Testicular Injury through Facilitating DNA Double-Strand Break Repair

Huang

Peng²,

Yu³

et al. 2022

Oxidative Medicine and Cellular Longevity

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The testis is susceptible to ionizing radiation, and male infertility and sexual dysfunction are prevalent problems after whole-body or local radiation exposure. Currently, there is no approved agent for the prevention or treatment of radiation-induced testicular injury. Herein, we investigated the radioprotective effect of dimethyl sulfoxide (DMSO), an organosulfur compound that acts as a free radical scavenger, on testicular injury. Treatment of mice with a single dose of DMSO prior to 5 Gy irradiation restored sex hormones and attenuated the reduction in testis weight. Histological analyses revealed that DMSO alleviated the distorted architecture of seminiferous tubules and promoted seminiferous epithelium regeneration following irradiation. Moreover, DMSO provided quantitative and qualitative protection for sperm and preserved spermatogenesis and fertility in male mice. Mechanistically, DMSO treatment enhanced GFRα-1+ spermatogonial stem cell and c-Kit+ spermatogonial survival and regeneration after radiation. DMSO also alleviated radiation-induced oxidative stress and suppressed radiation-induced germ cell apoptosis in vivo and in vitro. Additionally, DMSO efficiently reduced DNA damage accumulation and induced the expression of phosph-BRCA1, BRCA1, and RAD51 proteins, indicating that DMSO facilitates DNA damage repair with a bias toward homologous recombination. In summary, our findings demonstrate the radioprotective efficacy of DMSO on the male reproductive system, which warrants further studies for future application in the preservation of male fertility during conventional radiotherapy and nuclear accidents.

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“…Unlike the previously published sources entitled "A Chinese herbal prescription Yiqi Jiedu decoction attenuates irradiation induced testis injury in mice" [11], the innovation of this article is mainly reflected in the following aspects. Firstly, different from the previously published articles, the frequency of irradiation is different.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been reported that the main components of herbs in YQJD decoction have a certain antiradiation effect in animal models [8][9][10]. A previous study has shown that YQJD decoction attenuated irradiation-induced testis injury in mice and its potential mechanism was related to the TLR5 signaling pathway on the 7th day after irradiation [11]. Moreover, YQJD decoction could also increase SOD activity [12], reduce the content of TNF-α [13], and alleviate the radiation-induced peripheral blood cytopenia [14].…”

Section: Introductionmentioning

confidence: 97%

Dynamic Protective Effect of Chinese Herbal Prescription, Yiqi Jiedu Decoction, on Testis in Mice with Acute Radiation Injury

Wang

Zhang²,

Yang

2021

Evidence-Based Complementary and Alternative Medicine

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Objective. A Chinese herbal prescription, Yiqi Jiedu (YQJD) decoction, is used for injuries caused by radiotherapy. This study aimed to investigate the dynamic protective effects of YQJD decoction in mice on radiation damage at different time points of the testis. Methods. The 200 male Balb/c mice were randomly divided into 5 groups (blank, model, Anduolin, low-dose YQJD, and high-dose YQJD groups). Blank and model groups were all given stomach deionized water. Anduolin group and high and low dose of Chinese herb groups were treated with Chinese material medical decoction compound for 2 weeks before being irradiated. Except for the blank group, other groups were given whole-body one-time irradiation of 5.5 Gy 60Co gamma rays. Histopathology examination of testes was conducted on the 1st, 7th, 21st, and 35th day after irradiation, respectively. Indexes of sperm motility rate, sperm count, serum levels of testosterone (T), estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were also measured correspondingly. Result. Compared to 1 day and 7 days after irradiation, most of the indexes, including testis index, sperm motility, and count, were decreased on the 21st and 35th day in all irradiated groups. However, compared with the model group, the indexes to assess sperm quality and the morphological structures of testis in low-dose and high-dose YQJD groups were significantly recovered on the 21st and 35th days. Additionally, reproductive hormones, including testosterone and estradiol, were recovered more in low-dose and high-dose YQJD groups on the 21st and 35th days. Conclusion. The YQJD decoction is able to accelerate the recovery of sperm indexes, testosterone, and estradiol levels of sex hormones in mice after irradiation and demonstrated good protective effects on the testis.

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A Chinese herbal prescription Yiqi Jiedu decoction attenuates irradiation induced testis injury in mice

Cited by 14 publications

References 48 publications

Sulforaphane attenuates irradiation induced testis injury in mice

Sulforaphane attenuates irradiation induced testis injury in mice

Dimethyl Sulfoxide Attenuates Radiation-Induced Testicular Injury through Facilitating DNA Double-Strand Break Repair

Dynamic Protective Effect of Chinese Herbal Prescription, Yiqi Jiedu Decoction, on Testis in Mice with Acute Radiation Injury

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