A chloroplast-localized mitochondrial calcium uniporter transduces osmotic stress in Arabidopsis

Teardo, Enrico; Carraretto, Luca; Moscatiello, Roberto; Cortese, Enrico; Vicario, Mattia; Festa, Margherita; Maso, Lorenzo; Bortoli, Sara De; Calì, Tito; Vothknecht, Ute C.; Formentin, Elide; Cendron, Laura; Navazio, Lorella; Szabó, Ildikò

doi:10.1038/s41477-019-0434-8

Cited by 67 publications

(58 citation statements)

References 53 publications

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“…In animal cells the only isoform, MCU (De Stefani et al, 2011;Baughman, 2011) is responsible for Ca 2+ loading into mitochondria, thus helping recovery of resting [Ca 2+ ] cyt . New evidence supports the involvement of MCU isoforms in shaping the organellar Ca 2+ signatures in plants as well Teardo et al, 2017;Selles et al, 2018;Teardo et al, 2019). In particular, cMCU is involved in the generation of the stromal Ca 2+ transient specific for the osmotic stress and mutants lacking cMCU showed an improved drought tolerance (Stael, 2019;Teardo et al, 2019).…”

Section: Current Knowledge Of the Molecular Players Involved In Ca 2+mentioning

confidence: 65%

“…Different abiotic cues, such as cold, oxidative, salt and osmotic stresses were found to evoke stimulus-specific Ca 2+ signals in the chloroplast stroma (Nomura et al, 2012;Sello et al, 2016;Sello et al, 2018;Teardo et al, 2019). Whereas these stimuli were shown to activate Ca 2+ responses in both chloroplasts and nongreen plastids (Sello et al, 2016), the light-to-dark transition was found to elicit a chloroplast-specific response (Sello et al, 2016;Loro et al, 2016).…”

Section: The Emerging Role Of Chloroplast Calcium Signaling In the Trmentioning

confidence: 99%

“…Recently, two proteins belonging to the family of the mitochondrial calcium uniporter (MCU) have been found to mediate Ca 2+ transport across the mitochondria and chloroplast membranes, respectively AtMCU1 (Teardo et al, 2017) and AtMCU6 (later renamed AtcMCU (Teardo et al, 2019). In animal cells the only isoform, MCU (De Stefani et al, 2011;Baughman, 2011) is responsible for Ca 2+ loading into mitochondria, thus helping recovery of resting [Ca 2+ ] cyt .…”

Section: Current Knowledge Of the Molecular Players Involved In Ca 2+mentioning

confidence: 99%

“…Localization prediction was confirmed for AtMCU1/At1g09575 (Teardo et al, 2017), AtMCU2/At1g57610 Selles et al, 2018), AtMCU3/At2g23790 . For AtMCU6 an interesting situation was observed: in tissues harbouring mature chloroplasts, AtMCU6 was efficiently targeted to these photosynthetic organelles, whereas in roots the protein was found in mitochondria (Teardo et al, 2019). Thus, either plastid-specific partners promote targeting of AtMCU6/AtcMCU or targeting depends on the metabolic state of a given cell.…”

Section: Structural and Functional Comparison Between Mcu Isoforms Frmentioning

confidence: 99%

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Chloroplast Calcium Signaling in the Spotlight

et al. 2020

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Calcium has long been known to regulate the metabolism of chloroplasts, concerning both light and carbon reactions of photosynthesis, as well as additional non photosynthesis-related processes. In addition to undergo Ca 2+ regulation, chloroplasts can also influence the overall Ca 2+ signaling pathways of the plant cell. Compelling evidence indicate that chloroplasts can generate specific stromal Ca 2+ signals and contribute to the fine tuning of cytoplasmic Ca 2+ signaling in response to different environmental stimuli. The recent set up of a toolkit of genetically encoded Ca 2+ indicators, targeted to different chloroplast subcompartments (envelope, stroma, thylakoids) has helped to unravel the participation of chloroplasts in intracellular Ca 2+ handling in resting conditions and during signal transduction. Intra-chloroplast Ca 2+ signals have been demonstrated to occur in response to specific environmental stimuli, suggesting a role for these plant-unique organelles in transducing Ca 2+ -mediated stress signals. In this mini-review we present current knowledge of stimulus-specific intrachloroplast Ca 2+ transients, as well as recent advances in the identification and characterization of Ca 2+ -permeable channels/transporters localized at chloroplast membranes. In particular, the potential role played by cMCU, a chloroplast-localized member of the mitochondrial calcium uniporter (MCU) family, as component of plant environmental sensing is discussed in detail, taking into account some specific structural features of cMCU. In summary, the recent molecular identification of some players of chloroplast Ca 2+ signaling has opened new avenues in this rapidly developing field and will hopefully allow a deeper understanding of the role of chloroplasts in shaping physiological responses in plants.

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Section: Current Knowledge Of the Molecular Players Involved In Ca 2+mentioning

confidence: 65%

Section: The Emerging Role Of Chloroplast Calcium Signaling In the Trmentioning

confidence: 99%

Section: Current Knowledge Of the Molecular Players Involved In Ca 2+mentioning

confidence: 99%

Section: Structural and Functional Comparison Between Mcu Isoforms Frmentioning

confidence: 99%

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Chloroplast Calcium Signaling in the Spotlight

et al. 2020

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“…Plants must continuously adapt to adverse environmental conditions, including temperature, salinity, nitrate (NO 3 - ), or drought (Li et al, 2019; Soma et al, 2017; Teardo et al, 2019; Xu et al, 2018; Yang et al, 2019; Zhang et al, 2018). Upon sensing environmental stressors, plants stop growing and trigger protective responses (Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

The stability and ABA import activity of NRT1.2 inArabidopsisare regulated by CEPR2 via phosphorylation modification

Zhang

et al. 2020

Preprint

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ABA signaling and transport play important roles in plant systematic responses to environmental factors. Previously, we reported that C-terminally encoded peptide receptor 2 (CEPR2) balances plant growth and ABA responses by phosphorylating abscisic acid (ABA) receptors. Here, we showed that CEPR2 also directly phosphorylated the ABA transporter NRT1.2 by phosphaproteomics analysis using CEPR2 overexpression line and by physiological and biochemical experiments. Using transgenic seedlings, we demonstrated that NRT1.2 positively regulated the ABA response, and that CEPR2 acted on NRT1.2 epistatically. Specifically, CEPR2 phosphorylated NRT1.2 at serine 292 (Ser292) to promote the degradation of NRT1.2 via the 26S proteasome and vacuolar degradation pathways. However, ABA strongly inhibited the phosphorylation of NRT1.2 at Ser292, stabilizing NRT1.2. Transportation assays in yeast and Xenopus oocytes showed that the nonphosphorylation of NRT1.2 showed high ABA but very low nitrate import activity, while the phosphorylation of NRT1.2 by CEPR2 eliminated NRT1.2-mediated ABA import but slightly increased nitrate transport. These findings were consistent with our analyses of complement mutants in planta. Thus, our results provide detailed mechanisms of NRT1.2 in transporting ABA and nitrate in response to environmental conditions in plants. Besides, it is a better way for plants to overexpress the unphosphorylated NRT1.2 at Ser292 both under high and low nitrate conditions.

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Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

Aksoy,

Yavuz,

Yagiz

et al. 2024

Plant Direct

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GATA is one of the prominent transcription factor families conserved among many organisms in eukaryotes and has different biological roles in many pathways, particularly in light regulation in plants. Although GATA transcription factors (TFs) have been identified in different crop species, their roles in abiotic stress tolerance have not been studied in potato. In this study, we identified 32 GATA TFs in potato (Solanum tuberosum) by in silico analyses, and expression levels of selected six genes were investigated in drought‐tolerant (Sante) and sensitive (Agria) cultivars under light, drought, and combined (light + drought) stress conditions. According to the phylogenetic results, StGATA TFs were divided into four main groups (I, II, III, and IV) and different sub‐groups in I and II (eight and five, respectively). StGATA genes were uniformly localized to each chromosome with a conserved exon/intron structure. The presence of cis‐elements within the StGATA family further supported the possible involvement in abiotic stress tolerance and light response, tissue‐specific expression, and hormonal regulation. Additional PPI investigations showed that these networks, especially for Groups I, II, and IV, play a significant role in response to light and drought stress. Six StGATAs were chosen from these groups for expressional profiling, and their expression in both Sante and Agria was mainly downregulated under purple and red lights, drought, and combined stress (blue + drought and purple + drought). The interactomes of selected StGATAs, StGATA3, StGATA24, and StGATA29 were analyzed, and the accessions with GATA motifs were checked for expression. The results showed that the target proteins, cyclin‐P3‐1, SPX domain‐containing protein 1, mitochondrial calcium uniporter protein 2, mitogen‐activated protein kinase kinase kinase YODA, and splicing factor 3 B subunit 4‐like, mainly play a role in phytochrome‐mediated stomatal patterning, development, and activity. Understanding the interactions between drought stress and the light response mechanisms in potato plants is essential. It will eventually be possible to enhance potato resilience to climate change by manipulating the TFs that play a role in these pathways.

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A chloroplast-localized mitochondrial calcium uniporter transduces osmotic stress in Arabidopsis

Cited by 67 publications

References 53 publications

Chloroplast Calcium Signaling in the Spotlight

Chloroplast Calcium Signaling in the Spotlight

The stability and ABA import activity of NRT1.2 inArabidopsisare regulated by CEPR2 via phosphorylation modification

Genome‐wide characterization and expression analysis of GATA transcription factors under combination of light wavelengths and drought stress in potato

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