A chloroplastic RNA‐binding protein is a new member of the PPR family

Abstract

P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995)

Interactions of Mitochondrial and Nuclear Genes That Affect Male Gametophyte Development

“…HCF152 binds to petB transcripts (Meierhoff et al, 2003). A radish PPR protein of unknown function was identified in a screen for RNA binding proteins, although with which chloroplast RNA(s) it may normally interact is not known (Lahmy et al, 2000). Two animal PPR proteins, human LRP130 and Drosophila BSF, have also been shown to bind RNA (Mancebo et al, 2001;Mili and Pinol-Roma, 2003).…”

Arabidopsis GLUTAMINE-RICH PROTEIN23Is Essential for Early Embryogenesis and Encodes a Novel Nuclear PPR Motif Protein That Interacts with RNA Polymerase II Subunit III

“…In the Arabidopsis genome, >451 PPR genes have been identified and largely annotated with unknown functions (Aubourg et al, 2000;Small and Peeters, 2000;Lurin et al, 2004). Several loss-of-function mutations of the PPR family have been isolated, and they are mostly involved in RNA processing in mitochondria or chloroplasts (Barkan et al, 1994;Lahmy et al, 2000;Hashimoto et al, 2003;Kazama and Toriyama, 2003;Meierhoff et al, 2003;Nakamura et al, 2003;Lurin et al, 2004;Yamazaki et al, 2004;Kotera et al, 2005;Schmitz-Linneweber et al, 2005). Recently, the identification of seven PPR proteins essential for embryogenesis among the predicted EMB gene data set further extended their function in a developmental context (Cushing et al, 2005).…”

“…The role of the PPR motif is still not clear. It would be interesting to know whether the PPR motif itself can bind DNA or RNA, as PPR proteins have been shown to possess DNA or RNA binding activity (Barkan et al, 1994;Ikeda and Gray, 1999;Lahmy et al, 2000;Small and Peeters, 2000;Mancebo et al, 2001;Liu and McKeehan, 2002;Tsuchiya et al, 2002;Meierhoff et al, 2003;Mili and Pinol-Roma, 2003;Nakamura et al, 2003;Williams and Barkan, 2003;Lurin et al, 2004;Schmitz-Linneweber et al, 2005).…”

“…A typical PPR motif, consisting of 35 amino acids, is a macromolecular binding motif that is arranged in tandem with 2 to 26 copies in a PPR protein (Small and Peeters, 2000). Recent genetic and biochemical analysis showed that PPR proteins bind in a sequence-specific manner to RNA or DNA with their PPR motif (Barkan et al, 1994;Ikeda and Gray, 1999;Lahmy et al, 2000;Small and Peeters, 2000;Mancebo et al, 2001;Tsuchiya et al, 2002;Meierhoff et al, 1 These authors contributed equally to this work. 2 To whom correspondence should be addressed.…”

Chloroplast RNA-binding proteins

“…Although some of these factors have been shown to be part of high-molecular-weight complexes which contain their target RNAs, a direct interaction with plastid RNA probes has recently been documented for just one protein, called HCF152, from Arabidopsis thaliana (Meierhoff et al 2003). HCF152 is involved in the processing of transcripts from the chloroplast psbB operon and contains a so-called pentatricopeptide repeat (PPR) motif, which has been hypothesized to form a RNA-binding domain (Lahmy et al 2000;Small and Peeters 2000). Such PPR proteins appear to represent typical plant proteins belonging to a large family of more than 200 members in A. thaliana (Small and Peeters 2000).…”