A chromosomal study ofEumecesandScincus,primitive members of the Scincidae (Reptilia, Squamata)

Caputo, Vincenzo; Odierna, Gaëtano; Aprea, Gennaro

doi:10.1080/11250009409355876

Cited by 23 publications

(33 citation statements)

References 18 publications

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“…on the position of the corresponding peak in the flow karyotype. For this reason these numbers do not completely correspond to those reported by Caputo et al [1994]. Chromosomes 1, 2, 3, 4, 10 and 11 are represented by separate peaks and paints identifying single pairs could be produced from these peaks.…”

Section: Chromosome Paint Productionmentioning

confidence: 78%

“…albifasciatus and S. hemprichii . These species share similar karyotypes with 32-36 metacentric to acrocentric chromosomes, and only the first 2 pairs of biarmed elements are considerably larger than the others [Caputo et al, 1993a[Caputo et al, , 1994Kalboussi et al, 2006]. The aim of the present paper is to investigate, by cross-species chromosome painting, the relationships between these 2 main chromosome complements.…”

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confidence: 91%

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Skinks (Reptilia: Scincidae) Have Highly Conserved Karyotypes as Revealed by Chromosome Painting

Giovannotti

Caputo²,

O’Brien³

et al. 2009

Cytogenet Genome Res

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Skinks represent the most diversified squamate reptiles with a great variation in body size and form, and are found worldwide in a variety of habitats. Their remarkable diversification has been accompanied by only a few chromosome rearrangements, resulting in highly-conservative chromosomal complements of these lizards. In this study cross-species chromosome painting using Scincus scincus (2n = 32) as the source genome, was used to detect the chromosomal rearrangements and homologies between the following skinks: Chalcides chalcides (2n = 28), C. ocellatus (2n = 28), Eumeces schneideri (2n = 32), Lepidothyris fernandi (2n = 30), Mabuya quinquetaeniata (2n = 32). The results of this study confirmed a high degree of chromosome conservation between these species. The main rearrangements in the studied skinks involve chromosomes 3, 5, 6 and 7 of S. scincus. These subtelocentric chromosomes are homologous to the p and q arms of metacentric pair 3 and 4 in C. chalcides, C. ocellatus, L. fernandi, and M. quinquetaeniata, while they are entirely conserved in E. schneideri. Other rearrangements involve S. scincus 11 in L. fernandi and M. quinquetaeniata, supporting the monophyly of Lygosominae, and one of the chromosomes S. scincus 12–16, in M. quinquetaeniata. In conclusion, our data support the monophyly of Scincidae and confirm that Scincus-Eumeces plus Chalcides do not form a monophyletic clade, suggesting that the Scincus-Eumeces clade is basal to other members of this family. This study represents the first time the whole genome of any reptile species has been used for cross-species chromosome painting to assess chromosomal evolution in this group of vertebrates.

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Section: Chromosome Paint Productionmentioning

confidence: 78%

mentioning

confidence: 91%

Skinks (Reptilia: Scincidae) Have Highly Conserved Karyotypes as Revealed by Chromosome Painting

Giovannotti

Caputo²,

O’Brien³

et al. 2009

Cytogenet Genome Res

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“…schneideri suggest that these taxa are to be regarded as chromosomal conservative lineages [see Slamovits and Rossi, 2002 and references therein]. This hypothesis seems to be confirmed by the remarkable karyotype stability that can be observed in E. schneideri and its closely related species E. algeriensis , S. scincus , and S. hemprichii [Caputo et al, 1994].…”

Section: Discussionmentioning

confidence: 94%

“…Eumeces (sensu stricto) comprises 3 species with a Paleartic distribution: E. algeriensis , E. indothalensis , and E. schneideri [Sindaco and Jeremčenko, 2008]. Despite species richness and diversification, Scincidae are karyologically poorly known, with only about 6% of species karyotyped [Caputo et al, 1994] and so far no satDNAs have been isolated from these lizards. The aim of this paper was to isolate and characterise a satDNA in 1 species of this family, E. schneideri , to contribute to a more thorough understanding of the genome composition of these squamates.…”

mentioning

confidence: 99%

Characterisation of a GC-Rich Telomeric Satellite DNA in Eumeces schneideri Daudin (Reptilia, Scincidae)

Giovannotti

Cerioni

Caputo

et al. 2009

Cytogenet Genome Res

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A hitherto undescribed satellite DNA family (AvaII satDNA) has been isolated and characterised in Eumeces schneideri, a squamate reptile belonging to the family Scincidae. AvaII satDNA is characterised by a monomer length of 208 bp, a GC content of 59% and exhibits a certain degree of CpG methylation. FISH experiments with AvaII satDNA probe produced bright signals (i) at the end of the short arms of all subtelocentric chromosomes except for pair 14, in which the signal was at the end of the long arms, (ii) at the ends of both arms of the small metacentric chromosomes 12, and (iii) in a terminal position on the acrocentric chromosomes 11 and 13. AvaII satDNA repeats were not found in the metacentric pair 3, whereas only a weak interstitial signal occurred in the metacentric pairs 1 and 2. C-banding showed that this satellite represents most of the constitutive heterochromatin in the genome of this skink, and chromomycin A₃ staining produced a clear signal overlapping with the satellite, except for NOR-associated heterochromatin. In addition, quantitative dot blot analysis showed that these repetitive sequences constitute about 3% of the genomic DNA of this lizard. AvaII satDNA sequence analysis revealed the occurrence of short guanine residue stretches for which a function in structural stability of these sequences and a role in recombination with telomeric sequences can be hypothesised. Fibre FISH experiments showed that on some chromatin fibres telomeric sequences and AvaII satellite DNA repeats are intermingled or overlapping.

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“…is closer to Scincus than any other E. schneiderii group species. Most studies based on karyological analyses (Kupriyanova, 1986;Caputo et al, 1993Caputo et al, , 1994 and morphological/molecular studies (Carranze et al 2008) showed the paraphyly of E.schneiderii group species and argued that Scincus may be derived from an E. schneiderii-like stock. The Scincus Eumeces clade considered basal to other members of the family (Giovannotti et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of the genus Eumeces Wiegmann, 1834 (Reptilia: Scincidae) in Iran, inferred from 16s mitochondrial DNA

Feizi¹,

Heidari²,

Pouyani³

et al. 2016

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A chromosomal study ofEumecesandScincus,primitive members of the Scincidae (Reptilia, Squamata)

Cited by 23 publications

References 18 publications

Skinks (Reptilia: Scincidae) Have Highly Conserved Karyotypes as Revealed by Chromosome Painting

Skinks (Reptilia: Scincidae) Have Highly Conserved Karyotypes as Revealed by Chromosome Painting

Characterisation of a GC-Rich Telomeric Satellite DNA in <i>Eumeces schneideri</i> Daudin (Reptilia, Scincidae)

Molecular phylogeny of the genus Eumeces Wiegmann, 1834 (Reptilia: Scincidae) in Iran, inferred from 16s mitochondrial DNA

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