A chronic oral exposure of pigs with deoxynivalenol partially prevents the acute effects of lipopolysaccharides on hepatic histopathology and blood clinical chemistry

Stanek, C.; Reinhardt, Nicole; Diesing, Anne-Kathrin; Nossol, Constanze; Kahlert, Stefan; Panther, Patricia; Kluess, Jeannette; Rothkötter, Hermann‐Josef; Kuester, Doerthe; Brosig, Bianca; Kersten, Susanne; Dänicke, Sven

doi:10.1016/j.toxlet.2012.10.009

Cited by 24 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of LPS-induced hepatic necrosis, often triggered by infiltration of neutrophils causing oxidative stress (Ramaiah and Jaeschke 2007) and reported already in endotoxaemic models (Li et al 2012; Depboylu et al 2013), might be explained by the rather short observation period in our study (3 h). However, no additive, synergistic or antagonistic impact of DON feeding on the LPS-induced morphological hepatic changes was detectable in our study, in contrast to earlier investigations reporting a more attenuating impact of dietary DON (Stanek et al 2012). …”

Section: Discussioncontrasting

confidence: 99%

“…This is in accordance with previous studies in young pigs (Stanek et al 2012) and pregnant sows (Tiemann et al 2008), applying oral DON concentrations in the same range as in our present study. In contrast, Gerez et al (2015) observed histological lesions of the liver described as disorganization of hepatic cords, cytoplasmic vacuolization of hepatocytes and focal necrosis after feeding a diet contaminated with DON (1.5 mg/kg feed) or a combination of DON, nivalenol and zearalenone to 5-week-old male piglets.…”

Section: Discussionsupporting

confidence: 94%

“…Severe haemorrhages of the liver were observed after LPS administration, also reflected in the greater relative liver weight as reported previously in dogs (MacLean et al 1956), broilers (Mireles et al 2005) and pigs (Stanek et al 2012). The histopathological examination of liver samples in LPS-infused pigs confirmed the haemorrhage and showed also a marked infiltration of granulocytes in all regions of liver lobules.…”

Section: Discussionsupporting

confidence: 80%

“…Previously, it has been demonstrated that pigs showed an attenuated immune response after challenge with ovalbumin (Grenier et al 2011) or sheep red blood cells (Rotter et al 1994) when previously exposed to dietary Fusarium toxins. Such a “priming effect” of DON was also demonstrated in a porcine endotoxaemic model (Stanek et al 2012), where dietary DON exposure also partially attenuated LPS induced hepatic lesions when compared to control-fed counterparts.…”

Section: Introductionmentioning

confidence: 66%

“…Sections (3–5 μm) were cut on a HM 355S rotation microtom (Microm International GmbH, Germany) and mounted on glass slides (2 sections/slide). Specimens were HE stained and blinded sections (2 sections/sample) were evaluated (brightfield microscopy) using the modified histology activity index (HAI) based on Ishak et al (1995) and modified by Stanek et al (2012). The modified HAI incorporates signs of inflammation (portal, periportal and acinar infiltration of neutrophil and eosinophil granulocytes), necrosis (focal and confluent) and haemorrhages (Fig.…”

Section: Methodsmentioning

confidence: 99%

See 4 more Smart Citations

Chronic DON exposure and acute LPS challenge: effects on porcine liver morphology and function

et al. 2017

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to examine the role of chronic deoxynivalenol (DON) exposition on the liver morphology and function in combination with pre- and post-hepatic lipopolysaccharide (LPS) stress in young pigs fed for 4 weeks with a DON-contaminated diet (4.59 mg/kg feed). At the end of the experiment, LPS (7.5 μg/kg BW) was administered for 1 h pre-hepatically (Vena portae hepatis) or post-hepatically (Vena jugularis). Liver morphology was macroscopically checked and showed haemorrhage in all LPS groups, significantly higher relative liver weights, accompanied by marked oedema in the gallbladder wall. Histological changes were judged by a modified histology activity index (HAI). Liver HAI score was significantly increased in all LPS groups compared to placebo, primarily due to neutrophil infiltration and haemorrhage. DON feed alone was without effect on the liver HAI. Liver function was characterized by (i) hepatic biochemical markers, (ii) mitochondrial respiration and (iii) Ca2+ accumulation capacity of isolated mitochondria. Clinical chemical parameters characterizing liver function were initially (<3 h) slightly influenced by LPS. After 3 h, bilirubin and alkaline phosphatase were increased significantly, in DON-fed, jugular-infused LPS group. Respiration and Ca2+ accumulation capacity of isolated liver mitochondria was not impaired by chronic DON exposure, acute LPS challenge or combined treatments. DON-contaminated feed did not change macroscopy and histology of the liver, but modified the function under LPS stress. The different function was not linked to modifications of liver mitochondria.Electronic supplementary materialThe online version of this article (doi:10.1007/s12550-017-0279-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Chronic DON exposure and acute LPS challenge: effects on porcine liver morphology and function

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

Current sights for mechanisms of deoxynivalenol‐induced hepatotoxicity and prospective views for future scientific research: A mini review

Zhao

Chen

Nüssler

et al. 2016

J of Applied Toxicology

View full text Add to dashboard Cite

Deoxynivalenol (DON) belongs to the group B trichothecenes, which are the most common mycotoxins in cereal commodities. It is very stable within the temperature range 170-350 °C, showing no reduction in its concentration after 30 min at 170 °C. This chemical property is a very dangerous factor for human and animal health. Liver is also responsible for the detoxification and formation of DON-glucuronide in both human and animals. Some studies already demonstrated that DON could induce liver damage remarkably through DON altering expressions of p53, caspase-3, caspase-7, caspase-8 and Bax in different cell lines. At the same time, other publications illustrated some opposite results. At present, a full and systematic discussion of the hepatic toxicity of DON is still lacking. Therefore, the aim of the present review is to summarize and update the prominent evidence, regarding DON effects on liver tissues and cell lines. Moreover, based on the current studies we outline some of the identified molecule targets or pathways involved in DON-induced hepatotoxicity, and put forward our opinions and suggest an hypothesis for future research. Copyright © 2016 John Wiley & Sons, Ltd.

show abstract

Cytochrome P450 enzymes mediated by DNA methylation is involved in deoxynivalenol-induced hepatoxicity in piglets

et al. 2022

View full text Add to dashboard Cite

A chronic oral exposure of pigs with deoxynivalenol partially prevents the acute effects of lipopolysaccharides on hepatic histopathology and blood clinical chemistry

Cited by 24 publications

References 41 publications

Chronic DON exposure and acute LPS challenge: effects on porcine liver morphology and function

Chronic DON exposure and acute LPS challenge: effects on porcine liver morphology and function

Current sights for mechanisms of deoxynivalenol‐induced hepatotoxicity and prospective views for future scientific research: A mini review

Cytochrome P450 enzymes mediated by DNA methylation is involved in deoxynivalenol-induced hepatoxicity in piglets

Contact Info

Product

Resources

About