A chronic, stereotaxic guide-tube platform for intracranial injections in macaques

Dooley, David; Dubach, Mark; P, Blake; Bowden, Douglas M.

doi:10.1016/0165-0270(81)90026-1

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…The design and construction of the platform assembly are a modification of the method of Dooley et al (1981). A custom mold was fabricated (Elmeco Engineering, Rockville, MD) with a total of 12 rows by 14 columns spaced 2 mm apart to maximize the number of sites for injections.…”

Section: Infusion Platformmentioning

confidence: 99%

GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

Wellman¹,

Gale²,

Málková³

2005

J. Neurosci.

122

147

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Amygdala ablation disrupts reinforcer "devaluation" in monkeys (Malkova et al., 1997). Here, we tested the hypothesis that transient inactivation of amygdala by the GABA A agonist muscimol (MUS), specifically during the period of reward satiation, would have a similar effect. Six pigtail macaques were trained on a visual object discrimination task in which 60 objects were associated with one of two specific food rewards. Subsequently, we evaluated the selective satiation-induced change (devaluation) in object preference in probe sessions. We also examined the effect of the amygdala inactivation during the probe sessions to determine whether the inactivation limited to the testing period (and not during the satiation period) is sufficient to impair the expression of reinforcer devaluation. MUS infusions were aimed at basolateral amygdala (BLA) in a pseudorandomized design; each monkey received MUS or saline either before or after selective satiation with each of the two food rewards (six infusions total). Under the control (saline) condition, the monkeys significantly shifted their preference from objects representing the sated food rewards to those representing the nonsated rewards (30% change). When BLA was inactivated during selective satiation (i.e., MUS infused before satiation), this devaluation effect was blocked. In contrast, MUS infusion after satiation, so that it was present just during the testing period, did not impair the shift in object preference (27% change). Thus, BLA is necessary for the appropriate registration of the change in the reinforcer value but not for the subsequent expression of the devaluation involving its transfer to secondary reinforcers.

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Section: Infusion Platformmentioning

confidence: 99%

GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

Wellman¹,

Gale²,

Málková³

2005

J. Neurosci.

122

147

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“…The platform core was precast on a grid of parallel 19-gauge holes containing stainless-steel channels as previously described [Dooley et al, 1981]. In order to permit postimplantation MRI to check the location of guide channels, no metal was used in the platform construction or attachment.…”

Section: Cranial Platformmentioning

confidence: 99%

Development of a Model of Status epilepticus in Pigtailed Macaque Infant Monkeys

Dubach

Szot

et al. 1999

Dev Neurosci

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Seizures, particularly multiple episodes and/or status epilepticus (SE) are prevalent in pediatric patients. Pediatric SE is associated with brain changes that have been hypothesized to contribute to the onset of temporal lobe epilepsy (TLE). In order to gain insight into the effects of seizures on the immature brain and the risk for later TLE, we have developed a model of limbic SE in the pigtailed macaque monkey. In separate studies, bicuculline methiodide or a bicuculline ‘cocktail’ was infused into three regions of the brain (area tempestas, hippocampus, entorhinal cortex) to induce seizures. Measures included MRI, electrophysiology, behavior and morphology. Our results suggest that monkey models of SE may provide useful tools for understanding the effects of prolonged seizures during infancy and the origins of TLE in humans.

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“…Roving electrodes were run along 104 tracks (5–31 tracks per monkey). Details of the surgical preparation for implanting electrodes are described elsewhere (Dooley et al, 1981; Robinson, 1967). Briefly, with the animal under general anesthesia a manually constructed acrylic platform containing multiple electrode guide tubes was positioned stereotaxically, anchored by miniature bolts, and cemented to the calvarium (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Mapping reward mechanisms by intracerebral self‐stimulation in the rhesus monkey (Macaca mulatta)

Bowden

German

2021

J of Comparative Neurology

Self Cite

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The objective of the study was to identify brain structures that mediate reward as evidenced by positive reinforcing effects of stimuli on behavior. Testing by intracerebral self‐stimulation enabled monkeys to inform whether activation of ~2900 sites in 74 structures of 4 sensorimotor pathways and 4 modulatory loop pathways was positive, negative or neutral. Stimulation was rewarding at 30% of sites, negative at 17%, neutral at 52%. Virtually all (99%) structures yielded some positive or negative sites, suggesting a ubiquitous distribution of pathways transmitting valence information. Mapping of sites to structures with dense versus sparse dopaminergic (DA) or noradrenergic (NA) innervation showed that stimulation of DA‐pathways was rewarding or neutral. Stimulation of NA‐pathways was not rewarding. Stimulation of association areas was generally rewarding; stimulation of purely sensory or motor structures was generally negative. Reward related more to structures' sensorimotor function than to density of DA‐innervation. Stimulation of basal ganglia loop pathways was rewarding except in lateral globus pallidus, an inhibitory structure in the negative feedback loop; stimulation of the cerebellar loop was rewarding in anterior vermis and the spinocerebellar pathway; and stimulation of the hippocampal CA1 loop was rewarding. While most positive sites were in the DA reward system, numerous sites in sparsely DA‐innervated posterior cingulate and parietal cortices may represent a separate reward system. DA‐density represents concentrations of plastic synapses that mediate acquisition of new synaptic connections. DA‐sparse areas may represent innate, genetically programmed reward‐associated pathways. Implications of findings in regard to response habituation and addiction are discussed.

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A chronic, stereotaxic guide-tube platform for intracranial injections in macaques

Cited by 7 publications

References 9 publications

GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

Development of a Model of Status epilepticus in Pigtailed Macaque Infant Monkeys

Mapping reward mechanisms by intracerebral self‐stimulation in the rhesus monkey (Macaca mulatta)

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A chronic, stereotaxic guide-tube platform for intracranial injections in macaques

Cited by 7 publications

References 9 publications

GABAA-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

GABAA-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

Development of a Model of Status epilepticus in Pigtailed Macaque Infant Monkeys

Mapping reward mechanisms by intracerebral self‐stimulation in the rhesus monkey (Macaca mulatta)

Contact Info

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GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques

GABA_A-Mediated Inhibition of Basolateral Amygdala Blocks Reward Devaluation in Macaques