A chronic very low dose of gamma-rays alters cell adhesion

Long duration space missions expose astronauts to ionizing radiation events associated with highly energetic and charged heavy particles. Such exposure can result in chromosomal aberrations increasing the likelihood of the development of cancer. Early detection and mitigation of these events is critical in providing positive outcomes. In order to aid in the development of portable devices used to measure radiation exposure, we constructed a genome-wide screen to detect transcriptional changes in peripheral blood lymphocytes shortly after (approximately 1 hour) radiation exposure at low (0.3 Gy), medium (1.5 Gy) and high (3.0 Gy) doses compared to control (0.0 Gy) using Affymetrix® Human Gene 1.0 ST v1 microarrays. Our results indicate a number of sensitive and specific transcriptional profiles induced by radiation exposure that can potentially be implemented as biomarkers for radiation exposure as well as dose effect. For overall immediate radiation exposure, KDELC1, MRPS30, RARS, and HEXIM1 were determined to be effective biomarkers while PRDM9, CHST4, and SLC26A10 were determined to be biomarkers specific to 0.3 Gy exposure; RPH, CCDC96, WDYHV1, and IFNA16 were identified for 1.5 Gy exposure; and CWC15, CHCHD7, and DNAAF2 were determined to be sensitive and specific to 3.0 Gy exposure. The resulting raw and analyzed data are publicly available through NCBI's Gene Expression Ominibus via accession GSE64375.

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Influence of a Continuous Very Low Dose of Gamma-Rays on Cell Proliferation, Apoptosis and Oxidative Stress

Lacoste-Collin

Jozan

Pereda

et al. 2014

Dose-Response

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We have previously shown a delay of death by lymphoma in SJL/J mice irradiated with continuous very low doses of ionizing radiation. In order to understand the mechanisms involved in this phenomenon, we have irradiated in vitro the Raw264.7 monocytic and the YAC-1 lymphoma cell lines at very low-dose rate of 4cGy.month-1. We have observed a transient increase in production of both free radicals and nitric oxide with a transient adaptive response during at least two weeks after the beginning of the irradiation. The slight decrease of Ki67 proliferation index observed during the second and third weeks of YAC-1 cells culture under irradiation was not significant but consistent with the shift of the proliferation assay curves of YAC-1cells at these same durations of culture. These in vitro results were in good agreement with the slightly decrease under irradiation of Ki67 proliferative index evaluated on lymphomatous lymph nodes of SJL/J mice. A significant decrease of YAC-1 cells apoptotic rate under radiation appeared after 4 weeks of culture.Therefore very small doses of gamma-irradiation are able to modify the cellular response. The main observations did not last with increasing time under irradiation, suggesting a transient adaptation of cells or organisms to this level of irradiation.

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A chronic very low dose of gamma-rays alters cell adhesion

Cited by 3 publications

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An innovative in vitro device providing continuous low doses of γ-rays mimicking exposure to the space environment: A dosimetric study

An innovative in vitro device providing continuous low doses of γ-rays mimicking exposure to the space environment: A dosimetric study

Elucidation of dose-dependent transcriptional events immediately following ionizing radiation exposure

Influence of a Continuous Very Low Dose of Gamma-Rays on Cell Proliferation, Apoptosis and Oxidative Stress

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