A cisplatin slow-release hydrogel drug delivery system based on a formulation of the macrocycle cucurbit[7]uril, gelatin and polyvinyl alcohol

Abstract

The anticancer drug cisplatin was encapsulated within the cucurbit [7]uril macrocycle to form the host-guest complex: cisplatin@CB [7]. This was then incorporated into gelatin and 0-4% w/v polyvinyl alcohol (PVA)-based hydrogels as slow release drug delivery vehicles. The hydrogels demonstrated predicable swelling and disintegration dependent on the PVA concentration. The hydrogel with the highest PVA content was slower to swell and release drug compared with lower concentrations of PVA. The effect of the hydrogel PVA concentration on in vitro cytotoxicity was examined using A2780/CP70 ovarian cancer cells. Over the 24 h drug exposure time used, hydrogels containing 4% PVA showed a 20% decrease in viable cells compared to the control, whereas hydrogels containing 0% and 2% PVA induced an 80% and 45% inhibition of cell growth, respectively. There was no measurable difference in the in vitro cytotoxicity of free cisplatin and cisplatin@CB [7] containing hydrogels. Finally, the in vivo effectiveness of a 2%-PVA hydrogel implanted under the skin of nude mice bearing A2780/CP70 xenografts showed that low dose hydrogels containing cisplatin@CB [7] (30 µg equivalent of drug) was just as effective as an intraperitoneal high dose administration of free cisplatin (150 µg) at inhibiting tumour growth.

Cucurbit[n]urils as excipients in pharmaceutical dosage forms

“…Cucurbit [7]uril has been formulated into gelatin-and polyvinyl alcohol (PVA)-based hydrogels (40). When used to form a host-guest complex with the anticancer drug cisplatin, the subcutaneous implantation of the hydrogels meant that they were able to act as a slow release delivery system.…”

“…When used to form a host-guest complex with the anticancer drug cisplatin, the subcutaneous implantation of the hydrogels meant that they were able to act as a slow release delivery system. An in vivo human ovarian cancer model using the hydrogels demonstrated that they were highly effective, with a 2% PVA formulation with cisplatin@CB[7] (10) being just as effective as a 5 x higher dose of free cisplatin delivered via an intraperitoneal injection (40).…”

Low-cost and cleanroom-free fabrication of microneedles

“…Later on, we used the molds to fabricate degradable microneedle patch with polyvinyl alcohol (PVA). PVA is a water-soluble polymer with wide practical application in a variety of fields, including wound dressing, implants, cell encapsulation, drug-delivery systems, soft contact lenses and dental applications, due to its excellent chemical resistance and physical properties, low toxicity and high biocompatibility [14][15][16] . An aqueous solution of PVA can form hydrogels through cast-drying process, where PVA physically cross links during the dehydration process 17 .…”

Rice Husk Char/Poly-(Acrylic Acid-co-acrylamide) Superabsorbent Hydrogels: Preparation, Characterization, and Swelling Behaviors

“…Generally, a superabsorbent can absorb abundant water or other aqueous solutions. Because of superior properties compared to traditional materials such as sponges, they are widely used in hygiene (Kosemund et al 2009), agriculture (Guo et al 2005;Liang et al 2009), drug delivery (Reddy et al 2011;Oun et al 2014), and water purification (Zheng et al 2010;Wang et al 2011). The research on the use of superabsorbents as water and fertilizer managing materials for the renewal desert environment (Banedjschafie and Durner 2015) and slow release fertilizers (Li et al2016;Yamamoto et al 2016) has attracted great attention, and encouraging results have been observed, as they can reduce irrigation water consumption, lower the death rate of plants, and increase plant growth rate (Parvathy et al 2014).…”