2003
DOI: 10.1046/j.1365-2958.2003.03752.x
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A CLC‐type chloride channel gene is required for laccase activity and virulence in Cryptococcus neoformans

Abstract: SummaryLaccase is a major virulence factor required for infection caused by the human pathogenic yeast Cryptococcus neoformans . However, cellular processes involved in the regulation and expression of laccase remain largely unknown in C. neoformans . Here we report the identification of a chloride channel gene CLC-A which is essential for laccase activity in C. neoformans . CLC-A shares homology to CLC-type voltage-gated chloride channels from other organisms; for example, 63% homology to GEF1 , a chloride ch… Show more

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Cited by 76 publications
(87 citation statements)
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References 41 publications
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“…These include genes in cell wall synthesis, metal ion transport and virulence regulators. 29,30 In contrast, our study identifies novel genes that are upregulated in the presence of melanization substrate. Gene induction is only observed in cells capable of melanization, suggesting the genes either have a role in this process or are induced as a consequence of melanization.…”
Section: Methodscontrasting
confidence: 41%
“…These include genes in cell wall synthesis, metal ion transport and virulence regulators. 29,30 In contrast, our study identifies novel genes that are upregulated in the presence of melanization substrate. Gene induction is only observed in cells capable of melanization, suggesting the genes either have a role in this process or are induced as a consequence of melanization.…”
Section: Methodscontrasting
confidence: 41%
“…5G), as well as CLC-A and CPL1, which encode proteins involved in maintaining ion balance (Zhu and Williamson 2003;unpubl.). Deletion of any of these five genes results in a hypocapsular phenotype.…”
Section: Chip-experiments Validate Netprophet Predictionsmentioning
confidence: 99%
“…The fungal strains were grown in YPD medium (2% glucose, 1% yeast extract, 2% Bacto-peptone) or YPD agar medium (YPD and 2% agar). Asparagine minimum selective medium for transformant selection and for detection of laccase production was previously described (22). V8 juice and pigeon guano medium were used for mating assays as described (3,23).…”
Section: Methodsmentioning
confidence: 99%