A “Cleanup Procedure” involving periodate oxidation in the enzymatic synthesis of chemically pure α-32P and α-33P labelled deoxyribonucleotides

Muthukumaran, T.; Krishnamurthy, N.; Sudhaharan, T.; Muralidharan, B.

doi:10.1016/j.apradiso.2005.03.002

Cited by 2 publications

(1 citation statement)

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“…This was classically analyzed. Residual 5′-phosphorylated nucleoside precursors carried over from the incubation were first removed by treating samples with NaIO 4 (10 mM, 30 min, then pinacol to remove excess periodate; Muthukumaran et al , 2005 ). The materials were then digested with concentrated ammonia (see, e.g.…”

Section: Resultsmentioning

confidence: 99%

Catalytic Synthesis of Polyribonucleic Acid on Prebiotic Rock Glasses

et al. 2022

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Reported here are experiments that show that ribonucleoside triphosphates are converted to polyribonucleic acid when incubated with rock glasses similar to those likely present 4.3–4.4 billion years ago on the Hadean Earth surface, where they were formed by impacts and volcanism. This polyribonucleic acid averages 100–300 nucleotides in length, with a substantial fraction of 3′,-5′-dinucleotide linkages. Chemical analyses, including classical methods that were used to prove the structure of natural RNA, establish a polyribonucleic acid structure for these products. The polyribonucleic acid accumulated and was stable for months, with a synthesis rate of 2 × 10 −3 pmoles of triphosphate polymerized each hour per gram of glass (25°C, pH 7.5). These results suggest that polyribonucleotides were available to Hadean environments if triphosphates were. As many proposals are emerging describing how triphosphates might have been made on the Hadean Earth, the process observed here offers an important missing step in models for the prebiotic synthesis of RNA.

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Section: Resultsmentioning

confidence: 99%