A clinical evaluation of trichomoniasis in San Jose, Costa Rica using the InPouch TV test.

Abstract

OBJECTIVE--to determine the prevalence of trichomoniasis in San Jose, Costa Rica, comparing two methods, the InPouch TV test and the saline wet mount. METHODS--One hundred symptomatic and asymptomatic female patients at two hospitals and at a sexually transmitted disease (STD) clinic were evaluated. Vaginal discharge was the most prevalent genitourinary abnormality among symptomatic patients. The patients were between 18 and 70 years old. Fifty-seven were from the STD clinic, 43 from the two hospitals. A saline wet mount and a culture were taken from each patient. The culture employed a new procedure for diagnosis of trichomonads, the InPouch TV test (BioMed Diagnostics, San Jose, CA). RESULTS--Thirteen of the 100 patients were culture positive, two of whom were wet mount positive. No wet mount positives were culture negative. Eleven of the positive tests were from the STD clinic and two were from the hospitals. CONCLUSIONS--The results of this initial epidemiologic study indicate a prevalence of 19% for trichomoniasis in the STD clinic population and 4.6% in the hospitals group. Trichomonas vaginalis was not diagnosed by laboratory methods prior to this study. The InPouch TV test has a selective fungicidal and bactericidal, enriched proteose-peptone medium which provides a sensitivity of 4 organisms per ml and a 1 year shelf life at room temperature. This in vitro culture test demonstrated unique capabilities as a transport and culture medium. Its procedure offers simplicity in application and an excellent visualisation of trichomonads.

Prevalence of genital campylobacteriosis and trichomonosis in crossbred breeding bulls kept on zero-grazed smallholder dairy farms in the Tanga region of Tanzania

“…The inoculation is done into the upper chamber. After that, the pouch is folded down and the contents of the upper chamber are forced into the lower chamber as described by Borchardt et al 2 . Preputial scrapings collected near the fornix, by AI pipette while applying suction with a 20 m syringe were immediately inoculated into the In pouch™ TF test.…”

Cytopathogenic Effect of Trichomonas vaginalis on Human Vaginal Epithelial Cells Cultured In Vitro

“…An estimated 5 million to 10 million Americans and more than 170 million people worldwide are infected annually (16). In underdeveloped countries, the rates of trichomoniasis may vary between 17 and 47% (6,37). In men, the infection is usually asymptomatic, although it may cause irritating urethritis or prostatitis.…”

Development of a Nonhuman Primate Model for Trichomonas vaginalis Infection

“…20 The culture was incubated for 48 hours and then passed to a new InPouch bag. This procedure was repeated 2 more times to ensure the Trichomonas organisms were viable and growing well after freezer storage.…”