A clinical‐grade acellular matrix for esophageal replacement

Arakélian, Lousineh; Caille, Clémentine; Faivre, Lionel; Corté, Laurent; Bruneval, Patrick; Shamdani, Sara; Flageollet, Camille; Albanese, Patricia; Domet, Thomas; Jarraya, Mohamed; Setterblad, Niclas; Kellouche, Sabrina; Larghero, Jérôme; Cattan, Pierre; Vanneaux, Valérie

doi:10.1002/term.2983

Cited by 21 publications

(17 citation statements)

References 38 publications

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“…ECM is composed of around 300 different proteins and carbohydrates, which is mainly composed of collagens, proteoglycans, and glycoproteins (42)(43)(44). So ECM serves as a building support for cell growth in tissue and affect cell migration, lineage commitment, cellular behavior during disease progression, stem cell proliferation and differentiation (24,(45)(46)(47). SDS and triton X-100 can eliminate cellular component and preserve ECM efficiently (48,49).…”

Section: Discussionmentioning

confidence: 99%

Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx

Hou

et al. 2022

Artificial Organs

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Background:Hypopharynx reconstruction after hypopharyngectomy is still a great challenge. Perfusion decellularization is for extracellular matrix (ECM) scaffolding and had been used in organ reconstruction. Our study aimed to prepare an acellular, natural, three-dimensional (3D) biological hypopharynx with vascular pedicle scaffold as the substitute materials to reconstruct hypopharynx.Result:Scanning electron microscope (SEM) and immuno showed that the decellularized hypopharynx with vascular pedicle scaffold retained intact native anatomical ECM structure. Myoblasts were observed on the recellularized scaffolds with bone marrow mesenchyml stem cells (BMSCs) induced by 5-azacytidine implanted in the rabbit greater omentum by immunohistochemical analysis. Conclusion:the decellularized hypopharynx with vascular pedicle scaffold prepared by detergent perfusion in our study has an potential to be an alternative material to pharynx reconstruction.

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Section: Discussionmentioning

confidence: 99%

Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx

Hou

et al. 2022

Artificial Organs

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“…Several biofabrication approaches, including 4-axial printing and electro-spinning with a rotating rodand transplantation of the heterogeneous decellularization organs have been used to fabricate multi-layered tubular constructs [14][15][16][17][18]20,36,37 . However, these technologies require the use of a rotating metal rod of the same size as the diameter of the structure, meaning that the production of constructs with various dimensions is difficult.…”

Section: Discussionmentioning

confidence: 99%

Multi-layered Free-form 3D Cell-printed Tubular Construct with Decellularized Inner and Outer Esophageal Tissue-derived Bioinks

Nam

Jeong

et al. 2020

Sci Rep

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The incidences of various esophageal diseases (e.g., congenital esophageal stenosis, tracheoesophageal fistula, esophageal atresia, esophageal cancer) are increasing, but esophageal tissue is difficult to be recovered because of its weak regenerative capability. There are no commercialized off-the-shelf alternatives to current esophageal reconstruction and regeneration methods. Surgeons usually use ectopic conduit tissues including stomach and intestine, presumably inducing donor site morbidity and severe complications. To date, polymer-based esophageal substitutes have been studied as an alternative. However, the fabrication techniques are nearly limited to creating only cylindrical outer shapes with the help of additional apparatus (e.g., mandrels for electrospinning) and are unable to recapitulate multi-layered characteristic or complex-shaped inner architectures. 3D bioprinting is known as a suitable method to fabricate complex free-form tubular structures with desired pore characteristic. In this study, we developed a extrusion-based 3D printing technique to control the size and the shape of the pore in a single extrusion process, so that the fabricated structure has a higher flexibility than that fabricated in the conventional process. Based on this suggested technique, we developed a bioprinted 3D esophageal structure with multi-layered features and converged with biochemical microenvironmental cues of esophageal tissue by using decellularizedbioinks from mucosal and muscular layers of native esophageal tissues. the two types of esophageal tissue deriveddecellularized extracellular matrix bioinks can mimic the inherent components and composition of original tissues with layer specificity. This structure can be applied to full-thickness circumferential esophageal defects and esophageal regeneration. The esophageal tissue refers to the hollow organ between the oropharynx and the stomach, which allows food to pass to the stomach through peristalsis. Congenital or acquired esophageal disorders such as esophageal cancer, malignancy, and esophageal achalasia usually require reconstruction of the defect site after the surgery and stomach, small and large intestine, and skin tissues are used to repair the esophagus tissues 1-3. Unfortunately, surgical resection and ablation can cause postoperative complications and various surgical morbidities 4-6. Therefore, a tissue engineering-based approach has been proposed as a promising alternative for reconstruction of circumferential esophageal defects 7-9 .

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“…TS-ECM materials can be made into tubular scaffolds, which confer certain potential advantages, such as improved function or performance. Tubular TS-ECM can be used to regenerate blood vessels [171][172][173][174][175][176][177][178], esophagus [179][180][181][182][183][184], bladder [185][186][187], urethra [188,189], ureter [190], urinary conduit [191], bowel [192], and vagina [193]. SIS is one of the best established and most widely applied biomaterials [194].…”

Section: Ts-ecm For Tubular Organ Regenerationmentioning

confidence: 99%

“…In 2018, Luc et al [183] reported a short biologic scaffold comprising decellularized esophageal matrix in a pig model, mimicking native esophagus in in vitro and in vivo characteristics. In 2019, a clinical-grade acellular matrix study reported an esophagus decellularization process, retaining native esophageal ECM structural, biochemical, and biomechanical properties without cytotoxicity, thereby meeting clinical-grade criteria and showing promise for clinical use [184].…”

Section: Ts-ecm For Tubular Organ Regenerationmentioning

confidence: 99%

Decellularized extracellular matrix mediates tissue construction and regeneration

Liu

Pei

et al. 2021

Front. Med.

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Contributing to organ formation and tissue regeneration, extracellular matrix (ECM) constituents provide tissue with three-dimensional (3D) structural integrity and cellular-function regulation. Containing the crucial traits of the cellular microenvironment, ECM substitutes mediate cell—matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo. However, these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures. Cultured cells also produce rich ECM, particularly stromal cells. Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well. Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select, produce, and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration. Overall, the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed. Moreover, current preclinical applications by which ECM components modulate the wound-healing process are reviewed.

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A clinical‐grade acellular matrix for esophageal replacement

Cited by 21 publications

References 38 publications

Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx

Development of a decellularized hypopharynx with vascular pedicle scaffold for use in reconstructing hypopharynx

Multi-layered Free-form 3D Cell-printed Tubular Construct with Decellularized Inner and Outer Esophageal Tissue-derived Bioinks

Decellularized extracellular matrix mediates tissue construction and regeneration

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