A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

Zhang, Na; Liu, Xiong; Qi, Lihua; Chen, Jiali; Qin, Shiyu; Jin, Meiling; Yang, Xiaojing; Liu, Fangni; Guo, Jian; Liu, Jie; Wang, Changjun; Chen, Yong

doi:10.3389/fmicb.2022.1086296

Cited by 6 publications

(2 citation statements)

References 49 publications

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“…Since its first discovery in 2013, K. michiganensis has continued to expand in the clinical setting and has been discovered colonizing patients and causing nosocomial epidemics. 3 , 4 Carbapenems play a critical role in managing severe infections caused by multidrug-resistant Gram-negative bacteria. Currently, the increased incidence of carbapenem-resistant Enterobacteriaceae (CRE), particularly E. coli and K. pneumoniae isolates, is overburdening worldwide healthcare systems.…”

Section: Introductionmentioning

confidence: 99%

“…These β-lactamase genes, which are found on mobile genetic elements and conjugative plasmids, are associated with the widespread dissemination of carbapenem resistance. 7 K. michiganensis producing bla KPC , 4 , 8 , 9 bla VIM , 10 bla NDM 11 , 12 and bla OXA-48 12 have been found and they have been distributed in countries, such as China, 4 Canada, 8 Italy, 9 Japan, 11 Switzerland, 10 and South Africa. 12 However, only sporadic cases have been reported, and the prevalence and transmission patterns of multidrug-resistant K. michiganensis are poorly understood.…”

Section: Introductionmentioning

confidence: 99%

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Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China

Jiang¹,

Li²,

Huang³

et al. 2023

IDR

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Objective Klebsiella michiganensis is an emerging hospital-acquired bacterial pathogen. However, there is a dearth of knowledge on the antimicrobial resistance and transmission of K. michiganensis . Here, we characterized the microbiological and genomic features of a carbapenem-resistant K. michiganensis strain harboring the bla NDM-1 gene in China. Methods K. michiganensis strain 2563 was recovered from the sputum sample of a hospitalized patient with pulmonary infection. Whole-genome sequencing of K. michiganensis strain 2563 was conducted using both the short-read Illumina and long-read MinION platforms to thoroughly characterize the genetic context of bla NDM -carrying plasmid in K. michiganensis 2563. Furthermore, BacWGSTdb server was utilized to perform in silico multilocus sequence typing (MLST), identify antimicrobial resistance genes, and conduct genomic epidemiological analyses of the closely related isolates deposited in the public database. Results K. michiganensis 2563 was resistant to piperacillin, aztreonam, meropenem, imipenem, amoxicillin-clavulanic acid, ampicillin, cefotaxime, cefazolin, ampicillin/sulbactam, cefepime, piperacillin-tazobactam, and ceftazidime. It belonged to sequence type (ST) 43, and the bla NDM-1 gene was found to be located on the plasmid p2563_NDM (54,035 bp). This plasmid showed remarkable similarity to other bla NDM-1 -encoding plasmids found in various Enterobacterium species in the public database. The occurrence of global ST43 K. michiganensis was primarily sporadic, and the closest relative of K. michiganensis 2563 was another ST43 isolate 12,084 recovered from China in 2013, which differed by 171 SNPs. Conclusion Our study reports the genome characteristics of a carbapenem-resistant K. michiganensis strain carrying the bla NDM-1 gene in China, highlighting the need for ongoing surveillance of this pathogen in clinical settings.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China

Jiang¹,

Li²,

Huang³

et al. 2023

IDR

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A nano-biosensing platform based on CuS-BSA for label-free fluorescence detection of Escherichia coli

Zhang,

Li,

Liu

2024

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Molecular epidemiological analysis of blaNDM-5-producing Klebsiella pneumoniae ST2407-K25 causing infection outbreaks in pediatric patients based on whole genome sequencing

Zeng,

Ye,

Hao

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Pediatric patients are vulnerable to the threat of carbapenem-resistant Klebsiella pneumoniae (CRKP) due to their limited immunity and few available antibiotics. Especially when these pathogens exhibit hypervirulent phenotypes, they are often associated with poor clinical outcomes. Methods In this study, we investigated a CRKP outbreak in pediatric patients from 2019 to 2021 in a teaching hospital in China based on whole genome sequencing. We sequenced twenty-nine CRKP isolates isolated from unduplicated pediatric patients to understand their genetic relationships, virulence factors, resistance mechanisms, and transmission trajectories. Conjugation experiments were performed to evaluate the horizontal transfer ability of carbapenem resistance determinants in twenty-nine CRKP isolates. We then characterized these isolates for biofilm formation ability and serum resistance. Genetic relatedness, comparison of plasmids, and chromosomal locus variation of CRKP isolates were analyzed by bioinformatics. Results All the isolates were carbapenemase-producers harbouring bla NDM−5 . Among them, twenty-eight isolates belonged to the ST2407 group, with the consistent capsular serotype K25. The virulence-related factors: ureA , fim , ybtA , irp1/irp2 , and mrkA were prevalent in these isolates. Additionally, most CRKP isolates showed moderately adherent biofilm formation. Although the ST2407 clonal group did not exhibit serum resistance, the heterogeneous level of serum resistance was related to the disruption of oqxR . Conjugation and WGS revealed that the bla NDM−5 carried by the twenty-eight CRKP ST2407 isolates was located on nonconjugative IncX3 plasmids associated with deleting the T4SS-encoding genes. Clonal transmission of CRKP ST2407 in pediatric patients was suggested by the phylogenetic tree. Conclusions Our study provides evidence of the clonal spread of bla NDM−5 -producing K. pneumoniae in pediatric patients and the necessity for the T4SS system for horizontal transfer of the IncX3 plasmid carrying bla NDM−5 . Additionally, the disruption of oqxR may have affected the serum resistance of CRKP. The results of this study emphasize the importance of continuously monitoring for CRKP infection in pediatric patients to prevent recurrent infections. Supplementary Information The online version contains supplementary material available at 10.1186/s12941-024-00747-7.

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A clinical KPC-producing Klebsiella michiganensis strain carrying IncFII/IncFIA (HI1)/IncFIB (K) multiple replicon plasmid

Cited by 6 publications

References 49 publications

Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China

Genomic and Phylogenetic Analysis of a Multidrug-Resistant blaNDM-carrying Klebsiella michiganensis in China

A nano-biosensing platform based on CuS-BSA for label-free fluorescence detection of Escherichia coli

Molecular epidemiological analysis of blaNDM-5-producing Klebsiella pneumoniae ST2407-K25 causing infection outbreaks in pediatric patients based on whole genome sequencing

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