A clinical, molecular and cytogenetic study of 12 cases of human herpesvirus 8 associated primary effusion lymphoma in HIV-infected patients

Boulanger, Emmanuelle; Agbalika, F.; Maarek, Odile; Daniel, Marie‐Thérèse; Grollet, L; Molina, Jean‐Michel; Sigaux, François; Oksenhendler, Éric

doi:10.1038/sj.thj.6200096

Cited by 83 publications

(73 citation statements)

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“…PEL has been rarely observed in HIV-negative patients, and the relatively old age of these patients and/or the presence of visceral failures hampered the use of cytotoxic drugs [11,12]. CHOP has been the most frequently used chemotherapy regimen [2,4,6,7,[13][14][15]. Low-dose m-BACOD [4,6] and COP [16] have been occasionally administered.…”

Section: Discussionmentioning

confidence: 99%

“…For IgH genes, V H -J H rearrangements were amplified by two sets of PCR reactions. For TCR␥ genes, the procedure included three reactions with three different mixtures of primers, as previously described [7]. PCR products were analyzed by polyacrylamide gel electrophoresis.…”

Section: Patients and Methods Patientsmentioning

confidence: 99%

“…PCR detection of KSHV/HHV8 DNA sequences was performed using the previously described quantitative Taqman PCR method [7].…”

Section: Patients and Methods Patientsmentioning

confidence: 99%

“…EBV standard curves have previously been characterized and normalized using the standard curve from human albumin gene and specific sets of primers and 5Ј FAM labeled-probes sequence [9,10]. Amplifications conditions have previously been described [7]. Each specimen was run in duplicate simultaneously with the standards described above.…”

Section: Patients and Methods Patientsmentioning

confidence: 99%

“…In the context of AIDS, most cases are associated with other KSHV/ HHV8-related diseases such as Kaposi's sarcoma (KS) or multicentric Castleman's disease (MCD). The prognosis of PEL is poor, as the median survival in the previously published series does not exceed 3 months [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Combined chemotherapy including high‐dose methotrexate in KSHV/HHV8‐associated primary effusion lymphoma

et al. 2003

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Primary effusion lymphoma (PEL) is a rare KSHV/HHV8-associated high-grade nonHodgkin's lymphoma (NHL) of B-cell origin, characterized by serous effusions in body cavities. Most patients are HIV-infected homosexual men with severe immunosuppression and other KSHV/HHV8-associated diseases such as Kaposi's sarcoma (KS). The prognosis is poor with a median survival of less than 6 months in most cohorts. The achievement of a sustained complete remission is rare. High-dose chemotherapy regimens are warranted to improve complete remission rate and survival. Seven patients with AIDS-associated PEL were treated with a combined chemotherapy including high-dose methotrexate followed by leucovorin rescue. In all cases, KSHV/HHV8 sequences were detected in the effusion samples using quantitative PCR assays. Five patients had a pre-existing KS, associated in three cases with multicentric Castleman's disease (MCD). Upon diagnosis, 6 patients received antiretroviral therapy, which was maintained during chemotherapy in 5 of them. At time of analysis, 3 out of 7 patients were in complete remission 18, 26, and 78 months after PEL diagnosis. Three patients died with a progressive PEL at 22, 67, and 153 days after diagnosis, and 1 patient died 9 months after PEL diagnosis with a MCD-associated plasmablastic NHL. Complete remission was obtained in 3 out of 7 patients treated for AIDS-associated PEL with combined chemotherapy containing high-dose methotrexate. Am.

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