A Clinically Applicable Prediction Model to Improve T Cell Collection in Chimeric Antigen Receptor T Cell Therapy

Get Off on the Right Foot: How to Plan an Efficient Leukocytapheresis to Collect T Cells for CAR T-Cell Manufacturing

“…While the power of the correlation between CD3+ lymphocyte count in peripheral blood and the final target cell yield was high (correlation coefficient r = 0.57–0.86) [ 21 , 25 , 26 ], there is no established minimum peripheral blood lymphocyte count before starting the collection of T cells [ 13 ]. Published series have described successful collections even with cell counts as low as 160–330 lymphocytes/µL [ 13 , 21 , 22 , 25 , 27 , 28 , 29 ]. Although these data demonstrate that with low pre-apheresis cell counts, collection targets can be obtained, it is also true that low pre-apheresis cell counts have been associated with higher collection failure rates [ 21 , 24 , 25 , 28 ].…”

“…Second, the longer the apheresis procedure, the higher the risk for donors to experience adverse events, particularly toxicity due to citrate anticoagulation [ 30 ]. The TBV processed in published series on apheresis for CAR T-cell manufacturing ranges from a median of 4.4–11.6 L [ 12 , 21 , 22 , 24 , 25 , 26 , 27 , 28 , 29 ]. We will show how to adjust the TBV processed by doing simple calculations.…”

“…High variability in cell CE is reported in published studies. In some studies, authors reported mean CE2 of CD3+ cells of 18–68% [ 21 , 23 , 24 , 25 , 26 , 29 ]. Other authors reported CE1 or CE2 of lymphocytes between 43 and 83% [ 22 , 28 ].…”

“…Other authors reported CE1 or CE2 of lymphocytes between 43 and 83% [ 22 , 28 ]. Nevertheless, the vast majority of studies describe an average CE of lymphocytes/CD3+ cells of around 55% [ 21 , 22 , 24 , 25 , 26 , 27 , 29 ].…”

“…Several authors studied different variables that could influence CE in non-mobilized patients. Neither gender nor weight influenced CE [ 12 , 21 , 22 , 29 , 31 ]. Age was negatively correlated with leukocyte CE1 in a published series of adult patients [ 22 , 31 ].…”