A clinico - microbiological study in patients undergoing mechanical ventilation in a tertiary care hospital

Jampala, Baby Latha; Toleti, Sunita; Kolipaka, Sreerama Rao; Myneni, Ramesh Babu

doi:10.18203/2320-6012.ijrms20161964

Cited by 4 publications

(7 citation statements)

References 6 publications

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“…isolates (7.5 %) were isolated during this study. The almost same percentage was isolated in the study of Jampala et al [5] in India. In addition, only one Cryptococcus laurentii was isolated.…”

Section: Discussionsupporting

confidence: 69%

“…The incidence and mortality rate of VAP vary according to the population of patients in ICU, duration of hospital stay, time of onset, causative organisms and prior antimicrobial therapy [1]. Thus, regular monitoring of VAP causative organisms and their antimicrobial susceptibility patterns are essential for quick initiation of the appropriate antimicrobial treatment, thereby reducing the adverse effects on patients' prognosis [5]. Accordingly, this study aimed to determine the microbial profile of clinically suspected VAP cases in ICU patients, under MV, in two tertiary Egyptian hospitals in Cairo, determination of the antimicrobial susceptibility patterns of isolated microorganisms, and investigation of the genetic relatedness and/or diversity among the most frequent isolated microorganism.…”

Section: Introductionmentioning

confidence: 99%

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Microbiological profile of ventilator-associated pneumonia among intensive care unit patients in tertiary Egyptian hospitals

Farag

Tawfick

Abozeed

et al. 2020

J Infect Dev Ctries

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Introduction: Ventilator-associated pneumonia (VAP) is one of the common serious infectious diseases encountered in the intensive care unit (ICU), which highly affects the healthcare cost and patient prognosis. VAP is caused by various antimicrobial-resistant aetiological agents and the clinical manifestations lack sensitivity and specificity, making the prompt treatment is a challenge. This study aimed to investigate the microbial profile of VAP causing microorganisms among ICU patients in Egypt, antimicrobial susceptibility patterns and the genetic diversity among the frequently isolated organisms. Methodology: Throughout the period from August 2016 to August 2017, endotracheal aspirate (ETA) specimens were collected from ICU patients with clinically suspected VAP in two tertiary hospitals in Cairo. ETA specimens were investigated for the microbial content. The antimicrobial susceptibility was determined by the Kirby-Bauer method. ERIC-PCR was performed for genotyping. Results: Fifty microbiologically confirmed VAP cases were identified. The most frequently isolated microorganisms were Klebsiella spp., followed by Pseudomonas aeruginosa, Acinetobacter baumannii. Candida spp. was the most isolated fungi. A single isolate of each Cupriavidus pauculus and Aeromonas salmonicida was isolated. Antimicrobial susceptibility profiles indicated 40% of isolates were multidrug-resistant (MDR). ERIC-PCR revealed no genetic relatedness among K. pneumoniae isolates, the most frequently isolated microorganism. Conclusions: Gram-negative bacteria are the main causative agents of VAP cases, which mostly are MDR. Microorganisms like C. pauculus and A. salmonicida should be taken into consideration as VAP causative agents. There was no common source of infection suggesting likely endogenous sources of K. pneumoniae, the main causative agent of VAP in this study.

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Section: Discussionsupporting

confidence: 69%

Section: Introductionmentioning

confidence: 99%

Microbiological profile of ventilator-associated pneumonia among intensive care unit patients in tertiary Egyptian hospitals

Farag

Tawfick

Abozeed

et al. 2020

J Infect Dev Ctries

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“…In our study, out of the total 100 patients, who were on mechanical ventilation for more than 48 hours 31 were clinically suspected of VAP. The incidence of VAP in our study was 31% which was similar to a study conducted by Jampala et al, Shalini et al and Rakshit et al [5][6][7] The highest percentage of VAP was seen in the age group of 51-60 years (41.9%) followed by 61-70 years (25.8%), 41-50 years (12.9%). Dey et al study showed that patients of age >30 years are more prone to get VAP.…”

Section: Discussionsupporting

confidence: 89%

Untitled

2019

MIJMIC

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Background: VAP is a most common nosocomial infection in the intensive care unit. Knowledge of causative organisms and their antibiotic susceptibility is crucial for diagnosis of VAP in order to initiate the appropriate antibiotic treatment thereby. Aim: To isolate and identify the causative organisms of VAP in ICU patients and also their resistance pattern. Material and Methods: All patients on mechanical ventilation for more than 48 hours were included in the study. The diagnosis of VAP was established using clinical pulmonary infection score (CPIS). Endotracheal aspirate was collected under aseptic precautions after 48 hours of intubation whenever patient was suspected to have developed VAP in ICU and was immediately taken to the laboratory for processing. Results: Out of the 100 cases, VAP was diagnosed in 31 cases. Thus, the incidence of VAP in our study was 31%. Out of total 24 microorganisms isolated, Klebsiella pneumoniae 13 (41.9%) was the most common organism isolated followed by Acinetobacter spp. 6 (19.3%), Pseudomonas aeruginosa 4 (12.9%). Out of the 13 Klebsiella pneumoniae isolates, 11 (84.6%) isolates of Klebsiella pneumoniae were resistant to 3rd generation cephalosporins. Among the 6 isolates of Acinetobacter spp. 4 (33.3%) were resistant to all antibiotics tested. Three Pseudomonas aeruginosa strains were resistant to Piperacillin + tazobactum, Ceftazidime, Cefepime and Ciprofloxacin. Conclusion: This study shows the emergence of MDR Klebsiella pneumoniae, Acinetobacter spp. and Pseudomonas aeruginosa, as potential pathogens causing VAP in our ICU. Combined clinical and microbiological prevention strategies such as rational antibiotic therapy, timely surveillance, strict infection control measures are needed to reduce incidence of VAP.

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“…Similar to the findings of the present study, earlier investigations have noted that Klebsiella species were the most often isolated Gram-negative bacteria from tracheal aspirates and ETT tips, followed by Acinetobacter, Pseudomonas, and Staphylococcus aureus. [24][25] The results of the current investigation showed that while doxycycline was successful, Acinetobacter spp isolates were resistant to the majority of regularly used antibiotics. The outcomes are consistent with several published research [26] , in which Acinetobacter was shown to be very susceptible to polymyxin-b and colistin, intermediately resistant to meropenem and imipenem, and highly resistant to piperacillin tazobactam.…”

Section: Discussionmentioning

confidence: 85%

Microbiological profile and anti-microbial susceptibility pattern of isolates from endo tracheal aspirate and endo tracheal tube tips in ICU patients

Kar

Pattanaik

Nayak

et al. 2022

ijhs

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Background: There has been a surge of sufferers contracting healthcare-acquired infections (HAIs) in recent times. This has been further exacerbated by the SARS-CoV-2 pandemic which, beginning in 2020, has severely affected the majority of the world's nations, and one of the biggest problems caused by this infection was the high number of patients who required critical care. Aim: We sought to identify the microbiological profile and antimicrobial susceptibility pattern of bacteria isolated from tracheal aspirate and endotracheal tube (ETT) tips of patients who had to undergo ICU admittance. Methodology: We isolated a total of 150 microbial samples from 131 patients, of which 97 came from endo tracheal aspirates and the remaining 53 samples were obtained from ETT tips, following which the isolation and identification of bacteria was then done according to requisite microbiological assays. The Kirby-Bauer Technique with Mueller-Hinton agar was used to conduct the antimicrobial susceptibility testing in accordance with Clinical and Laboratory Standards Institute (CLSI) 2016 standards. Results: 53 (35%) of the 150 total specimens were from ETT tips, whereas 97 (65%) came from tracheal aspirates. 101 (67%) of the organisms were gram-negative, and 49 (33%) were gram-positive.

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A clinico - microbiological study in patients undergoing mechanical ventilation in a tertiary care hospital

Cited by 4 publications

References 6 publications

Microbiological profile of ventilator-associated pneumonia among intensive care unit patients in tertiary Egyptian hospitals

Microbiological profile of ventilator-associated pneumonia among intensive care unit patients in tertiary Egyptian hospitals

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Microbiological profile and anti-microbial susceptibility pattern of isolates from endo tracheal aspirate and endo tracheal tube tips in ICU patients

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