A Closterovirus from a Stem-Pitting-Diseased Grapevine

Conti, M.; Milne, Robert G.; Luisoni, E.; Boccardo, G.

doi:10.1094/phyto-70-394

Cited by 78 publications

(36 citation statements)

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“…5 and inset B). GVB.NY particle aggregates, however, were seldom massive as compared to those of GVA [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] or other closteroviruses [12].…”

Section: Cytopathotogymentioning

confidence: 99%

“…The original isolation was made at Geneva (N.Y., U.S.A.), and was subsequently repeated at Bari (Italy) from another Semillon vine supplied some years ago by Dr. A. C. Goheen. The two virus isolates induced the same symptoms in Nicotiana occidental&, had particles of the same size and did not react with an antiserum to grapevine virus A (GVA), another closterovirus with particle length of about 800 nm [2], some isolates of which are mechanically transmissible. As reported in the present paper, a study was carried out on the characterization of this seemingly new closterovirus, for which the name grapevine virus B (GVB) is proposed.…”

Section: Introductionmentioning

confidence: 99%

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Properties of a filamentous virus isolated from grapevines affected by corky bark

Boscia¹,

Savino²,

Minafra³

et al. 1993

Archives of Virology

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A virus with highly flexuous filamentous particles c. 800 nm long, showing distinct transverse striations was isolated with high frequency (60%) by inoculation of Nicotiana occidentalis with sap from grapevine accessions indexing positive for corky bark. The virus, for which the name grapevine virus B (GVB) is proposed, has an ssRNA genome with mol. wt. of c. 2.5 x 10(6) Da (c. 7600 nt) and coat protein subunits with mol. wt. of c 23,000 Da. GVB has a very restricted herbaceous host range and was experimentally transmitted by the mealybug Pseudococcus ficus. The physicochemical and ultrastructural properties of GVB resemble those of closteroviruses. However, it is serologically unrelated to other grapevine closteroviruses including grapevine virus A, with which it shares some biological and physicochemical properties.

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Section: Cytopathotogymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Properties of a filamentous virus isolated from grapevines affected by corky bark

Boscia¹,

Savino²,

Minafra³

et al. 1993

Archives of Virology

View full text Add to dashboard Cite

show abstract

“…Immunoelectron microscopical methods (e.g. Derrick & Brlansky, 1976;Milne & Lesemann, 1978;Roberts et al, 1980;Conti et al, 1980;Nicolaieff & van Regenmortel, 1980;Lesemann & Paul, 1980) have the great advantage of being able to be applied to tissue homogenates and of requiring very low quantities of virus and antiserum. Relationships can be studied in different ways: (i) by the differential trapping of virus particles on electron microscope grids coated with antisera to different viruses; (ii) by endpoint dilution of an antiserum which effectively coats (decorates) the virus particles; (iii) by the observation of a clumping reaction at different serum concentrations.…”

Section: Serology (A) Precipitin Tests Antigenic Analysis Of Isometrmentioning

confidence: 99%

Guidelines for the Identification and Characterization of Plant Viruses

Hamilton

Edwardson

Francki

et al. 1981

Journal of General Virology

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“…Grapevine virus A (GVA) [12] and grapevine virus B (GVB) [-7] are two mechanically transmissible viruses with filamentous particles showing the cross banding and open structure typical of clostero-like viruses [4,10]. GVA was recently shown to be associated with Kober stem grooving [11,16] and GVB with corky bark [7,8], two diseases of grapevine rugose wood complex [9].…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence of the 3? terminal region of the RNA of two filamentous grapevine viruses

Minafra¹,

Saldarelli²,

Grieco³

et al. 1994

Archives of Virology

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The 3' terminal region of grapevine virus A (GVA) and grapevine virus B (GVB), encompassing 1883 and 2136 nucleotides, respectively, was sequenced by the deoxynucleotide chain termination method. Three putative open reading frames (ORF) were identified in both genomic viral RNAs, denoted 1 to 3 in the 5' to 3' direction. ORF 1 encoded a polypeptide with estimated M(r) of 31 kDa (GVA) and 36.5 kDa (GVB), possessing the G/D motif of the "30 K superfamily" movement proteins, and showing good alignments with putative movement proteins of trichoviruses and capilloviruses. ORF 2 was identified as the coat protein (CP) cistron, coding for polypeptides with an estimated M(r) of 21.5 kDa (GVA) and 21.6 kDa (GVB). These CPs showed substantial sequence homology with one another and with CPs of tricho- and capilloviruses, but not of closteroviruses. ORF 3 potentially coded for two small polypeptides with estimated M(r) of 10 kDa (GVA) and 14 kDa (GVB). The ORF 3 product of GVB (14 K), but not that of GVA, shared some homology with the 3' terminal polypeptides of different plant viruses that exhibit the "zinc finger domain" of proteins with nucleic acid-binding properties. GVA and GVB have many properties in common with trichoviruses but possess an extra open reading frame (ORF 3). Whether this finding may have a bearing on the classification of these viruses is unclear. However, until the taxonomic significance of this difference in genome structure is established, it seems plausible to include GVA and GVB as tentative species in the Trichovirus genus.

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A Closterovirus from a Stem-Pitting-Diseased Grapevine

Cited by 78 publications

References 0 publications

Properties of a filamentous virus isolated from grapevines affected by corky bark

Properties of a filamentous virus isolated from grapevines affected by corky bark

Guidelines for the Identification and Characterization of Plant Viruses

Nucleotide sequence of the 3? terminal region of the RNA of two filamentous grapevine viruses

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