A co-fermentation strategy with wood hydrolysate and crude glycerol to enhance the lipid accumulation in Rhodosporidium toruloides-1588

Saini, Rahul; Osorio‐González, Carlos S.; Hegde, Krishnamoorthy; Brar, Satinder Kaur; Vezina, Pierre

doi:10.1016/j.biortech.2022.127821

Cited by 14 publications

(3 citation statements)

References 37 publications

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“…Recent studies of R. toruloides are concentrated on metabolic engineering, especially lipid production from biomass hydrolysates [ 67 – 69 ], with less attention to aspects of non-lipid metabolism [ 13 , 15 , 40 , 49 , 70 , 71 ]. More of these studies are needed to identify rate-limiting steps, enzyme redundancy, and cofactor preference of major catabolic pathways to improve productivity, yields, titers, and efficiency of bioproduction.…”

Section: Discussionmentioning

confidence: 99%

Non-canonical d-xylose and l-arabinose metabolism via d-arabitol in the oleaginous yeast Rhodosporidium toruloides

2023

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R. toruloides is an oleaginous yeast, with diverse metabolic capacities and high tolerance for inhibitory compounds abundant in plant biomass hydrolysates. While R. toruloides grows on several pentose sugars and alcohols, further engineering of the native pathway is required for efficient conversion of biomass-derived sugars to higher value bioproducts. A previous high-throughput study inferred that R. toruloides possesses a non-canonical l-arabinose and d-xylose metabolism proceeding through d-arabitol and d-ribulose. In this study, we present a combination of genetic and metabolite data that refine and extend that model. Chiral separations definitively illustrate that d-arabitol is the enantiomer that accumulates under pentose metabolism. Deletion of putative d-arabitol-2-dehydrogenase (RTO4_9990) results in > 75% conversion of d-xylose to d-arabitol, and is growth-complemented on pentoses by heterologous xylulose kinase expression. Deletion of putative d-ribulose kinase (RTO4_14368) arrests all growth on any pentose tested. Analysis of several pentose dehydrogenase mutants elucidates a complex pathway with multiple enzymes mediating multiple different reactions in differing combinations, from which we also inferred a putative l-ribulose utilization pathway. Our results suggest that we have identified enzymes responsible for the majority of pathway flux, with additional unknown enzymes providing accessory activity at multiple steps. Further biochemical characterization of the enzymes described here will enable a more complete and quantitative understanding of R. toruloides pentose metabolism. These findings add to a growing understanding of the diversity and complexity of microbial pentose metabolism.

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Section: Discussionmentioning

confidence: 99%

Non-canonical d-xylose and l-arabinose metabolism via d-arabitol in the oleaginous yeast Rhodosporidium toruloides

2023

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“…A total lipid titer of 10.6 g/L with lipid yields of 0.25 g lipids/g consumed carbon was observed. Saini et al combined a woody hemicellulosic hydrolysate with crude glycerol in a 60:40 ratio, which resulted in the consumption of 90% of the sugars and glycerol from the media and reporting a maximum intracellular lipid content of 56.3% (w/w) [ 95 ]. In that work, biomass and lipid volumetric productivity values were 0.28 g/L h and 0.15 g/L h, respectively.…”

Section: Lignocellulosic Biomass and Industrial Wastes As Raw Materialsmentioning

confidence: 99%

Challenges and prospects of yeast-based microbial oil production within a biorefinery concept

Gallego-García,

Susmozas,

Negro

et al. 2023

Microb Cell Fact

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Biodiesel, unlike to its fossil-based homologue (diesel), is renewable. Its use contributes to greater sustainability in the energy sector, mainly by reducing greenhouse gas emissions. Current biodiesel production relies on plant- and animal-related feedstocks, resulting in high final costs to the prices of those raw materials. In addition, the production of those materials competes for arable land and has provoked a heated debate involving their use food vs. fuel. As an alternative, single-cell oils (SCOs) obtained from oleaginous microorganisms are attractive sources as a biofuel precursor due to their high lipid content, and composition similar to vegetable oils and animal fats. To make SCOs competitive from an economic point of view, the use of readily available low-cost substrates becomes essential. This work reviews the most recent advances in microbial oil production from non-synthetic sugar-rich media, particularly sugars from lignocellulosic wastes, highlighting the main challenges and prospects for deploying this technology fully in the framework of a Biorefinery concept.

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“…The quest to establish fourth-generation biofuels has fostered basic and applied research as an alternative to renewable energy sources, mainly when associated with agricultural waste [158,159]. Special attention has yet to be given to lignocellulosic sources and crude glycerol, a by-product of biodiesel production, which may generate an associated production chain and become technologically and economically viable shortly [160]. In addition to its use as a biofuel, there is the prospect of using it as an automotive lubricant additive [137] and wax ester [161].…”

Section: Microbial Lipid-based Productsmentioning

confidence: 99%

Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects

Silva

Martins

Moreira

et al. 2023

Foods

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The use of lignocellulosic biomass as a raw material for the production of lipids has gained increasing attention, especially in recent years when the use of food in the production of biofuels has become a current technology. Thus, the competition for raw materials for both uses has brought the need to create technological alternatives to reduce this competition that could generate a reduction in the volume of food offered and a consequent commercial increase in the value of food. Furthermore, the use of microbial oils has been studied in many industrial branches, from the generation of renewable energy to the obtainment of several value-added products in the pharmaceutical and food industries. Thus, this review provides an overview of the feasibility and challenges observed in the production of microbial lipids through the use of lignocellulosic biomass in a biorefinery. Topics covered include biorefining technology, the microbial oil market, oily microorganisms, mechanisms involved in lipid-producing microbial metabolism, strain development, processes, lignocellulosic lipids, technical drawbacks, and lipid recovery.

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A co-fermentation strategy with wood hydrolysate and crude glycerol to enhance the lipid accumulation in Rhodosporidium toruloides-1588

Cited by 14 publications

References 37 publications

Non-canonical d-xylose and l-arabinose metabolism via d-arabitol in the oleaginous yeast Rhodosporidium toruloides

Non-canonical d-xylose and l-arabinose metabolism via d-arabitol in the oleaginous yeast Rhodosporidium toruloides

Challenges and prospects of yeast-based microbial oil production within a biorefinery concept

Microbial Lipid Based Biorefinery Concepts: A Review of Status and Prospects

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