A Coagglutination Assay with Monoclonal Antibodies for Rapid Laboratory Identification of Mycoplasma synoviae

Morsy, Mohamad A.; Panangala, Victor S.; Gresham, Martha M.; Toivio-Kinnucan, M.

doi:10.2307/1591730

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…The coagglutination test described above proved to be useful for detecting P. haemolytica type-specific antigens, as previously shown for other bacterium species and genera (2,5,20,22,26,27,29), even in the absence of viable P. haemolytica.…”

Section: Discussionsupporting

confidence: 61%

Coagglutination test for serotyping Pasteurella haemolytica

1996

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A coagglutination test was described for simple, fast, and reliable detection of Pasteurella haemolytica type-specific antigens in lung lesions even in the absence of viable P. haemolytica. The coagglutinating reagents were prepared by coating protein A-producing Staphylococcus aureus cells with hyperimmune sera raised against P. haemolytica type strains. Bacterial suspensions, saline extracts, and boiled saline extracts of the bacteria were used as antigens. Homologous reactions with all types of antigens were precise. Some crossreactions were similar to those obtained by the indirect hemagglutination test, and some additional one-way cross-reactions were identified. The coagglutination test was used for serotyping 65 P. haemolytica field strains and for the detection of P. haemolytica type-specific antigens in the lung specimens of 62 calves and 78 sheep. Ninety-four percent of the field strains could be serotyped by the coagglutination test. P. haemolytica typespecific antigens were detected in the lung specimens of 3 calves and 5 sheep that had succumbed to naturally occurring P. haemolytica pneumonia and in the lungs of 20 calves experimentally infected with P. haemolytica A1. The coagglutination test detected type-specific antigens in 36% of the lung specimens of slaughtered field sheep but not in the lungs of slaughtered field cattle with small chronic lung lesions. No reaction occurred in the case of nonpneumonic calves and sheep or when pneumonic lesions were caused by other bacteria. No P. haemolytica strains could be isolated from lung samples that were coagglutination test negative. This test is recommended as an additional method for fast and reliable serotyping of P. haemolytica. Pasteurella haemolytica is a well-known pathogen of ruminants worldwide. P. haemolytica has two biotypes (31): biotype A, which causes pneumonia in cattle and sheep (13, 17, 34), mastitis in ewes (17, 33), and occasionally arthritis and meningitis in sheep (17); and biotype T, which is responsible for ovine systemic pasteurellosis of feeding lambs (9). Some authors have suggested the creation of a new species, Pasteurella trehalosi, for strains of the latter biotype (32). Several methods have been used for the serologic typing of P. haemolytica. The tube agglutination test, used for the examination of somatic antigens, did not prove to be practical because of numerous cross-reactions (3, 4). Biberstein et al. (3, 4) introduced the indirect hemagglutination (IHA) test for serotyping P. haemolytica and were able to identify 12 serotypes. Modifications to the IHA test made the test easier and faster (15). IHA became the most widespread method for the examination of P. haemolytica serotypes. The double diffusion test and countercurrent immunoelectrophoresis can also be used for serotyping P. haemolytica (10). For the rapid serotyping of P. haemolytica strains, Frank and Wessman (14) introduced a slide agglutination test, which gave results similar to those obtained with the IHA test. However, some P. haemolytica strains could n...

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Section: Discussionsupporting

confidence: 61%

Coagglutination test for serotyping Pasteurella haemolytica

1996

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Coagglutination test for rapid detection of infectious bursal disease virus antigen

Shakya¹,

Joshi²

1997

Trop Anim Health Prod

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Rapid diagnosis of fowl pox with coagglutination assay

Joshi¹,

Shakya²

1997

Trop Anim Health Prod

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The coagglutination test was standardised for detection of fowl pox antigen in infected scabs and chorioallontoic membrane of chicken embryos. The Staphylococcus aureus Cowan I strain, containing large amounts of Protein A in their cell wall, coated with fowl pox antibodies was found specific and sensitive for detection of fowl pox antigen. The test is easy to perform and rapid as the positive results can be read within 15 seconds.

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A Coagglutination Assay with Monoclonal Antibodies for Rapid Laboratory Identification of Mycoplasma synoviae

Cited by 7 publications

References 22 publications

Coagglutination test for serotyping Pasteurella haemolytica

Coagglutination test for serotyping Pasteurella haemolytica

Coagglutination test for rapid detection of infectious bursal disease virus antigen

Rapid diagnosis of fowl pox with coagglutination assay

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