A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis

Harinath, B. C.; Kumar, Satish; Roy, Santa Saha; Hirudkar, Suvarna; Upadhye, Vijay; Shende, Niraj

doi:10.1016/j.diagmicrobio.2005.10.021

Cited by 16 publications

(7 citation statements)

References 22 publications

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“…A cocktail of affinity purified antibodies to antigens ES-31, ES-43 and EST-6 (ES-41+ES-38) showed a sensitivity of 91% for detection of antigen and 97% for IC-Ag with specificity of 95% and 99% respectively by sensitive penicilinase immune assay. Similar observations were made for detection of antibody and antigen in patients of extra pulmonary tuberculosis [30,31]. In a prospective study, the in house developed SEVA TB ELISA using cocktail of ES-31 and EST-6 antigens showed 100% correlation (42 cases) with AFB positivity and ATT treatment [32].…”

Section: Diagnostically Important Excretory Secretory (Es) Proteinssupporting

confidence: 48%

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Waghmare¹,

Wankhade²,

Jena³

et al. 2016

Mycobact Dis

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TB immunodiagnostics based on antibody and antigen detection for early detection and monitoring tubercular infections at low cost and flexible to adapt to field laboratories are a boon to developing countries. In this context we have explored in-house developed penicillinase based ELISA assays for the detection of antigen, antibody as well as immune-complexed antigen using various excretory secretory antigenic proteins and specific antibodies. Excretory secretory protein antigens such as ES-31, ES-41, ES-43, ES-6, ES-20, ES-100 and EST-6 were studied extensively and found to be useful in various pulmonary and extra pulmonary cases of tuberculosis infection. ES-31 has shown its diagnostic potential in chronic PTB cases, ES-43 in relapse cases and ES-41 in bone and joint TB. Elevated level of ES-20 antigen was observed in patients with weak immune response in TB lymphadenitis. Detection of ES-100 antigen and antibody by penicilinase ELISA was observed to be useful in detection of TB meningitis. ES-6 antigen was shown to be useful in detection of latent infection. These proteins with antigenic properties are utilized for production of specific antibodies against them and observed to be useful in immune diagnostics for detection of specific circulating and immune complexed antigens. Further user friendly peroxidase immunoassay has been standardised and is being routinely used for suspected TB cases attending 1000 bedded Kasturba Hospital attached to medical institute on physician's request.

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Section: Diagnostically Important Excretory Secretory (Es) Proteinssupporting

confidence: 48%

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Waghmare¹,

Wankhade²,

Jena³

et al. 2016

Mycobact Dis

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“…Acid-fast staining test on sputum smear usually has very low sensitivity (about 30–40%) [ 8 ]. As the gold standard for TB diagnosis, sputum smear and culture test is time-consuming that needs 4–8 weeks to obtain result, which is not suitable for rapid TB diagnosis and treatment [ 9 , 10 ]. PCR-based nucleic acid amplification assays significantly improved rapid diagnosis of TB, but amplification of endogenous inhibition factor of Mtb and the lack of reliable quality control have resulted in high false positive and false negative rates [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Liu¹,

Qie²,

et al. 2016

BioMed Research International

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Rapid and accurate diagnosis of pulmonary tuberculosis (PTB) is an unresolved problem worldwide, especially for sputum smear− (S−) cases. In this study, five antigen genes including Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 were cloned from Mycobacterium tuberculosis (Mtb) RD1 and overexpressed to generate antigen fragments. These antigens and their combinations were investigated for PTB serodiagnosis. 298 serum samples were collected from active PTB patients, including 117 sputum smear+ (S+) and sputum culture+ (C+) cases, 101 S−/C+ cases, and 80 S−/C− cases. The serum IgG levels of the five antigens were measured by ELISA. Based on IgG levels, the sensitivity/specificity of Rv3871, Rv3874, Rv3875, Rv3876, and Rv3879 for PTB detection was 81.21%/74.74%, 63.09%/94.78%, 32.21%/87.37%, 62.42%/85.26%, and 83.56%/83.16%, respectively. Furthermore, the optimal result for PTB diagnosis was achieved by combining antigens Rv3871, Rv3876, and Rv3879. In addition, the IgG levels of Rv3871, Rv3876, and Rv3879 were found to be higher in S−/C+ PTB patients than in other PTB populations. More importantly, combination of the three antigens demonstrated superior diagnostic performance for both S−/C+ and S−/C− PTB. In conclusion, the combination of Rv3871, Rv3876, and Rv3879 induced higher IgG response in sputum S−/C+ PTB patients and represents a promising biomarker combination for diagnosing of PTB.

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“…A study performed in India compared a sandwich ELISA test for ES-31, ES-43, and EST-6 with the monospecific anti-ES-31 antibody detection in 68 smear-positive cases of tuberculosis and showed a sensitivity of 91-97% for the three antigens compared to 79-91% for the monospecific test. 46 A number of antigens which can be detected when present at a concentration of 3-20ng/mL include mycobacterial sonicates, tuberculin purified protein derivative (PPD) and antigens 5, A60, P32 and LAM, detected through sandwich or inhibition ELISA, latex agglutination or reverse passive hemagglutination (RPHA) tests. 11,47 The reported sensitivity of these tests is quite low, roughly between 40-50%, with slightly higher specificities, of 80-95%.…”

Section: Antigen Detectionmentioning

confidence: 99%

Recent advances in the diagnosis of Mycobacterium tuberculosis

Anochie¹,

Onyeneke²,

Ogu³

et al. 2012

GERMS

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Molecular technologies offer the greatest potential for laboratories in resource-rich countries because they have the highest sensitivity and specificity.Continued use of new technologies will be crucial in elucidating the true epidemiology and pathogenesis of a disease, including the less well studied diseases.Continued development of affordable, sensitive, and specific diagnostic tools will be required for use in resource-poor settings, where the incidence of disease is highest.

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A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis

Cited by 16 publications

References 22 publications

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Identification of Novel RD1 Antigens and Their Combinations for Diagnosis of Sputum Smear−/Culture+ TB Patients

Recent advances in the diagnosis of Mycobacterium tuberculosis

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