People perceive out-groups, minorities, and novel groups more negatively than in-groups, majorities, and familiar groups. Previous research has argued that such intergroup biases may be caused by the order in which people typically encounter social groups. Groups that are relatively novel to perceivers (e.g., out-groups, minorities) are primarily associated with distinct attributes that differentiate them from familiar groups. Because distinct attributes are typically negative, attitudes toward novel groups are negatively biased. Five experiments ( N = 2,615 adults) confirmed the generalizability of the novel groups’ disadvantage to different aspects of attitude formation (i.e., evaluations, memory, stereotyping), to cases with more than two groups, and to cases in which groups were majority/minority or in-groups/out-groups. Our findings revealed a remarkably robust influence of learning order in the formation of group attitudes, and they imply that people often perceive novel groups more negatively than they actually are.