A Collagen‐Derived Oligopeptide from Salmo salar Collagen Hydrolysates Restrains Atherogenesis in ApoE–/– Mice via Targeting P2Y12 Receptor

Yang, Yijie; Liu, Hui; Cui, Liyuan; Liu, Yibo; Fu, Lulu; Лі, Бо

doi:10.1002/mnfr.202200166

Cited by 4 publications

(1 citation statement)

References 37 publications

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“…Numerous studies have already demonstrated that P2Y 12 receptors possess the capacity to amplify VSMC proliferation and migration [ 22 , 23 , 47 , 48 ], consequently exerting a noteworthy role in atherogenesis. We hypothesize that the augmented expression of P2Y 12 receptors within VSMCs in stenotic AVF venous tissues could similarly propel VSMC proliferation and migration, thereby fostering neointimal growth.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Liu,

Gao,

Tang

et al. 2023

Renal Failure

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Objective This study investigated the role of the P2Y 12 receptor in autogenous arteriovenous fistula (AVF) failure resulting from stenosis. Methods Stenotic venous tissues and blood samples were obtained from patients with end-stage renal disease (ESRD) together with AVF stenosis, while venous tissues and blood samples were collected from patients with ESRD undergoing initial AVF surgery as controls. Immunohistochemistry and/or immunofluorescence techniques were utilized to assess the expression of P2Y 12 , transforming growth factor-β1 (TGF-β1), monocyte chemotactic protein 1 (MCP-1), and CD68 in the venous tissues. The expression levels of P2Y 12 , TGFβ1, and MCP-1 were quantified using quantitative reverse transcription–polymerase chain reaction and western blot analyses. Double and triple immunofluorescence staining was performed to precisely localize the cellular localization of P2Y 12 expression. Results Expression levels of P2Y 12 , TGFβ1, MCP-1, and CD68 were significantly higher in stenotic AVF venous tissues than in the control group tissues. Double and triple immunofluorescence staining of stenotic AVF venous tissues indicated that P2Y 12 was predominantly expressed in α-SMA-positive vascular smooth muscle cells (VSMCs) and, to a lesser extent, in CD68-positive macrophages, with limited expression in CD31-positive endothelial cells. Moreover, a subset of macrophage-like VSMCs expressing P2Y 12 were observed in both stenotic AVF venous tissues and control venous tissues. Additionally, a higher number of P2Y 12 + /TGF-β1 + double-positive cells were identified in stenotic AVF venous tissues than in the control group tissues. Conclusion Increased expression of P2Y 12 in stenotic AVF venous tissues of patients with ESRD suggests its potential involvement in the pathogenesis of venous stenosis within AVFs.

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Section: Discussionmentioning

confidence: 99%

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Liu,

Gao,

Tang

et al. 2023

Renal Failure

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Structural Insights on Hyp-Gly-Containing Peptides as Antiplatelet Compounds through Topomer CoMFA and CoMSIA Analysis

Yang

Tian

et al. 2023

Foods

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Increasing evidence has shown collagen hydrolysate involves a variety of bioactivities. In our previous study, multiple antiplatelet peptides containing Hyp/Pro-Gly were identified in collagen hydrolysates from Salmo salar and silver carp skin and exhibited anti-thrombosis activity without bleeding risks in vivo. However, the relationship between structure and activity remains unknown. We performed 3D-QSAR studies on 23 Hyp/Pro-Gly-containing peptides in which 13 peptides were reported before. CoMFA, Topomer CoMFA and CoMSIA analyses were used to generate the QSAR models. Topomer CoMFA analysis showed a q2 value of 0.710, an r2 value of 0.826, an r2pred value of 0.930, and the results showed that Hyp instead of Pro was more important for improving the antiplatelet activity. CoMSIA analysis showed a q2 value of 0.461, an r2 value of 0.999, and an r2pred value of 0.999. Compared with the electrostatic field and hydrogen bond donor field, the steric field, hydrophobic field and hydrogen bond receptor field have great influence on the activity of antiplatelet peptides. The predicted peptide EOGE exhibited antiplatelet activity induced by ADP, and inhibited thrombus formation (300 μmol/kg bw) without bleeding risks. Combined results of these studies indicate that OG-containing peptides had a potential to be developed into an effective specific medical food in the prevention of thrombotic diseases.

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Effects of Marine-Derived Components on Cardiovascular Disease Risk Factors and Gut Microbiota Diversity

Lamminpää,

Amedei,

Parolini

2024

Marine Drugs

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Cardiovascular diseases (CVDs), which comprise coronary heart disease, hypertension, and stroke, collectively represent the number one cause of death globally. Atherosclerosis is the dominant cause of CVDs, and its risk factors are elevated levels of low-density lipoprotein cholesterol and triglycerides, hypertension, cigarette smoking, obesity, and diabetes mellitus. In addition, diverse evidence highlights the role played by inflammation and clonal haematopoiesis, eventually leading to immunity involvement. The human microbiota project and subsequent studies using next-generation sequencing technology have indicated that thousands of different microbial species are present in the human gut. Disturbances in the gut microbiota (GM) composition, i.e., gut dysbiosis, have been associated with diseases ranging from localised gastrointestinal disorders to metabolic and cardiovascular illnesses. Of note, experimental studies suggested that GM, host immune cells, and marine-derived ingredients work together to ensure intestinal wall integrity. This review discusses current evidence concerning the links among GM, marine-derived ingredients, and human inflammatory disease. In detail, we summarise the impact of fish-derived proteins/peptides and algae components on CVD risk factors and gut microbiome. Furthermore, we describe the interplay among these dietary components, probiotics/prebiotics, and CVDs.

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A Collagen‐Derived Oligopeptide from Salmo salar Collagen Hydrolysates Restrains Atherogenesis in ApoE^–/– Mice via Targeting P₂Y₁₂ Receptor

Cited by 4 publications

References 37 publications

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Structural Insights on Hyp-Gly-Containing Peptides as Antiplatelet Compounds through Topomer CoMFA and CoMSIA Analysis

Effects of Marine-Derived Components on Cardiovascular Disease Risk Factors and Gut Microbiota Diversity

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A Collagen‐Derived Oligopeptide from Salmo salar Collagen Hydrolysates Restrains Atherogenesis in ApoE–/– Mice via Targeting P2Y12 Receptor

Cited by 4 publications

References 37 publications

Increased expression of the P2Y 12 receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Increased expression of the P2Y 12 receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Structural Insights on Hyp-Gly-Containing Peptides as Antiplatelet Compounds through Topomer CoMFA and CoMSIA Analysis

Effects of Marine-Derived Components on Cardiovascular Disease Risk Factors and Gut Microbiota Diversity

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Product

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A Collagen‐Derived Oligopeptide from Salmo salar Collagen Hydrolysates Restrains Atherogenesis in ApoE^–/– Mice via Targeting P₂Y₁₂ Receptor

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis

Increased expression of the P2Y ₁₂ receptor is involved in the failure of autogenous arteriovenous fistula caused by stenosis