A colloidal gold immunochromatography test strip based on a monoclonal antibody for the rapid detection of triadimefon and triadimenol in foods

Abstract

In this study, we produced a specific monoclonal antibody (mAb) against triadimefon (TDF) and triadimenol (TDN). The half-maximal inhibitory concentration for TDF and TDN was 5.25 and 9.98 ng/ mL, respectively, with a linear detection range (IC 20-IC 80) of 1.09-25.35 and 2.29-43.61 ng/mL, respectively. Subsequently, we developed a colloidal gold immunochromatography test strip for the analysis of TDF and TDN in tomato. The results obtained from the colloidal gold immunochromatography test strip were in accordance with those obtained from the indirect competitive enzyme-linked immunosorbent assay. The colloidal gold immunochromatography test strip is efficient and suitable for onsite detection of TDF and TDN.

Sensitive Lateral Flow Immunoassay for the Residues of Imidocarb in Milk and Beef Samples

“…Finally, 50 μL/well of the 0.01 M H 2 SO 4 solution was used to stop the color reaction, and the absorbance was measured at 450 nm using a microplate reader. 25,26 Several parameters can affect the performance and sensitivity of the ic-ELISA, and this study optimized two of the important terms of the working solution, namely, pH and ionic strength. 27−29 A series of standard dilution buffers at different pH values (pH 4.0, 5.5, 7.4, 8.8, and 9.6) were used to dilute the imidocarb standards.…”

“…After one more washing step, 100 μL/well of the TMB/peroxide-based substrate solution was added by incubation at 37 °C for 15 min. Finally, 50 μL/well of the 0.01 M H 2 SO 4 solution was used to stop the color reaction, and the absorbance was measured at 450 nm using a microplate reader. , …”

An Immunochromatographic Assay for the Rapid and Qualitative Detection of Mercury in Rice

“…After vortexing, the sample was centrifuged at 6500 r/min for 15 min, and the supernatant was removed. The pH of the supernatant was adjusted to 7.4 with 0.75 M sodium hydroxide [ 28 ]. Five instances of dilution with HBS were used to obtain the final sample extract.…”

Colloidal Au sphere and nanoflower-based immunochromatographic strips for sensitive detection of zearalenone in cereals

“…Therefore, the gold nanoparticles produced by shorter and cheaper procedures are still one of the most widely used materials today in various fields. 40–42…”

“…Therefore, the gold nanoparticles produced by shorter and cheaper procedures are still one of the most widely used materials today in various elds. [40][41][42] Colloidal Au spheres (AuSPs) are a certain type of gold particles produced aer various reducing agents acting on chloroauric acid, and they exhibit a stable colloidal state in solvents. 43,44 AuSPs are widely used due to their easy synthesis, low cost, and good chemical stability.…”