A Colloidal Gold Nanoparticle-Based Immunochromatographic Test Strip for Rapid and Convenient Detection of Staphylococcus Aureus

Abstract

An immunochromatographic test strip using gold nanoparticles-staphylococcus aureus monoclonal antibody conjugates was developed for the rapid and convenient detection of staphylococcus aureus based on a double-antibody sandwich format. The detection limit and the detection rate of this test strip is 10(3) CFU /mL and 98.7%, respectively. It could be used for the rapid detection of staphylococcus aureus in food and the results can be visually identified by the naked eye within 10 min. Compared with conventional bacterial detection methods, this developed immunochromatographic assay based test strip has several advantages including simple, fast, low-cost, favorable sensitivity and specificity, exhibiting a great potential for application in food safety control systems and clinical diagnosis.

A Graphene Oxide–Based Sensing Platform for the Determination of Methicillin-Resistant Staphylococcus aureus Based on Strand-Displacement Polymerization Recycling and Synchronous Fluorescent Signal Amplification

“…Importantly, the lower detection limit reached 3 × 10 2 CFU/ mL, which is superior to those of surface plasmon resonance (SPR), colorimetric, and fluorescence biosensors. [28][29][30] The specificity of this designed platform was also estimated by surveying the fluorescence of target bacteria and six other bacteria: S. aureus, S. epidermidis, S. saprophyticus, S. typhimurium, E. coli, and S. dysenteriae. Figure 5 shows a comparison among the fluorescent intensities in response to MRSA and other nontarget bacteria.…”

Microbiological food safety in Malaysia from the academician’s perspective

“…Jung et al (2005) developed an assay on to detect E. coli O157. Besides that, Niu et al (2014) detected S. aureus while Xu et al (2013) detected C. jejuni. Detection of toxins such as brevetoxins and staphylococcal enterotoxin B was also possible with lateral flow immunoassay (Zhou et al, 2009;Rong-Hwa et al, 2010).…”

Chromatographic Immunoassays: Strategies and Recent Developments in The Analysis of Drugs and Biological Agents

“…Other chromatographic sandwich immunoassays have been described for IL-5, anti-HCV antibodies, potato virus X, cardiac troponin and myoglobin [81][82][83][84]. Sandwich immunoassays have also been applied to the determination of allergens, bacteria and toxins in food [85][86][87][88][89].…”

“…For example, the use of nanomaterials, such as gold or silver nanoparticles, and quantum dots should continue to lead to new or enhanced detection methods and formats for these methods [19,[85][86][87][88][93][94][95][96][97][98][99][100]109]. The utilization of alternative supports, such as affinity monoliths, is also expected to continue [110][111][112][113][114][115][116].…”

“…The utilization of alternative supports, such as affinity monoliths, is also expected to continue [110][111][112][113][114][115][116]. In addition, further research is anticipated in the development of miniaturized chromatographic immunoassays that can be employed in microfluidic devices and portable or disposable devices [19,[26][27][28]30,[82][83][84][85][86][87][88]100,113,116]. These develop ments, in turn, should increase the range of applications for which chromatographic immunoassays can be used in areas that include clinical chemistry, pharmaceutical testing, drug development and biomedical research [7,8].…”