A Coloration Biochip for Optical Virus Detection Based on Printed Single Nanoparticle Array

Abstract

Direct and ultrasensitive detection of nanoscale objects is of great importance for materials science and biomedical application. Here, a printed single nanoparticle array‐based coloration biochip is developed for direct visualizing nanoscale objects with the microscopy image. Arising from the scattering cross section induced Mie scattering enhancement, the color change of nanoparticles with/without viruses can be observed with a conventional optical microscope. The scattering light from single nanoparticle has the size‐dependent characteristics of wavelength and intensity variation, allowing for optically detecting nanoscale virus. By comparing optical images before and after attaching viruses, quantitative detection can be achieved with the virus concentrations from 1.0 × 102 to 1.0 × 106 PFU mL–1 and a detection limit of 1.0 × 102 PFU mL–1. The antibody‐modified array can specifically recognize influenza viruses in 15 min. The result provides a promising approach to detect diverse biological samples, including protein, exosomes, and platelet microparticle.

Multioxide combinatorial libraries: fusing synthetic approaches and additive technologies for highly orthogonal electronic noses

“…Printing technologies such as nano-printing and 3D printing are costeffective, robust, and highly scalable. [58][59][60][61][62] These technologies enable the fabrication of fully printed arrays, for example, photonic crystal microarrays for the detection of urinary KIM-1, as demonstrated by Liu et al 63 The resolution of these technologies has reached such low limits that it is possible to print nanoparticle arrays used for virus detection, as shown by Wang et al 64 Dai et al reviewed printing technologies utilized specifically for gas sensors marginalizing such methods as inkjet printing, aerosol jet printing, and fused deposition modelling, among others. 65 Su and Song also discussed various printing strategies and their applications.…”

Multilayer Langmuir Film of Monodisperse Au Nanoclusters: Unusual Growth via Bilayers

“…The need for miniaturization requires developing novel approaches on how to build even smaller, yet functional devices. One of these approaches is by utilizing nanoparticles and incorporating them into devices. − Either individual nanoparticles can be utilized or nanoparticles assembled into a thin layer. − …”

“…One of these approaches is by utilizing nanoparticles and incorporating them into devices. 1−5 Either individual nanoparticles can be utilized 6 or nanoparticles assembled into a thin layer. 7−10 In order to prepare organized films of nanomaterials, selfassembly methods are commonly used.…”