1945
DOI: 10.1016/s0021-9258(17)41484-0
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A Colorimetric Determination of Blood Acetoin

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Cited by 640 publications
(38 citation statements)
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“…The mixture was incubated for an additional 25 min at 37°C to allow for the acid hydrolysis of the acetolactate to acetoin. The acetoin formed was then measured using the Voges-Proskauer test (Eddy, 1961;Westerfeld, 1945) A 0.2-mL aliquot of the aforementioned mixture was transferred to a tube with 0.8 mL of 0.45N NaOH. A 1-mL aliquot of 1:1 volume ratio ␣-naphthol (5% ␣-naphthol in 2.5N NaOH) to 0.5% creatine was added and the resulting mixture maintained at room temperature for 30 min with shaking.…”
Section: B Subtilis Als Assaymentioning
confidence: 99%
“…The mixture was incubated for an additional 25 min at 37°C to allow for the acid hydrolysis of the acetolactate to acetoin. The acetoin formed was then measured using the Voges-Proskauer test (Eddy, 1961;Westerfeld, 1945) A 0.2-mL aliquot of the aforementioned mixture was transferred to a tube with 0.8 mL of 0.45N NaOH. A 1-mL aliquot of 1:1 volume ratio ␣-naphthol (5% ␣-naphthol in 2.5N NaOH) to 0.5% creatine was added and the resulting mixture maintained at room temperature for 30 min with shaking.…”
Section: B Subtilis Als Assaymentioning
confidence: 99%
“…Acetolactate synthase activity was tested as described previously [ 25 ]. Acetolactate produced after one hour was assayed at a single endpoint by conversion to acetoin, which was detected using the reaction method described by Westerfeldt [ 26 ] and quantified through the use of a standard curve after subtraction of the acetoin produced without substrate. The acetoin content was then normalized based on the protein content.…”
Section: Methodsmentioning
confidence: 99%
“…Acetolactate synthase activity was examined as described previously ( Muhitch, 1988 ). The acetolactate produced after 1 h was assayed at a single end point by conversion to acetoin, which was detected by the reaction of Westerfeldt ( Westerfeldt, 1945 ) and quantified through the use of a standard curve after subtraction of the acetoin produced without substrate. Acetoin content was then normalized by the protein content.…”
Section: Methodsmentioning
confidence: 99%