A colorimetric Loop-mediated isothermal amplification (LAMP) assay based on HRP-mimicking molecular beacon for the rapid detection of Vibrio parahaemolyticus

Lee, Jeong Eun; Mun, Hyoyoung; Kim, Se-Ri; Kim, Min-Gon; Chang, Ji-Yoon; Shim, Won‐Bo

doi:10.1016/j.bios.2019.111968

Cited by 69 publications

(27 citation statements)

References 32 publications

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“…These techniques can detect any nucleic acids and are not specific for COVID-19 LAMP products. Additionally, electrophoresis is time-consuming and tedious, while for colored dyes and indicators, the judgment of color change in tubes by eye is potentially subjective ( Lee et al, 2020 ). Therefore, there is an ongoing need to develop new LAMP-based assays that are capable of simultaneously detecting multiple SARS-CoV-2 targets to provide a rapid and more objective result, as well as to facilitate simpler diagnostic tests.…”

Section: Introductionmentioning

confidence: 99%

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Zhu

Wang

Han

et al. 2020

Biosensors and Bioelectronics

359

256

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The ongoing global pandemic (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a huge public health issue. Hence, we devised a multiplex reverse transcription loop-mediated isothermal amplification (mRT-LAMP) coupled with a nanoparticle-based lateral flow biosensor (LFB) assay (mRT-LAMP-LFB) for diagnosing COVID-19. Using two LAMP primer sets, the ORF1ab (opening reading frame 1a/b) and N (nucleoprotein) genes of SARS-CoV-2 were simultaneously amplified in a single-tube reaction, and detected with the diagnosis results easily interpreted by LFB. In presence of FITC (fluorescein)-/digoxin- and biotin-labeled primers, mRT-LAMP produced numerous FITC-/digoxin- and biotin-attached duplex amplicons, which were determined by LFB through immunoreactions (FITC/digoxin on the duplex and anti-FITC/digoxin on the test line of LFB) and biotin/treptavidin interaction (biotin on the duplex and strptavidin on the polymerase nanoparticle). The accumulation of nanoparticles leaded a characteristic crimson band, enabling multiplex analysis of ORF1ab and N gene without instrumentation. The limit of detection (LoD) of COVID-19 mRT-LAMP-LFB was 12 copies (for each detection target) per reaction, and no cross-reactivity was generated from non-SARS-CoV-2 templates. The analytical sensitivity of SARS-CoV-2 was 100% (33/33 oropharynx swab samples collected from COVID-19 patients), and the assay's specificity was also 100% (96/96 oropharynx swab samples collected from non-COVID-19 patients). The total diagnostic test can be completed within 1 h from sample collection to result interpretation. In sum, the COVID-19 mRT-LAMP-LFB assay is a promising tool for diagnosing SARS-CoV-2 infections in frontline public health field and clinical laboratories, especially from resource-poor regions.

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Section: Introductionmentioning

confidence: 99%

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Zhu

Wang

Han

et al. 2020

Biosensors and Bioelectronics

359

256

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“…In this sense, Horseradish peroxidase mimicking DNAzyme (HRPzyme) forms a G‐quadruplex structure that mimics peroxidase activity after binding to hemin, and catalyzes the oxidation of 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) or 3,3′,5,5′ tetramethylbenzidine (TMB) in the presence of H 2 O 2 , which produces a colored product. This approach was used in combination with LAMP for the detection of V. parahaemolyticus ; in this study, positive samples displayed a faint blue color, whereas negative reactions displayed very intense color (Lee et al., 2020). Similar approaches have been reported for other pathogens (Liu et al., 2018; Zhu et al., 2017).…”

Section: Naked‐eye Detectionmentioning

confidence: 96%

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Garrido‐Maestu

Prado

2022

Comp Rev Food Sci Food Safe

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Nucleic acid amplification‐based techniques have gained acceptance by the scientific, and general, community as reference methodologies for many different applications. Since the development of the gold standard of these techniques, polymerase chain reaction (PCR), back in the 1980s many improvements have been made, and alternative techniques emerged reporting improvements over PCR. Among these, isothermal amplification approaches resulted of particular interest as could overcome the need of specialized equipment to accurately control temperature changes, but it was after year 2000 that these techniques have flourished in a huge number of novel alternatives with many different degrees of complexities and requirements. An added value is their possibility to be combined with many different naked‐eye detection strategies, simplifying the resources needed, allowing to reduce cost, and serving as the basis for novel developments of lab‐on‐chip systems, and miniaturized devices, for point‐of‐care testing. In this review, we will go over different types of naked‐eye detection strategies, combined with isothermal amplification. This will provide the readers up‐to‐date information for them to select the most appropriate strategies depending on the particular needs and resources for their experimental setup.

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“…It has been utilized for the alternative detection of certain diseases because of its low cost. At present, LAMP has been applied to the identification and quantification of pathogenic bacteria with significant advantages in sensitivity, specificity, and rapidity [95,132]. Since LAMP requires four primers specifically designed for six different regions of the target, any incomplete matching of the primers will theoretically lead to the phenomenon of non-reaction and nonspecific amplification.…”

Section: Loop-mediated Isothermal Amplification (Lamp)mentioning

confidence: 99%

Molecular Methods for Pathogenic Bacteria Detection and Recent Advances in Wastewater Analysis

Zhang

et al. 2021

Water

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With increasing concerns about public health and the development of molecular techniques, new detection tools and the combination of existing approaches have increased the abilities of pathogenic bacteria monitoring by exploring new biomarkers, increasing the sensitivity and accuracy of detection, quantification, and analyzing various genes such as functional genes and antimicrobial resistance genes (ARG). Molecular methods are gradually emerging as the most popular detection approach for pathogens, in addition to the conventional culture-based plate enumeration methods. The analysis of pathogens in wastewater and the back-estimation of infections in the community, also known as wastewater-based epidemiology (WBE), is an emerging methodology and has a great potential to supplement current surveillance systems for the monitoring of infectious diseases and the early warning of outbreaks. However, as a complex matrix, wastewater largely challenges the analytical performance of molecular methods. This review synthesized the literature of typical pathogenic bacteria in wastewater, types of biomarkers, molecular methods for bacterial analysis, and their recent advances in wastewater analysis. The advantages and limitation of these molecular methods were evaluated, and their prospects in WBE were discussed to provide insight for future development.

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A colorimetric Loop-mediated isothermal amplification (LAMP) assay based on HRP-mimicking molecular beacon for the rapid detection of Vibrio parahaemolyticus

Cited by 69 publications

References 32 publications

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Multiplex reverse transcription loop-mediated isothermal amplification combined with nanoparticle-based lateral flow biosensor for the diagnosis of COVID-19

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Molecular Methods for Pathogenic Bacteria Detection and Recent Advances in Wastewater Analysis

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