A Colorimetric Method for the Estimation of Uracil and Cytosine

Soodak, Morris; Pircio, Anthony W.; Cerecedo, Leopold R.

doi:10.1016/s0021-9258(18)56594-7

Cited by 16 publications

(2 citation statements)

References 9 publications

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“…The brominated pyrimidine is unstable and the double bond would be regenerated in the conditions of the fluorimetric 1972 assay. This can be avoided by treating the dried sample with a solution of alkali in the form of Na2CO3, which serves to degrade the brominated pyrimidine ring (Soodak et al, 1949). A portion (0.4ml) of 0.2M-Na2CO3 is added.…”

Section: Labilization Ofthe Pyrimidine-glycosidic Bondmentioning

confidence: 99%

“…have been described (Massart & Hoste, 1947;Soodak et al, 1949;Yanagisawa & Isono, 1966;Trim & Parker, 1970). The method that we have used is not dissimilar to these, but it is necessary to show that it enables pyrimidine deoxyribose derivatives to be determined accurately, and that it does not interfere with the determination of the purine derivatives.…”

Section: Fluorimetric Assaymentioning

confidence: 99%

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A procedure for the isolation and determination of deoxyribose derivatives

Pestell

Woodland

1972

Biochemical Journal

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A method of determining deoxyribose derivatives in biological material is described. It has high sensitivity, and is particularly useful in that it can be applied to a large range of tissues for which the other available assays are unsuitable. This is because the method is applicable to complex mixtures of nucleotides in which such substances as ribonucleotides are present in very large excess over deoxyribonucleotides, and it is not necessary to equilibrate the nucleotide-precursor pool with radioactive phosphate. The method has mainly been developed with the object of determining deoxyribonucleoside triphosphates, but it can be used to assay ribonucleoside triphosphates, as well as the mono- and diphosphates of both types of nucleoside. The procedure used involves three basic techniques: (1) periodate oxidation and methylamine-induced cleavage of the sugar ring to destroy 2'- and 3'-unsubstituted ribonucleosides; (2) column chromatography to separate the deoxyribonucleotides from each other and from other substances, such as the products of the periodate oxidation; (3) fluorimetric determination of deoxyribose after labilization of the pyrimidine-glycosidic bond by bromination of the heterocyclic ring. Each of these three procedures can be used independently, in conjunction with other analytical procedures.

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Section: Labilization Ofthe Pyrimidine-glycosidic Bondmentioning

confidence: 99%

Section: Fluorimetric Assaymentioning

confidence: 99%

A procedure for the isolation and determination of deoxyribose derivatives

Pestell

Woodland

1972

Biochemical Journal

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Procedure for Assay and Stability Determination of Idoxuridine

Simpson¹,

Zappala²

1964

Journal of Pharmaceutical Sciences

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A Screening Method for Dihydropyrimidine Dehydrogenase Deficiency with Colorimetric Detection of Urinary Uracil

Okajima

Yamamoto

Suchi

et al. 1989

Advances in Experimental Medicine and Biology

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Dlhydropyrimidine dehydrogenase deficiency has a neurological involvement as a common symptom among reported cases. No major sympTom except that exists for DHPDH deficiency. On the other hand. relationship ~elween neuroiogical lnvolvement and metabolic disorder is stIli obsc~re. ror toe purpose 01 looking for more patients with DHPDH deficiency. a screening method for DHPDB deficiency is introduced.Urinary uracil was determined colorimetrically. ThiS method is not so complicated and less time ronsuming as previous method such as liquid chromatography. With this method. it is possible to detect about 1 I1IllIol·l (12 mg/dl) of uracil. which is sensitive enough for the screening for DHPDH deficiency. Interfering substance in urine were negligible. Addition of albumin to normal urine dose not affect the result but proteinuria results in false posi tive.The urine from 83 epileptic children were screened with this method. but no patients were found. 119 K. Mikanagi et al. (eds.), Purine and Pyrimidine Metabolism in Man VI

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A Colorimetric Method for the Estimation of Uracil and Cytosine

Cited by 16 publications

References 9 publications

A procedure for the isolation and determination of deoxyribose derivatives

A procedure for the isolation and determination of deoxyribose derivatives

Procedure for Assay and Stability Determination of Idoxuridine

A Screening Method for Dihydropyrimidine Dehydrogenase Deficiency with Colorimetric Detection of Urinary Uracil

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