A colorimetric method for the determination of choline and acetylcholine in small amounts

Beattie, Florence

doi:10.1042/bj0301554

Cited by 91 publications

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“…Iodine numbers were determined by Yasuda's (1931) modification of the Hanus method, and carbon and hydrogen analyses were made according to the standard microcombustion method of Pregl. Choline was estimated as the reineckate according to the technique described by Beattie (1936). Amino nitrogen determinations were carried out by the Van Slyke volumetric procedure.…”

Section: Fractionation Of Lipidmentioning

confidence: 99%

Studies on the gonococcus

1944

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Progress in biochemical research on the gonococcus has not kept pace with that on other pathogenic microorganisms of similar importance. Reasons for this are twofold: gonococcal infection comparable to that in man cannot be reproduced experimentally in animals and the organism is difficult to grow in sufficient quantities to permit chemical study. For the latter reason, few of the cellular constituents of the gonococcus have been characterized. Warden (1915) has reported the presence of lipid of high complexity. Boor and Miller (1934) described the immunologic properties of a "nucleoprotein" fraction of the gonococcus as well as those of a group-specific carbohydrate .Casper (1937), on the other hand, claimed the isolation of type-specific carbohydrates from agar cultures of recently isolated strains. No quantitative chemical studies, however, have been carried out. Microanalytical methods are now available which permit the accurate determination of a number of cellular constituents.Employing such methods, analyses have been made on mass cultures of 6 strains of Neisseria gonorrhoeae grown for 3, 6, and 10 days in broth media. Observations at these different intervals made it possible to determine the change in the amounts of cellular constituents throughout the life of the cell. Analytical data are presented on two nucleoprotein fractions which have been isolated and described. In addition, the lipid has been separated into a number of crystalline and non-crystalline fractions, several of which have been analyzed and identified. MATERIALS AND METHODS CulturesSix strains of Neisseria gonorrhoeae were studied. Two of them,2 1 and 2, were isolated from the male urethra. Strain 1 had been under cultivation for 32 years, while strain 2 was isolated approximately 2 weeks prior to mass cultivation. Strains 3 and 4, isolated from the cervix and from a male urethra had been under artificial cultivation for 1 and 5 months, respectively. Strains 5 and 6 were recovered from the cervix 14 months and 3 months, respectively, prior to use. Each strain was grown in Douglas's broth (Hartley, 1922) containing 0.05 per 1 This study was made possible by a grant from the John and Mary R. Markle Foundation. 2 Mass cultures were supplied through the courtesy of the research laboratories of Parke, Davis and Company, Detroit, Michigan. The cultures were grown for 6 days in a veal infusion, hormone broth, harvested in a Sharples centrifuge and without further treatment dried to constant weight in vacuo over calcium chloride. 129 on August 5, 2020 by guest

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Section: Fractionation Of Lipidmentioning

confidence: 99%

Studies on the gonococcus

1944

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“…Der Gesamt-N wurde nach Kjeldahl in der Mikroausführung von Pregl, Amino-N nach van Slyke, Cholin-N nach Beattie 15 ), Colamin-N nach B l ix 2 ), Phosphor nach Embden 16 Fettsäuren. An gesättigten Säuren 10,13 g (1), an ungesättigten Säuren 41,45 g (2).…”

Section: Experimenteller Teilunclassified

Serin als stickstoffhaltiger Bestandteil der Glycerinphosphatide des Menschengehirns

Schuwirth¹

1942

Hoppe-Seyler´s Zeitschrift für physiologische Chemie

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Im Verlauf der vorliegenden Arbeit, aus welcher Teilergebnisse bereits früher in Form einer vorläufigen Mitteilung veröffentlicht wurden 1 ), konnte Serin als neue Stickstoffkomponente in den Glycerinphosphatiden des Menschengehirns auf Grund folgender Beobachtungen nachgewiesen werden: 1. Der Colaminstickstoffgehalt der hydrolytischen Spaltprodukte der Glycerinphosphatide, bestimmt nach einer neuen, von Blix 2 ) angegebenen Methode, deren Brauchbarkeit vorher nachgeprüft wurde, ist stets bedeutend geringer als der nach van Slyke bestimmbare Aminostickstoffgehalt; er beträgt rund 50°/ 0 desselben. 2. Serin wurde in Form der /9-Naphthalinsulfosäure Verbindung nach Fischer und Bergeil 3 ) aus den wasserlöslichen Spaltprodukten der Glycerinphosphätide isoliert. 3. Es ließ sich freies Serin nach Spaltung der Glycerinphosphatide mit Ba(OH) 3 nach vorausgehender Extraktion d §s Hydrolysates mit n-Butylalkohol isolieren.Die Nachprüfung der von Blix 2 ) angegebenen Colaminbestimmungsmethode, welche auf der Flüchtigkeit des Colamins bei 75-80° im Wasserstrahlvakuum bei Abwesenheit von Wasser beruht, ergab mit reinen Colaminlösungen gute Kesultate (vgl. Tab. 2). Weder die Anwesenheit größerer Cholinmengen noch eine Vorbehandlung mit NaOH in Siedehitze sind von Einfluß auf das Ergebnis. Die Anwendbarkeit der Methode zur Colaminbestimmung in den wasserlöslichen Spaltprodukten der Glycerinphosphatide ließ sich durch einen Versuch, bei welchem 86,4°/ 0 der aus dem Titrationsergebnis berechneten Colaminmenge aus dem Destillat durch Kondensation mit Phthalsäureanhydrid isoliert wurde, dartun. Bei der beabsichtigten Anwendung der Bestimmungsmethode auf Phosphatidhydrolysate störte die längst Brought to you by | Purdue University Libra

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“…Evans and Partridge (10) used ammonium reineckate for the characterization of two sola~laceous alkaloids (hyoscine and hyoscyamine) obtained by partition chromatography from Atropa belladon~ia and Datura stramoniz~nz and Fried and Wintersteiner (12) were able to separate two high molecular weight water-soluble bases (streptomycin ancl streptothricin) from submerged-culture filtrates of the respective Actinomyces in the form of cr);stalline reineckates. In general, these derivatives are quite insoluble in water and because they have a relatively high molecular weight a number of workers have utilized their formation for the quantitative cletermination of small amounts of many organic bases (2,3,4,5,9,10,19,20,24,26). As a result of these investigations amnloniu~n reineckate has become one of the most versatile reagents for the identification and estimation of ainines.…”

Section: Introductionmentioning

confidence: 99%

The Characterization of Narcotics as Reineckates

Levi¹,

Farmilo²

1952

Can. J. Chem.

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A m m o n i~~m reineclcate was found to react with methorphinan hydrobromide, codeine phosphate, and the hydrochlorides of methadone, phenadosonc, pjpidone, cocai~~e, pethidine, ketobernidone, alphaprodine, morphine, dianlorphrne, and metopon to yield characteristic crystals. The molecular composition, decomposition range, optical rotation, solubility in water and ethanol, and spectral transmittance of these crystalline compounds are reported with the pKrj values of the narcotics in water and in 50y0 acetone.

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A colorimetric method for the determination of choline and acetylcholine in small amounts

Cited by 91 publications

References 0 publications

Studies on the gonococcus

Studies on the gonococcus

Serin als stickstoffhaltiger Bestandteil der Glycerinphosphatide des Menschengehirns

The Characterization of Narcotics as Reineckates

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