A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Taylor‐Robinson, David; Purcell, Robert H.; Wong, D.; Chanock, Robert M.

doi:10.1017/s0022172400040377

Cited by 293 publications

(121 citation statements)

References 17 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…The growth-inhibition test may be less sensitive than the complement-fixation test, although the converse has been shown to hold for other Mycoplasma spp. by Taylor-Robinson, Purcell, Wong & Chanock (1966). We think that growth-inhibiting antibody probably appears in smaller amounts only in the more prolonged or severe infections, and disappears more quickly than complementfixing antibody.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

The distribution of complement-fixing antibody and growth-inhibiting antibody to Mycoplasma hominis

Jones

Sequeira²

1966

Epidemiol. Infect.

View full text Add to dashboard Cite

Sera from 3163 patients were examined for growth-inhibiting and complement-fixing antibody to Mycoplasma hominis. The results were analysed in respect of the age and sex of the patients. Antibodies were found to be uncommon in young people but increased in frequency with age; they were present in old men but were less common in women over sixty-five. Patients attending V.D. clinics and prison inmates had a much higher incidence of antibody than hospital and ante-natal patients. Complement-fixing antibody was approximately three times as common as growth-inhibiting antibody in the sample examined; it was also more common in women than men but the overall incidence of growth-inhibiting antibody was the same in each sex. It was concluded that possibly growth-inhibiting antibody is produced in more severe infections and that it disappears from the serum more quickly than complement-fixing antibody.

show abstract

Section: Discussionmentioning

confidence: 89%

“…The test was performed in the manner of Taylor-Robinson, Purcell, Wong & Chanock (1966) but modified by replacing the glucose in the medium with arginine . The metabolism of arginine by the mycoplasms produces an increase in the pH of the medium resulting in a colour change.…”

Section: Growth-inhibition Testmentioning

confidence: 99%

The distribution of complement-fixing antibody and growth-inhibiting antibody to Mycoplasma hominis

Jones

Sequeira²

1966

Epidemiol. Infect.

View full text Add to dashboard Cite

show abstract

“…The growth inhibition (GI) and the metabolic inhibition (MI) tests were carried out essentially as described elsewhere (7,53). The plate immunofluorescence antibody (FA) test was performed both with the direct and indirect techniques (1 2,46).…”

Section: Methodsmentioning

confidence: 99%

Spiroplasma citri gen. and sp. n.: A Mycoplasma-Like Organism Associated with "Stubborn" Disease of Citrus

Saglio

L'Hospital

Laflèche

et al. 1973

International Journal of Systematic Bacteriology

322

153

View full text Add to dashboard Cite

“…Antisera to strains 128T and 547 were prepared as described by Morton and Roberts (18) and Hill (11). The following three seroiogicai methods were used: growth inhibition tests with antiserum-impregnated sterile paper disks (4), metabolism inhibition tests in microtiter plates (13,21,22,27), and immunoperoxidase tests with colonies grown on agar (20). All of the tests were carried out in duplicate.…”

Section: Methodsmentioning

confidence: 99%

Mycoplasma oxoniensis, a New Species Isolated from Chinese Hamster Conjunctivas

Hill

1991

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

Mycoplasmas which were distinct from Mycoplasma cricetuli were isolated from the conjunctivas of Chinese hamsters. Two clones, which were derived from a single colony and were obtained on separate occasions, were examined in detail for morphology, growth, and biochemical characteristics. These clones were indistinguishable from each other and had the following properties: guanine-plus-cytosine content of 29 mol% , requirement for sterol, facultatively anaerobic, and positive for glucose metabolism. They were serologically distinct from M . cricetuli and all 94 other previously described Mycoplasma and Acholepksma spp. One of them, strain 128 (= NCTC 11712), is designated the type strain of a new species, Mycoplasma oxoniensis.Previously, only one named Mycoplasma species has been isolated from Chinese hamsters. Mycoplasma cricetuli was isolated from the conjunctivas of Chinese hamsters in several animal colonies in the United Kingdom that were mostly derived from one original group (12, 14). M . cricetuli was also recovered from Chinese hamsters in The Netherlands (19a).Recently, other mycoplasma strains that appeared to be serologically and otherwise distinct from M . cricetuli were isolated from Chinese hamsters (unpublished data). In this paper I describe tests that were carried out to characterize two of these isolates in order to examine their relationships to each other and to M . cricetuli and also to provide the criteria necessary for determining whether they represent a new species (26). Other strains isolated had similar properties. MATERIALS AND METHODSMycoplasma strains. Mycoplasmas were isolated from the conjunctivas of Chinese hamsters from an animal colony on two separate occasions. A culture of each isolate was cloned to produce a pure culture by using a method involving initial filtration of a broth culture through a 300-nm-pore-size membrane filter, culturing of the filtrate on solid medium, transfer of a single resulting colony to another agar plate, and inoculation of the subsequent growth into broth. This whole procedure was repeated an additional four times; thus, the organisms were filter cloned five times (26). One of the clones was designated strain 12gT (T = type strain), and the other clone was designated strain 547.Mycoplasma species. The following Acholeplasma spp. type strains were used:Medium and growth conditions. The culture medium used has been described by and by Hill (11). The purified agar used in the growth medium was Lab M agar (Lab M, London, England). Depending on their biochemical activities, the mycoplasmas were grown in liquid medium containing 1% (wt/vol) glucose (pH 7.8) or in liquid medium containing 1% (wt/vol) arginine (pH 7.3). The species grown in medium containing arginine were not inhibited by 1% arginine. Agar cultures were incubated at 35 to 37°C either in a humid chamber or under anaerobic conditions in a GasPak system. Liquid cultures were stored at -70°C in ampules.Growth requirements and characteristics. subcultured onto solid medium and incubated for 1...

show abstract

A colour test for the measurement of antibody to certain mycoplasma species based upon the inhibition of acid production

Cited by 293 publications

References 17 publications

The distribution of complement-fixing antibody and growth-inhibiting antibody to Mycoplasma hominis

The distribution of complement-fixing antibody and growth-inhibiting antibody to Mycoplasma hominis

Spiroplasma citri gen. and sp. n.: A Mycoplasma-Like Organism Associated with "Stubborn" Disease of Citrus

Mycoplasma oxoniensis, a New Species Isolated from Chinese Hamster Conjunctivas

Contact Info

Product

Resources

About