A combination of methylprednisolone and quercetin is effective for the treatment of cardiac contusion following blunt chest trauma in rats

Abstract

Cardiac contusion is a potentially fatal complication of blunt chest trauma. The effects of a combination of quercetin and methylprednisolone against trauma-induced cardiac contusion were studied. Thirty-five female Sprague-Dawley rats were divided into five groups (n=7) as follows: sham, cardiac contusion with no therapy, treated with methylprednisolone (30 mg/kg on the first day, and 3 mg/kg on the following days), treated with quercetin (50 mg·kg−1·day−1), and treated with a combination of methylprednisolone and quercetin. Serum troponin I (Tn-I) and tumor necrosis factor-alpha (TNF-α) levels and cardiac histopathological findings were evaluated. Tn-I and TNF-α levels were elevated after contusion (P=0.001 and P=0.001). Seven days later, Tn-I and TNF-α levels decreased in the rats treated with methylprednisolone, quercetin, and the combination of methylprednisolone and quercetin compared to the rats without therapy, but a statistical significance was found only with the combination therapy (P=0.001 and P=0.011, respectively). Histopathological degeneration and necrosis scores were statistically lower in the methylprednisolone and quercetin combination group compared to the group treated only with methylprednisolone (P=0.017 and P=0.007, respectively). However, only degeneration scores were lower in the combination therapy group compared to the group treated only with quercetin (P=0.017). Inducible nitric oxide synthase positivity scores were decreased in all treatment groups compared to the untreated groups (P=0.097, P=0.026, and P=0.004, respectively). We conclude that a combination of quercetin and methylprednisolone can be used for the specific treatment of cardiac contusion.

Effects of thymoquinone against cisplatin-induced cardiac injury in rats

“…Slides stained with hematoxylineeosin were evaluated in terms of congestion, edema and pycnotic nuclei parameters in randomly chosen six different areas in each slide under x40 objective magnification. The slides were scored according to the method described by Demir et al 14 were considered as statistically significant.…”

Assessment of the Therapeutic Effect of Resveratrol in Heart Failure Following Blunt Chest Trauma in Rats and the Potential Role of Endokan as a Biomarker of Inflammation

“…Myocyte damage, hemorrhages, and edema may be observed at the microscopic level. [11] Blunt chest trauma also results in the onset of inflammatory processes and release of mediators, leading to life-threatening hemodynamic changes and arrhythmias. Heart injury after blunt chest trauma is associated with an increase in mortality rates.…”

“…Heart injury after blunt chest trauma is associated with an increase in mortality rates. [10,11] The present study examined the anti-inflammatory efficacy of resveratrol in cardiac damage induced by blunt chest trauma.…”

“…[5] Further research suggested that resveratrol regulated metabolism, glucose, and lipid levels and that it had positive effects on Çağlar Torun et al Assessing the therapeutic effect of resveratrol in heart failure following blunt chest trauma and the potential role of endocan life-saving. [10,11] However, blunt chest trauma can be difficult to detect due to inadequate diagnostic methods and lack of a gold standard. The mortality rate after blunt chest trauma increases in the presence of heart failure.…”

Cardiac Contusions

“…Currently, there are attempts to apply some of the principles used to manage other forms of myocardial injury (ischemia, cardiac surgery) to CC. Data from an animal study by Demir et al is encouraging demonstrating that treatment with methylprednisolone and quercetin may be beneficial in CC [43]. Rat models with CC had a significant reduction in Tn-I and TNF-alpha levels at 7 days, when treated with a combination of methylprednisolone and quercetin, compared to those without treatment.…”