A Combination of Rosa Canina Extracts and Gold Complex Favors Apoptosis of Caco-2 Cells by Increasing Oxidative Stress and Mitochondrial Dysfunction

Mármol, Inés; Jiménez-Moreno, Nerea; Ancín‐Azpilicueta, Carmen; Osada, Jesús; Cerrada, Elena; Rodríguez-Yoldi, María Jesús

doi:10.3390/antiox9010017

Cited by 12 publications

(5 citation statements)

References 33 publications

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“…During the last few years, our research group has investigated the chemopreventive properties of extracts obtained from different plant matrices such as rosehips, fenugreek, pine bark, and artichoke waste on human colon cancer [ 3 , 25 , 26 , 27 ]. Though there have only been few publications on this subject, the anticarcinogenic potential of grape stem extracts has also been studied in different cell lines [ 13 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Grape Stem Extracts with Potential Anticancer and Antioxidant Properties

et al. 2021

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The application of plant extracts for therapeutic purposes has been used in traditional medicine because plants contain bioactive compounds with beneficial properties for health. Currently, the use of these compounds that are rich in polyphenols for the treatment and prevention of diseases such as cancer, diabetes, and cardiovascular diseases, many of them related to oxidative stress, is gaining certain relevance. Polyphenols have been shown to have antimutagenic, antioxidant, and anti-inflammatory properties. Therefore, the objective of the present work was to study the potential effect of grape stem extracts (GSE), rich in phenolic compounds, in the treatment of cancer, as well as their role in the prevention of this disease associated with its antioxidant power. For that purpose, three cancer lines (Caco-2, MCF-7, and MDA-MB-231) were used, and the results showed that grape stem extracts were capable of showing an antiproliferative effect in these cells through apoptosis cell death associated with a modification of the mitochondrial potential and reactive oxygen species (ROS) levels. Additionally, grape stem extracts showed an antioxidant effect on differentiated intestinal cells that could protect the intestine from diseases related to oxidative stress. Therefore, grape extracts contain bioactive principles with important biological properties and could be used as bio-functional food ingredients to prevent diseases or even to improve certain aspects of human health.

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Section: Introductionmentioning

confidence: 99%

Grape Stem Extracts with Potential Anticancer and Antioxidant Properties

et al. 2021

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“…88 Recently, some authors assessed the extract of rosehip against different cancer cell lines, including colon, lung, prostate, cervix, liver, brain, and breast, suggesting its potential role in chemotherapy. 25,89 After treating these cancer cell lines with whole rose hip extract or purified fractions of its most important components, all studies have reported a noticeable decline in cell viability. These antioxidant properties may be responsible for their antiproliferative effect.…”

Section: Discussionmentioning

confidence: 99%

Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment

Bonilla-Vidal,

Świtalska,

Espina

et al. 2023

IJN

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Cancer is one of the major causes of death worldwide affecting more than 19 million people. Traditional cancer therapies have many adverse effects and often result in unsatisfactory outcomes. Natural flavones, such as apigenin (APG), have demonstrated excellent antitumoral properties. However, they have a low aqueous solubility. To overcome this drawback, APG can be encapsulated in nanostructured lipid carriers (NLC). Therefore, we developed dual NLC encapsulating APG (APG-NLC) with a lipid matrix containing rosehip oil, which is known for its anti-inflammatory and antioxidant properties. Methods: Optimisation, physicochemical characterisation, biopharmaceutical behaviour, and therapeutic efficacy of this novel nanostructured system were assessed. Results: APG-NLC were optimized obtaining an average particle size below 200 nm, a surface charge of −20 mV, and an encapsulation efficiency over 99%. The APG-NLC released APG in a sustained manner, and the results showed that the formulation was stable for more than 10 months. In vitro studies showed that APG-NLC possess significant antiangiogenic activity in ovo and selective antiproliferative activity in several cancer cell lines without exhibiting toxicity in healthy cells. Conclusion: APG-NLC containing rosehip oil were optimised. They exhibit suitable physicochemical parameters, storage stability for more than 10 months, and prolonged APG release. Moreover, APG-NLC were internalised inside tumour cells, showing the capacity to cause cytotoxicity in cancer cells without damaging healthy cells.

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“…However, tucum extract demonstrated a potent pro-oxidant effect in Caco-2 cells by increasing mitochondrial ROS production that was not observed in intestinal fibroblasts, thus indicating a cell type-specific action. The pro-oxidant effect of catechins is related to apoptosis induction in tumor cells, such as HL-60, HT-29 and RAW 264.7 due to interference with cellular signaling pathways or by increasing H 2 O 2 and hydroxyl radical production under experimental conditions (36)(37)(38)(39). ROS production is increased when Rosa canina is combined with a drug to treat colorectal cancer, but when Caco-2 cells treated with hydrogen peroxide are exposed to only Rosa canina's extract, ROS production decreases, showing an antioxidant effect (40,41).…”

Section: Discussionmentioning

confidence: 99%

Pro-Oxidant and Cytotoxic Effects of Tucum-Do-Cerrado (Bactris setosa Mart.) Extracts in Colorectal Adenocarcinoma Caco-2 Cells

Silva

Fagundes

Faber

et al. 2022

Nutrition and Cancer

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Colorectal cancer is one of the most common types of cancer. Bioactive natural compounds can act in cancer chemoprevention as tumor growth inhibitors. Tucum-do-cerrado (Bactris setosa Mart.) is a Brazilian fruit that contains several phenolic compounds. This study investigated the effect of tucum aqueous extract in Caco-2 cells in comparison to primary human intestinal organoids and fibroblasts. Cells were exposed to 0.5 and 1 mg/ml of tucum aqueous extract for 24 h. ROS production, mRNA levels for SOD1 and SOD2, CAT, GPX1, NFE2L2, HIF1A and NOS2 were evaluated in Caco-2 cells exposed to tucum extract. Cell viability of Caco-2 cells was decreased upon tucum extract exposure. Mitochondrial ROS levels increased in Caco-2 cells exposed to tucum extract. The mRNA levels of SOD1, SOD2, CAT, GPX, NFE2L2 and HIF1A were downregulated in Caco-2 cells exposed to tucum extract, while NOS2 mRNA levels remained unchanged. Protein levels of SOD2, CAT and NRF2 remained unchanged in Caco-2 cells treated with tucum extract, indicating that catalase and SOD2 cellular functions may be unaffected by the tucum extract at 24 h, of exposure. Aqueous extract of tucum-do-cerrado may induce cellular toxicity in a cancer cell-specific manner, possibly through increased mitochondrial ROS production and gene expression regulation.

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A Combination of Rosa Canina Extracts and Gold Complex Favors Apoptosis of Caco-2 Cells by Increasing Oxidative Stress and Mitochondrial Dysfunction

Cited by 12 publications

References 33 publications

Grape Stem Extracts with Potential Anticancer and Antioxidant Properties

Grape Stem Extracts with Potential Anticancer and Antioxidant Properties

Dually Active Apigenin-Loaded Nanostructured Lipid Carriers for Cancer Treatment

Pro-Oxidant and Cytotoxic Effects of Tucum-Do-Cerrado (Bactris setosa Mart.) Extracts in Colorectal Adenocarcinoma Caco-2 Cells

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