A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes

Poschmann, Gereon; Lendzian, Anna; Uszkoreit, Julian; Eisenacher, Martin; Borght, Ann Vander; Ramaekers, Frans C. S.; Meyer, Helmut E.

doi:10.3109/13813455.2013.789529

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…To cope with winter or cold conditions, small mammals always enhance nonshivering thermogenesis (NST), and the major site of its occurrence is brown adipose tissue (BAT). Long-term cold exposure also leads to "browning" in white adipose tissue (WAT), which produces BAT-like properties (Peschechera & Eckel 2013), and transcriptional activation of many genes involved in lipid catabolism (Christian 2005;Kim et al 2017). In the past, mammalian adipose tissues were mainly divided into WAT and BAT, but in recent years another adipose tissue was found, which is induced from WAT, called "beige adipose tissue" (Wang et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

Zhang

Zhu

et al. 2019

The European Zoological Journal

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Animals employ various strategies to adapt to different natural habitats. In order to investigate the relationship between thermogenesis of adipose tissue and seasonal variations in Tupaia belangeri, body mass, food intake, adipose tissue morphology, and expression levels of three adipose differentiation-related genesperoxisome proliferators-activated receptor α (PPARα), cyclooxygenase-2 (COX-2) and peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α)were measured in the present study. The results show that body mass and food intake increased significantly in winter compared with summer. White adipose tissue (WAT) in winter demonstrated the characteristic appearance of beige adipocytes, including smaller and multi-chamber lipid droplets, darker hue, and increased expression levels of COX-2 and PGC-1α genes, which would increase thermogenic properties in T. belangeri to make up for the lack of thermogenesis in nonshivering thermogenesis (NST) caused by brown adipose tissue (BAT). Moreover, analysis of BAT morphology showed smaller lipid droplets, and COX-2 and PPARα gene expression in BAT increased significantly in winter, indicating that uncoupling protein 1 (UCP1) had been activated to increase NST. All of these results suggest that T. belangeri enhanced thermogenic capacity in winter, including WAT undergoing "browning" and enhanced NST in BAT, which played important roles in the strategies of thermal physiology and bioenergetics in T. belangeri during seasonal change.

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Section: Introductionmentioning

confidence: 99%

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

Zhang

Zhu

et al. 2019

The European Zoological Journal

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“…By contrast, HL-60 cells treated with 1.25 mg/l DADS for 12 h exhibited an increase in DJ-1 expression in cytoplasm and decreased expression in the nucleus when compared with cells treated with 1.25 mg/l DADS for 8 h. These results suggest that DJ-1 protein may be translocated back from the cell nucleus into the cytoplasm following 8 h of DADS exposure. Immunocytochemistry and immunofluorescence assay analysis results confirmed the observed translocation of DJ-1 from the cytoplasm to the nucleus following exposure to DADS for 8 h. Proteins are often distributed in different subcellular locations, which is associated with their different functions (27). A previous study demonstrated that the DJ-1 protein, which is considered to be involved in the regulation of gene transcription, translocates from the cytoplasm to the nucleus during S phase of the cell cycle upon mitogen stimulation (28).…”

Section: Discussionmentioning

confidence: 56%

Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment

Tang²,

Qin

et al. 2016

Molecular Medicine Reports

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Diallyl disulfide (DADS) has been demonstrated to exert potent anticancer effects in vitro and in vivo. Previous studies indicate that DADS may induce the differentiation and/or apoptosis of human leukemia cells in vitro. However, the mechanisms underlying these anticancer effects remain elusive. The aim of the present study was to investigate alterations in the subcellular localization of protein deglycase DJ-1 (also known as Parkinsonism associated deglycase-7, PARK-7) in the cytoplasm, nucleus and mitochondria of human leukemia HL-60 cells induced by DADS, in order to provide novel experimental evidence for the molecular mechanisms underlying the anticancer mechanisms of DADS in leukemia cells. HL-60 cells induced by DADS were collected at different time points, and proteins from the cytoplasm, nucleus and mitochondria of the cells were isolated using specific cellular component isolation kits. The protein expression levels of DJ-1 in these subcellular fractions of HL60 cells following exposure to DADS for varying lengths of time, were determined using western blotting, immunocytochemistry and immunofluorescence techniques. Following exposure of HL-60 cells to 1.25 mg/l DADS for 8 h, the protein expression levels of DJ-1 were significantly decreased in the cytoplasm, while nuclear fractions exhibited a significant increase in DJ-1 expression when compared with untreated controls. The protein expression levels of DJ-1 in mitochondria of HL-60 cells were significantly decreased following treatment with 5 and 10 mg/l DADS. These results demonstrate that exposure of HL-60 cells to low concentrations of DADS may promote DJ-1 protein translocation from the cytoplasm to the nucleus, which suggests that DJ-1 may function as a transcription factor or cofactor binding protein in the process of cell differentiation. The expression of DJ-1 in mitochondria may be associated with induction of apoptosis in HL-60 cells treated with moderate doses of DADS.

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“…For enrichment analysis, p-values were corrected according to Benjamini Hochberg if appropriate and a list of proteins identified from a comparison of small cell lung cancer cell lines [25] was used as background list to exclude a bias introduced by the 2D-gel based method favoring higher abundant and non-membranous proteins.…”

Section: Protein Identification By Mass Spectrometrymentioning

confidence: 99%

Redox proteomics reveal stress responsive proteins linking peroxiredoxin-1 status in glioma to chemosensitivity and oxidative stress

Poschmann

Grzendowski

Stefanski

et al. 2015

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

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A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes

Abstract: Additional CTAB-SDS page is a complementary method to IEF-SDS page revealing a complete new subset of proteins differentially expressed between GLC1 and GLC1 M13 cells SCLC subtypes.

Cited by 3 publications

References 63 publications

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

Seasonal variations of adipose tissue in Tupaia belangeri (Mammalia: Scandentia: Tupaiidae)

Subcellular localization of DJ-1 in human HL-60 leukemia cells in response to diallyl disulfide treatment

Redox proteomics reveal stress responsive proteins linking peroxiredoxin-1 status in glioma to chemosensitivity and oxidative stress

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