A combined additive layer manufacturing / indirect replication method to prototype 3D vascular-like structures of soft tissue and endocrine organs

Bassoli, Elena; Denti, Lucia; Gatto, Andrea; Spaletta, Giulia; Paderno, Alessandro; Zini, Nicoletta; Parrilli, Annapaola; Giardino, Roberto; Strusi, Valentina; Dallatana, D.; Mastrogiacomo, Simone; Zamparelli, Alessandra; Iafisco, Michele; Toni, Roberto

doi:10.1080/17452759.2012.668701

Cited by 14 publications

(5 citation statements)

References 19 publications

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“…As we have previously tested in a number of organ's model, 25,26,[71][72][73][74] we perfused the scapular vascular pedicles with a solution of thermoplastic material, the biocompatible polylevolactic acid (PLLA). 28 Then, based on an hoc developed modification of a phase dark-field contrast light microscopic technique, 30 we observed a striking birefringence of the polymerized PLLA lining blood vessel walls in the decellularized cortical compartment, as expected for the crystalline structure of this polymer.…”

Section: Cell Compatibility Of the 3d Scapular Organoidmentioning

confidence: 99%

A bioartificial and vasculomorphic bone matrix‐based organoid mimicking microanatomy of flat and short bones

Toni,

Barbaro,

Di Conza

et al. 2023

J Biomed Mater Res

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We engineered an in vitro model of bioartificial 3D bone organoid consistent with an anatomical and vascular microenvironment common to mammalian flat and short bones. To achieve this, we chose the decellularized–decalcified matrix of the adult male rat scapula, implemented with the reconstruction of its intrinsic vessels, obtained through an original intravascular perfusion with polylevolactic (PLLA), followed by coating of the PLLA‐fabricated vascularization with rat tail collagen. As a result, the 3D bone and vascular geometry of the native bone cortical and cancellous compartments was reproduced, and the rat tail collagen–PLLA biomaterial could in vitro act as a surrogate of the perivascular extracellular matrix (ECM) around the wall of the biomaterial‐reconstituted cancellous vessels. As a proof‐of‐concept of cell compatibility and site‐dependent osteoinductive properties of this bioartificial 3D construct, we show that it in vitro leads to a time‐dependent microtopographic positioning of rat mesenchymal stromal cells (MSCs), initiating an osteogenic fate in relation to the bone compartment. In addition, coating of PLLA‐reconstructed vessels with rat tail collagen favored perivascular attachment and survival of MSC‐like cells (mouse embryonic fibroblasts), confirming its potentiality as a perivascular stroma for triggering competence of seeded MSCs. Finally, in vivo radiographic topography of bone lesions in the human jaw and foot tarsus of subjects with primary osteoporosis revealed selective bone cortical versus cancellous involvement, suggesting usefulness of a human 3D bone organoid engineered with the same principles of our rat organoid, to in vitro investigate compartment‐dependent activities of human MSC in flat and short bones under experimental osteoporotic challenge. We conclude that our 3D bioartificial construct offers a reliable replica of flat and short bones microanatomy, and promises to help in building a compartment‐dependent mechanistic perspective of bone remodeling, including the microtopographic dysregulation of osteoporosis.

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Section: Cell Compatibility Of the 3d Scapular Organoidmentioning

confidence: 99%

A bioartificial and vasculomorphic bone matrix‐based organoid mimicking microanatomy of flat and short bones

Toni,

Barbaro,

Di Conza

et al. 2023

J Biomed Mater Res

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“…After the master vascular tree had been coated with a metallic shield and emptied by thermal dissolution, the resulting vascular spaces were prepared for eventual refi lling with PCL and PLLA under vacuum conditions, in order to obtain an accurate biocompatible replica of the 3D vessel geometry. Finally, the copper shield was deoxidized with ammonia, and electrolyzed by current fl ow reversal at specifi c frequency (Bassoli et al , 2012b). Similar procedures are being investigated for the replication of the intraglandular arterial network of the adult human thyroid with biomaterials.…”

Section: Studies On the Fabrication Of Vascular-like Reticular Scaffoldsmentioning

confidence: 99%

Biomimetic materials in regenerative medicine

Sprio

Sandri

Iafisco

et al. 2013

Biomimetic Biomaterials

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“…The results showed that this method had advantages over other rebuilding methods in terms of accuracy, speed, safety and cost. E. Bassoli et al, 17 reported a combined 3D printing/indirect replication method to fabricate 3D vascular-like structures of soft tissue 17 ; using the ProJet® HD 3000 3D printer from 3D Systems Inc. to print the negative mold of the vascular structure.…”

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confidence: 99%

Fabrication of low cost soft tissue prostheses with the desktop 3D printer

Xue

2014

Sci Rep

208

140

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Soft tissue prostheses such as artificial ear, eye and nose are widely used in the maxillofacial rehabilitation. In this report we demonstrate how to fabricate soft prostheses mold with a low cost desktop 3D printer. The fabrication method used is referred to as Scanning Printing Polishing Casting (SPPC). Firstly the anatomy is scanned with a 3D scanner, then a tissue casting mold is designed on computer and printed with a desktop 3D printer. Subsequently, a chemical polishing method is used to polish the casting mold by removing the staircase effect and acquiring a smooth surface. Finally, the last step is to cast medical grade silicone into the mold. After the silicone is cured, the fine soft prostheses can be removed from the mold. Utilizing the SPPC method, soft prostheses with smooth surface and complicated structure can be fabricated at a low cost. Accordingly, the total cost of fabricating ear prosthesis is about $30, which is much lower than the current soft prostheses fabrication methods.

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A combined additive layer manufacturing / indirect replication method to prototype 3D vascular-like structures of soft tissue and endocrine organs

Abstract: 2012) A combined additive layer manufacturing / indirect replication method to prototype 3D vascular-like structures of soft tissue and endocrine organs, Virtual and Physical Prototyping, 7:1, 3-11,

Cited by 14 publications

References 19 publications

A bioartificial and vasculomorphic bone matrix‐based organoid mimicking microanatomy of flat and short bones

A bioartificial and vasculomorphic bone matrix‐based organoid mimicking microanatomy of flat and short bones

Biomimetic materials in regenerative medicine

Fabrication of low cost soft tissue prostheses with the desktop 3D printer

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