A combined AgNOR-Feulgen staining technique.

Foucrier, Jean; Rigaut, Jean Paul; Pechinot, Danielle

doi:10.1177/38.11.1698851

Cited by 6 publications

(2 citation statements)

References 19 publications

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“…NOR-silver staining has been described as a cytochemical method for light and electron microscopy (Foucrier et al, 1990;Rowlands et al, 1990;Hubbell, 1985;Moreno et al. 1985;Thiebaut et al.…”

Section: Discussionmentioning

confidence: 99%

Procedures for specific detection of silver-stained nucleolar proteins on western blots.

Hozák

Roussel²,

Hernandez‐Verdun³

1992

J Histochem Cytochem.

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Nucleolar organizer region (NOR)-dm staiaing of the chromosomes and nucleoli is a method that enables the detection of proteins associated with the ribosomal genes. We adapted the most commonly used qtochemical NOR-silver staining techniques to Western-blotted proteins ofHeLa cells, mimicking the silver staining of c e h in situ, and testing several parameters that may influence the in situ reaction. Two of these techniques, both one-step methods with colloidal developers, were standardized to obtain reproducible results. The specificity of NOR staining is documented by: (a) only a few bands are r d e d among the many proteins detected by total proteins staining on geh or blots; two major groups of bands are found around 100 KD and 40 w that could correspond at least in part to nucleolin and B23 nucleolar proteins; (b) the silver staining of bands was not the result of the high relative protein concentrations; and (c) the same

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Section: Discussionmentioning

confidence: 99%

Procedures for specific detection of silver-stained nucleolar proteins on western blots.

Hozák

Roussel²,

Hernandez‐Verdun³

1992

J Histochem Cytochem.

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“…The technique was simplified by Howell and Black (1980) and has been applied to sections for light (Ploton et al, 1986;Moreno et al, 1988) and electron microscopy (Moreno et al, 1985). It has also been used to stain cytological smears (Foucrier et al, 1990), monolayer cell cultures , chromatin spread preparations (Angelier et al, 1982;Roussel et al, 1992) and electrophoretically separated proteins either on gels (Lischwe et al, 1979) or on Western blots (Hozak et aI., 1992;Martin et aI., 1992).…”

Section: Introductionmentioning

confidence: 98%

A new version of the Ag−NOR technique. A combination with DAPI staining

Biliński

Bilińska

1996

Histochem J

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The Ag-NOR staining technique is widely used for visualizing nucleolar organizer regions (NORs) in various plant and animal tissues. We describe a simple and time-saving combination of Ag-NOR staining with DNA detection by fluorescence microscopy. This modification was tested on cultured cells and semi-thin sections of plastic-embedded tissues. Of the different fixatives and embedding media used in our studies, the best results (i.e., high selectivity of staining, and lack of or very low background precipitation) were obtained with fixation in methanol-acetone at-20 degrees C for cultured cells, and fixation in 4% formaldehyde followed by embedding in Histocryl resin for tissue sections. The optimal time of Ag-NOR staining was determined experimentally for all materials tested. The specificity of the staining was checked at the electron microscopical level. Especially good results were obtained by mixing epifluorescence with standard bright-field illumination. In such a combination, Ag-NOR-positive nucleoli, or their fibrillar centres and dense fibrillar components, were clearly visible against a bright background of nuclear DNA.

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Correlationship between cellular DNA and AgNOR protein content in the developing course of colorectal adenocarcinoma

Xie

Hanting

1995

Chinese Journal of Cancer Research

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A combined AgNOR-Feulgen staining technique.

Cited by 6 publications

References 19 publications

Procedures for specific detection of silver-stained nucleolar proteins on western blots.

Procedures for specific detection of silver-stained nucleolar proteins on western blots.

A new version of the Ag−NOR technique. A combination with DAPI staining

Correlationship between cellular DNA and AgNOR protein content in the developing course of colorectal adenocarcinoma

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