A combined analysis of XRCC1, XRCC3, GSTM1 and GSTT1 polymorphisms and centromere content of micronuclei in welders

Iarmarcovai, G.; Sari-Minodier, I.; Orsière, Thierry; Méo, Michel De; Gallice, Philippe; Bideau, Chantal; Iniesta, Danielle; Pompili, J.; Bergé-Lefranc, Jean-Louis; Botta, A.

doi:10.1093/mutage/gel010

Cited by 33 publications

(24 citation statements)

References 54 publications

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“…The available data have shown a trend towards no differences between these 2 mechanisms of MN formation [27]. Despite the relatively small sample size, especially after stratification into subgroups, our data demonstrate that subjects with a history of UCC harbor and accumulate genetically unstable cells in the bladder urothelium that may represent early precursors of new UCC or subclones from previous UCC.…”

Section: Discussionmentioning

confidence: 49%

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

et al. 2014

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Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.

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Section: Discussionmentioning

confidence: 49%

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

et al. 2014

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“…In the literature, there are few in vitro studies regarding peripheral lymphocytes of welders (12)(13)(14), and this study will contribute to the literature. Because the genotoxicity was reported with the parameters of the sister chromatid exchange, the chromosome aberration, and the polymorphisms in these studies, this study is the first report for genotoxicity with the parameters of MN and RI in welders.…”

Section: Discussionmentioning

confidence: 93%

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal-arc welding

Şener¹,

Eroğlu

2013

Turk J Med Sci

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To foster the practical development of the constructed wetlands used for water quality enhancement in Turkey, 2 vertical subsurface flow pilot-scale constructed wetlands were implemented on the METU campus, Ankara, Turkey. Both of the wetlands were planted with Phragmites australis and operated identically at a flowrate of 3 m 3 .d −1 and a hydraulic loading rate (HLR) of 0.100 m.d −1 , intermittently. The main objective of the research was to quantify the effect of different substrates (gravel and blast furnace granulated slag) on the nutrient removal performance of the constructed wetlands in the prevailing climate of Ankara. According to the monitoring study (July 2002-January 2003), concentration based average removal efficiencies for the slag and gravel reed beds were as follows: TSS (64% and 62%), COD (49% and 40%), NH + 4 -N (88% and 58%), TN (41% and 44%), TP (63% and 9%) and PO 3− 4 -P (60% and 4%). In general, the treatment performance of the slag system was better than that of the gravel system.

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“…For styrene, welding fumes and inorganic arsenite compounds, additional genetic polymorphisms (e.g. xenobiotic metabolism enzymes) may influence MN frequencies (51)(52)(53). The pooled approach, by combining data from several studies, increased the statistical power of the analysis (54), providing a greater potential to understand the contribution of environmental and genetic factors to genome stability.…”

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confidence: 99%

hOGG1326, XRCC1399 and XRCC3241 polymorphisms influence micronucleus frequencies in human lymphocytes in vivo

et al. 2007

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A pooled analysis of five biomonitoring studies was performed to assess the influence of hOGG1 326 , XRCC1 399 and XRCC3 241 gene polymorphisms on micronuclei (MN) frequency in human peripheral blood lymphocytes, as measured by the ex vivo/in vitro cytokinesis-block micronucleus (CBMN) assay. Each study addressed a type of occupational exposure potentially able to induce DNA strand breakage (styrene, ionising radiation, cobalt/hard metal, welding fumes and inorganic arsenite compounds), and therefore MN, as a result of base excision repair and double-strand break repair deficiencies. The effect of genotype, age, exposure to genotoxic agents and smoking habit on MN induction was determined using Poisson regression analysis in 171 occupationally exposed male workers and in 132 non-exposed male referents. The analysis of genotype-genotype, genotype-smoking and genotype-exposure interactions by linear combinations of parameters showed significantly higher MN frequencies in the following subsets: (i) occupationally exposed workers carrying either the Thr/Thr or the Thr/Met XRCC3 241 genotypes compared to their referent counterparts (P < 0.001) and (ii) carriers of the Met/Met XRCC3 241 genotype compared to Thr/Thr XRCC3 241 carriers, as far as they are non-exposed and bear the variant (Ser/Cys or Cys/Cys) hOGG1 326 genotype (P < 0.01). Significantly lower MN frequencies were observed in carriers of the variant hOGG1 326 genotype compared to Ser/Ser hOGG1 326 carriers in the subgroup of non-smokers with Thr/Thr XRCC3 241 genotype (P < 0.01). Stratified analysis by occupational exposure showed a significant MN increase with smoking in occupationally exposed carriers of the Arg/Gln XRCC1 399 genotype (P < 0.001). In contrast, a significant MN decrease with smoking was observed in referents carrying the Ser/Ser hOGG1 326 genotype (P < 0.01). These findings provide evidence that the combination of different DNA repair genes and their interaction with environmental genotoxic agents may modulate MN induction. Understanding the complexity of the relationships between exposure, DNA repair and MN frequencies require larger scale studies and complementary biomarkers.

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A combined analysis of XRCC1, XRCC3, GSTM1 and GSTT1 polymorphisms and centromere content of micronuclei in welders

Cited by 33 publications

References 54 publications

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

Genetic Instability Persists in Non-Neoplastic Urothelial Cells from Patients with a History of Urothelial Cell Carcinoma

The in vitro determination of genotoxicity in peripheral lymphocytes of welders exposed to fumes from metal-arc welding

hOGG1326, XRCC1399 and XRCC3241 polymorphisms influence micronucleus frequencies in human lymphocytes in vivo

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