A combined pathway of organogenesis and somatic embryogenesis for an efficient large-scale propagation in date palm (Phoenix dactylifera L.) cv. Mejhoul

Mazri, Mouaad Amine; Meziani, Reda; Belkoura, I.; Mokhless, Boutaïna; Nour, Souad

doi:10.1007/s13205-018-1235-x

Cited by 34 publications

(23 citation statements)

References 32 publications

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“…Such explants, which are pathogen-free and available throughout the year, were successfully used previously in other Moroccan date palm cultivars. 8,9,11 The findings of the present investigation indicated that adventitious bud explants have a high embryogenic potential, with a rate of up to 89%. This is in good agreement with our previous studies.…”

Section: Discussionmentioning

confidence: 50%

“…10 Recently, explants derived from plant material maintained in vitro, such as adventitious buds, leaves, roots were also used, and a high embryogenic potential of adventitious bud explants was reported. 8,11 Such explants are pathogen-free, available independently of seasons and allow to avoid excessive use of offshoots and spathes. 8 The main objective of the present work was to develop a regeneration system for date palm cv.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

et al. 2019

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An efficient somatic embryogenesis system is reported for date palm cv. Al-Fayda, a genotype resistant to the bayoud disease. Callus induction was achieved from adventitious bud explants cultured for 6 months on semi-solid Murashige and Skoog (MS) medium containing 4.5 μM 6-(dimethylallylamino) purine (2iP) and various concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) or picloram. The highest somatic embryogenesis frequency (89%) was obtained on MS medium supplemented with 225 μM 2,4-D. Subsequently, embryogenic cultures were transferred to agitated liquid MS medium (maturation medium) containing various concentrations of mannitol, polyethylene glycol (PEG) or sorbitol. The highest rate of somatic embryo maturation (71.4 mature embryos per 100 mg callus) was achieved on the medium supplemented with 40 g l-1 PEG. Mature somatic embryos were then transferred to MS medium supplemented with gibberellic acid (GA3) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) at various concentrations. The highest frequency of germination and conversion (26%) was obtained on the medium containing 5 μM NAA and 5 μM BAP. The developed plants were then transferred to ex vitro conditions, where a survival rate of 77.02% was observed. The regeneration protocol established in the present investigation will be used for mass propagation of date palm cv. Al-Fayda.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

et al. 2019

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“…Results clearly showed that combinations of picloram with 2ip and BA gave the highest response for embryonic culture and globularization. Similarly, inducing somatic embryogenesis from adventitious bud segments of Mejhoul shoot tips was encouraged by different concentrations of picloram [ 36 ]. The surpassed medium for somatic embryogenesis induction (70%) was MS medium supplemented with 10.8 mg/l picloram and 1.0 mg/l 2iP.…”

Section: Discussionmentioning

confidence: 99%

High-frequency direct somatic embryogenesis and plantlet regeneration from date palm immature inflorescences using picloram

Hassan

Allam

Din³

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Date palm (Phoenix dactylifera L.) is a traditional crop in arid and semi-arid areas. Its vegetative propagation can be achieved by offshoots, but possible number of offshoots in mother palm trees is limited. Micropropagation is a highly recommended strategy for obtaining date palm elite cultivars using shoot tip and immature inflorescences. In this study, micropropagation procedure using inflorescence explants of Medjool cv. is described. For culture initiation, explants from different spathe lengths were cultivated on Murashige and Skoog medium (MS) supplemented with picloram at 1.0 and 2.0 mg/l combined with 2iP at 0.5 mg/l alone and with both 2iP and BA at 0.25 mg/l for 24 weeks. The obtained direct globular embryos were transferred to maturation media with 0.1 mg/l picloram alone or combined with both 2iP and ABA separately and together for further development. Additionally, multiplication and rooting media were optimized by different cytokinins and auxins for high frequency of plantlet production. Acclimatization of in vitro plantlets was also investigated. Results The highest percentage of globular embryo formation was noticed with explants isolated from spathe lengths ranging from 10 to 15 cm. Addition of BA to initiation media with picloram encouraged a significant effect on embryonic culture formation percentage. Incorporation of ABA and 2iP to maturation medium was an effective factor for individual or multiple embryo emergence. Acclimatization of in vitro plantlets having 3–4 roots was successfully accomplished. Irrigation with the full strength solution (MS) encouraged the highest growth vigor degree, leaf number/plant, leaf width, root number, and root thickness degree of ex vitro plants. Conclusion This research provides an advanced regeneration system for large-scale production of date palm from immature inflorescences of Medjool cv. It opens up the prospects of using picloram with different growth regulators for rapid micropropagation of date palm.

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“…When a PGR stimulates explants and calluses, the internal intrinsic hormones produce feedback [12] and induce a callus to form an embryonic callus [13,14]. In many cases, specific combinations and proportions of auxin and cytokinin can effectively induce embryonic callus or directional differentiation of organs [15,16]. Somatic embryogenesis technology plays an important role in the preparation of artificial seeds and the genetic improvement of garden plants.…”

Section: Introductionmentioning

confidence: 99%

The regeneration of Acer rubrum L. “October Glory” through embryonic callus

et al. 2020

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Background: Tissue culture and rapid propagation technology is an important way to solve the difficulties of plant propagation. This experiment aims to explore the appropriate conditions at each stage of the red maple's tissue culture process and to obtain plantlets, thus providing a theoretical basis for the establishment of the red maple's tissue culture system. Results: The results showed that the stem segment is the most suitable explant for inducing embryogenic callus. The MS (Murashige&Skoog) + 0.8 mg/L TDZ (Thidiazuron) + 1.0 mg/L 6-BA (6-Benzylaminopurine) + 0.5 mg/L IAA(Indole-3-acetic acid) + 35 g/L sucrose+ 7.5 g/L semi-fixed medium was the best for callus formation. When selecting type VI callus as embryonic callus induction material, MS + 0.6 mg/L TDZ + 0.5 mg/L 6-BA + 2.0 mg/L IAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS + 1.0 mg/L TDZ + 3.0 mg/L 6-BA+ 0.2 mg/L NAA (1-Naphthaleneacetic acid) + 1.2 mg/L IAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium. The induction rate of adventitious roots in MS + 0.6 mg/L TDZ + 1.0 mg/L 6-BA+ 3 mg/L NAA + 35 g/L sucrose+ 7.5 g/L semi-fixed medium was the highest, reaching 76%. Conclusions: In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be sub-cultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.

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A combined pathway of organogenesis and somatic embryogenesis for an efficient large-scale propagation in date palm (Phoenix dactylifera L.) cv. Mejhoul

Cited by 34 publications

References 32 publications

Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

Impact of Osmotica and Plant Growth Regulators on Somatic Embryogenesis of Date Palm

High-frequency direct somatic embryogenesis and plantlet regeneration from date palm immature inflorescences using picloram

The regeneration of Acer rubrum L. “October Glory” through embryonic callus

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