A combined quality evaluation method that integrates chemical constituents, appearance traits and origins of raw Rehmanniae Radix pieces

Gu, Min; Yang, Yuan; Qin, Zinan; Xu, Youzhi; Shi, Nannan; Wang, Yanping; Zhai, Huaqiang; Qian, Zhong-Zhi

doi:10.1016/s1875-5364(21)60056-0

Cited by 6 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As for compounds which had low content, especially Ginseng Radix et Rhizoma, Rehmanniae Radix, and Poria, or were difficult to be completely separated, we used LC-MS/MS to quantify them. The candidate ingredients were chosen based on the references of pertinent herbs, including the following 14 chemical components: XS and XZ from the minister medicine of Xanthii fructus [ 21 ]; JA and JB from the minister medicine of Ziziphi Spinosae Semen [ 22 ]; MPA from the minister medicine of Ophiopogonis Radix [ 23 ]; RD from the minister medicine of Rehmanniae Radix [ 24 ]; GRF, GRG, GRR, and GRB from the minister medicine of Ginseng Radix et Rhizoma [ 25 ]; DA, PAC, and ATA from the adjuvants medicine of Poria [ 26 ]; and PX from adjuvants medicine of the guide medicine of Polygalae Radix [ 27 ]. The chemical structures of the 25 compounds are shown in Figure S5 .…”

Section: Resultsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.

show abstract

Section: Resultsmentioning

confidence: 99%

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Yang²,

Cui³

et al. 2022

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

show abstract

“…It has the effects of clearing heat and cooling blood, replenishing the essence and marrow, lowering blood sugar, enhancing immunity, and improving hematopoietic activity [3,4]. It is widely used to treat diseases, such as hypertension, autoimmune meningitis, osteoporosis, blood deficiency, constipation, and diabetes [5][6][7][8]. The main components of Rehmannia glutinosa are oligosaccharides, polysaccharides, and iridoid glycosides.…”

Section: Introductionmentioning

confidence: 99%

Difference analysis of oligosaccharides in different varieties of Rehmannia glutinosa

Zhao,

Song,

Cai

et al. 2024

Food Nutr. Chem.

View full text Add to dashboard Cite

The oligosaccharide and monosaccharide contents of different varieties of Rehmannia glutinosa were compared to explore the difference in the oligosaccharide and monosaccharide contents in different varieties and to provide a scientific basis for formulation of product quality standards of Rehmannia glutinosa. The contents of fructose, glucose, sucrose, raffinose, and stachyose were determined via high-performance liquid chromatography. The total sugar content in the fresh Rehmannia glutinosa varieties in descending order was as follows: Beijing Tuodu 3 > Huaifeng > Jinjiu > Huaizhong 1 > Beijing 3. The total sugar content in the raw Rehmannia glutinosa varieties in descending order was as follows: Jinjiu > Huaifeng > Beijing Tuodu 3 > Beijing 3 > Huaizhong 1. The stachyose content was the highest among the five oligosaccharides. The total sugar content in the Beijing Tuodu 3 variety was the highest among the fresh Rehmannia glutinosa varieties, and the total sugar content in the Jinjiu variety was the highest among the raw Rehmannia glutinosa varieties. There were significant differences in the oligosaccharide and monosaccharide contents among the different varieties.

show abstract

“…Currently, the common method for quality assessment is to detect the content of a representative component. 6 But there are two prominent problems in the practical application of this method. One is that the selected component is inherently unstable and may not even be detected due to the influence of temperature, process, etc.…”

Section: ■ Introductionmentioning

confidence: 99%

“…So it is a good way to evaluate and distinguish R. glutinosa by chemical composition. Currently, the common method for quality assessment is to detect the content of a representative component . But there are two prominent problems in the practical application of this method.…”

Section: Introductionmentioning

confidence: 99%

Combination of Internal Extractive Electrospray Ionization Mass Spectrometry and Statistical Analysis for High-Throughput Molecular Differentiation of Rehmannia glutinosa Samples

Xu,

Yu,

et al. 2023

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey. is a perennial herb of the Scrophulariaceae family, which has long enjoyed a good reputation in China, and has a wide range of pharmacological effects and clinical applications. The place of origin is an important factor affecting the chemical composition of R. glutinosa, resulting in different pharmacological effects. Herein, internal extractive electrospray ionization mass spectrometry (iEESI-MS) combined with statistical techniques was established for high-throughput molecular differentiation of different R. glutinosa samples. Dried and processed R. glutinosa samples from four different places of origin were analyzed by iEESI-MS with high throughput (>200 peaks) and rapidness (<2 min/sample) without sample pretreatment. Clear separation models created by OPLS-DA were then established for distinguishing the places of origin of dried and processed R. glutinosa by using the obtained MS data. In addition, the molecular differences between the pharmacological effects of dried and processed R. glutinosa were also investigated by OPLS-DA, and 31 different components were screened out. This work provides a promising method for evaluating the quality of traditional Chinese medicines and studying the biochemical mechanism of processing.

show abstract

A combined quality evaluation method that integrates chemical constituents, appearance traits and origins of raw Rehmanniae Radix pieces

Cited by 6 publications

References 21 publications

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics

Difference analysis of oligosaccharides in different varieties of Rehmannia glutinosa

Combination of Internal Extractive Electrospray Ionization Mass Spectrometry and Statistical Analysis for High-Throughput Molecular Differentiation of Rehmannia glutinosa Samples

Contact Info

Product

Resources

About