A combined transcriptomic and proteomic analysis of chrysanthemum provides new insights into petal senescence

Yao, Juanni; Li, Rui; Cheng, Yulin; Li, Zhengguo

doi:10.1007/s00425-021-03808-9

Cited by 11 publications

(5 citation statements)

References 61 publications

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“…A total of 11,324 DEGs and 403 DEPs were identified by omics analysis in the senescence degree of chrysanthemum petals. The author speculates an involvement of transcriptional and epigenetic regulation in petal senescence [ 70 ]. RNA-seq and iTRAQ analysis of aphids before and after CMV (cucumber mosaic virus) infection identified 20,550 DEGs, however, only 744 DEPs were obtained [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line

Wang

Cheng

Zhai

et al. 2023

IJMS

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Male sterility is a common phenomenon in the plant kingdom and based on the organelles harboring the male-sterility genes, it can be classified into the genic male sterility (GMS) and the cytoplasmic male sterility (CMS). In every generation, CMS can generate 100% male-sterile population, which is very important for the breeders to take advantage of the heterosis and for the seed producers to guarantee the seed purity. Celery is a cross-pollinated plant with the compound umbel type of inflorescence which carries hundreds of small flowers. These characteristics make CMS the only option to produce the commercial hybrid celery seeds. In this study, transcriptomic and proteomic analyses were performed to identify genes and proteins that are associated with celery CMS. A total of 1255 differentially expressed genes (DEGs) and 89 differentially expressed proteins (DEPs) were identified between the CMS and its maintainer line, then 25 genes were found to differentially expressed at both the transcript and protein levels. Ten DEGs involved in the fleece layer and outer pollen wall development were identified by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, most of which were down-regulated in the sterile line W99A. These DEGs and DEPs were mainly enriched in the pathways of “phenylpropanoid/sporopollenin synthesis/metabolism”, “energy metabolism”, “redox enzyme activity” and “redox processes”. Results obtained in this study laid a foundation for the future investigation of mechanisms of pollen development as well as the reasons for the CMS in celery.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line

Wang

Cheng

Zhai

et al. 2023

IJMS

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“…To acquire more accurate results in our proteomic analysis, proteome, phosphoproteome, and ubiquitylome analyses were performed with the same samples, and phosphorylation and ubiquitination quantifications in phosphoproteome and ubiquitylome were normalized by protein quantifications to eliminate the interference caused by protein abundance differences in the proteome, as previously reported [ 56 ]. Noticeably, our rose proteome identified a total of 6165 proteins, which is more than that in the proteomes of other ornamental plants, including petunias [ 43 ], daylily [ 57 ], and chrysanthemum [ 58 ]. In addition, >10 000 phosphorylation or ubiquitination sites were identified in both the phosphoproteome and ubiquitylome of rose, and the number of phosphorylation or ubiquitination sites was significantly more than in other published PTM studies in plants [ 43 , 59–62 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Li,

Yao,

Ming

et al. 2023

Horticulture Research

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As two of the most abundant post-translational modifications (PTMs), phosphorylation and ubiquitination play a significant role in modulating plant–pathogen interactions and increasing evidence indicates their crosstalk in plant immunity. Rose (Rosa sp.) is one of the most important ornamental plants and is seriously infected by Botrytis cinerea. Here, integrated proteomics analysis was performed to detect global proteome, phosphorylation, and ubiquitination changes in rose upon B. cinerea infection and investigate the possible phosphorylation and ubiquitination crosstalk. A total of 6165 proteins, 11774 phosphorylation and 10582 ubiquitination sites, and 77 phosphorylation and 13 ubiquitination motifs were identified. B. cinerea infection resulted in 169 up-regulated and 122 down-regulated proteins, 291 up-regulated and 404 down-regulated phosphorylation sites, and 250 up-regulated and 634 down-regulated ubiquitination sites. There were 12 up-regulated PR10 proteins and half of them also showed reduced ubiquitination. A lot of kinases probably involved in plant pattern-triggered immunity (PTI) signaling were up-regulated phosphoproteins. Noticeably, numerous kinases and ubiquitination-related proteins also showed a significant change in ubiquitination and phosphorylation, respectively. A cross-comparison of phosphoproteome and ubiquitylome indicated that both two PTMs of 104 proteins were dynamically regulated, and many putative PTI signaling components in plant plasma membrane were co-regulated. Moreover, five selected proteins including four PR10 proteins and a plasma membrane aquaporin were proven to be involved in rose resistance to B. cinerea. Our study provides insights into the molecular mechanisms underlying rose resistance to B. cinerea and also increases the database of phosphorylation and ubiquitination sites in plants.

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“…On the rose side, the variety To Dios, with greater resistance to high temperatures, was developed (Heo et al, 2016). Genetic tools also made it possible to describe significant morphological and genetic differences between buttercup genotypes (Baran et al, 2023), as well as the identification of key genes and proteins related to petal senescence in chrysanthemums (Yao et al, 2021).…”

Section: Genome Study and Genetic Improvementmentioning

confidence: 99%

Scientific analysis of cut flowers: a review of the main technical issues developed

Villagran,

Rocha,

Mojica

et al. 2024

Ornam. Hortic.

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The cut flower sector is an area of horticulture that generates great economic and academic interest worldwide, the production of flowers represents a market segment that has aesthetic and sentimental purposes in many regions of the world. In recent years, a large amount of research has been generated that has promoted this sector, therefore, in this work we have proposed to develop a systematic review in this field of knowledge, with the objective of consolidating knowledge, guiding future research, and supporting informed decisions, thus contributing to the efficient use of resources and the continuous advancement of the cut flower industry. The systematic review process was divided into six stages, starting with the definition of the search equation and the collection of information from the prestigious Scopus database. Subsequently, a detailed quality and technical analysis of the 532 documents collected was conducted. These documents were grouped into 13 topics that are related to the production system, ranging from propagation techniques, genome study, genetic improvement to topics of current interest such as sustainability. Each of these topics was examined and the results of this analysis led to the preparation of this technical document, which includes relevant aspects that have contributed to the improvement of productivity and productive diversity in species and under different climatic conditions.

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A combined transcriptomic and proteomic analysis of chrysanthemum provides new insights into petal senescence

Cited by 11 publications

References 61 publications

Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line

Transcriptomic and Proteomic Analyses of Celery Cytoplasmic Male Sterile Line and Its Maintainer Line

Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

Scientific analysis of cut flowers: a review of the main technical issues developed

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